Detergent compositions containing bacillus sp. mannanase and methods of use thereof

A mannanase and detergent technology, applied to detergent compositions containing Bacillus mannanase and its application field, can solve the problem that the mannanase has limited pH and/or temperature ranges, and is not suitable for formulation and washing conditions, etc.

Inactive Publication Date: 2014-01-08
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, mannanases may have a limited pH and / or temperature range in which they are active, which may make them unsuitable for certain formulations and wash conditions

Method used

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  • Detergent compositions containing bacillus sp. mannanase and methods of use thereof
  • Detergent compositions containing bacillus sp. mannanase and methods of use thereof
  • Detergent compositions containing bacillus sp. mannanase and methods of use thereof

Examples

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example

[0256] The following examples are provided to demonstrate and illustrate certain preferred embodiments and aspects of the invention and should not be construed as limiting.

[0257] In the following experimental disclosures, the following abbreviations are used: M (mole / liter), mM (millimol / liter), μM (micromoles / liter), nM (nanomoles / liter), mol (mole), mmol (mmol ), μmol (micromole), nmol (nanomole), g and gm (gram), mg (milligram), μg (microgram), pg (picogram), L (liter), ml and mL (milliliter), μl and μL (microliter), cm (centimeter), mm (millimeter), μm (micrometer), nm (nanometer), U (unit), MW (molecular weight), s (second), min (minute), h (hour ), ℃ (Celsius), QS (sufficient), ND (not performed), rpm (rev / min), H 2 O (water), dH 2 O (deionized water), HCl (hydrochloric acid), aa (amino acid), bp (base pair), kb (kilobase pair), kD (kilodalton), MgCl 2 (magnesium chloride), NaCl (sodium chloride), Ca (calcium), Mg (magnesium), HEPES (4-(2-hydroxyethyl)-1-piperazine...

example 1

[0259] Cloning of Glycosyl Hydrolase Bsp Man4 from Bacillus sp. SWT81

[0260] Bacillus SWT81 was selected as a potential source of various glycosyl hydrolases and other enzymes that can be used in industrial applications. First, culture Bacillus SWT81 on GAM agar plate (Jones et al., IJSEM (International Journal of Systematic and Evolutionary Microbiology), 55:1711-1714, 2005) at 37°C for 24 hours to obtain the genome for sequencing DNA. Cellular material was scraped off the plate and used to prepare genomic DNA using the ZF Fungal / Bacterial DNA Mini Kit from Zymo (Cat# D6005). Genomic DNA was used for genome sequencing and amplification of the Bsp Man4 gene for expression cloning. The whole genome of Bacillus sp. SWT81 strain was used Sequencing by synthesis (SBS) technology (www.baseclear.com / sequencing / illumina-sequencing / ) was used for sequencing. Genome sequencing and assembly of sequence data were performed by BaseClear (Leiden, The Netherlands). Contigs were an...

example 2

[0329] Expression of Bacillus Glycosyl Hydrolase (Bsp Man4)

[0330] The Bsp Man4 gene was amplified from genomic DNA of Bacillus using the following primers: Primer 1 (BssHII) 5'-TGAGCGCGCA GGCAGCTGGT AAATCACAAG AAGGGCGTCA ACT-3' (SEQ ID NO: 3) and Primer 2 (XhoI) 5'-CGCCTCGAGT TACACTGTTT TTCTTCTTTT AT-3' (SEQ ID NO: 4). After digestion with BssHII / XhoI, the PCR product was cloned into the p2JM103BBI expression vector (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) digested with the same restriction enzymes. Ligation of this DNA fragment to the PCR amplified gene encoding the mature Bsp Man4 protein resulted in the addition of three codons at the 3' end of the nucleic acid encoding the B. subtilis AprE propeptide to the 5' end of the coding region of the mature form of Bsp Man4. The resulting plasmid was labeled pZQ186(aprE-BspMan4). The plasmid map of pZQ186 is as follows figure 1 shown. The recombinant Bsp Man4 protein produced in this way has three additional amino ...

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Abstract

The present compositions and methods relate to an endo-B-mannanase cloned from a Bacillus sp., polynucleotides encoding the endo-B-mannanase, and methods of use thereof. Formulations containing the endo-ss-mannanase are highly suitable for use as detergents.

Description

[0001] priority [0002] This patent application claims priority to International Application No. PCT / CN2011 / 073559 filed on April 29, 2011, which is hereby incorporated by reference in its entirety. technical field [0003] Compositions and methods of the invention relate to endo-beta-mannanases cloned from Bacillus species, polynucleotides encoding endo-beta-mannanases, and methods of use thereof. Formulations comprising endo-beta-mannanase are very suitable for use as detergents. Background technique [0004] Current laundry detergent and fabric care compositions include ingredients such as surfactants, enzymes (proteases, amylases, mannanases and / or cellulases), bleaches, builder systems, suds suppressors Complex combinations of active ingredients such as , suspending agents, soil release agents, optical brighteners, softeners, dispersants, dye transfer inhibiting compounds, abrasives, bactericides and fragrances. [0005] Mannanases, including endo-beta-mannanases, ha...

Claims

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Application Information

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IPC IPC(8): C12N9/42
CPCC12N9/2494A23K20/189C11D3/38636C12C5/004C12Y302/01078
Inventor B·E·琼斯M·科尔克曼钱蓁B·S·劳尔森K·M·克拉格S·普莱斯鲁斯Z·于L·M·巴别M·埃斯塔布鲁克华玲
Owner DANISCO US INC
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