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Culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile

A technology of tissue culture and spring dendrobium, applied in the field of plant cultivation, can solve the problems of great differences among species and difficulty in induction

Inactive Publication Date: 2014-02-05
TIANMU COLLEGE OF ZHEJIANG A&F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are great differences among the varieties of Dendrobium dendrobii, and the induction of adventitious buds is difficult, which is still in the exploratory stage.

Method used

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  • Culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Put 73.2kg of water into the 100L reactor, then put in 1kg calcium chloride, 1kg potassium iodide, 1kg nitric acid, 1kg D-pantothenic acid, 1kg copper sulfate, 1kg ammonium nitrate, 1kg crystalline magnesium sulfate, 0.5kg ferrous sulfate, 0.5kg sulfuric acid Manganese, 0.3kg of zinc sulfate, 0.3kg of molybdenum trioxide and 0.4kg of titanium dioxide were stirred and dissolved, and then slowly added into 1kg of niacin, 1kg of inositol, 1kg of pyridoxine hydrochloride, 1kg of arginine and 1kg of peptone, and stirred evenly. Then add 0.1kg 3-indole acetic acid, 0.1kg 6-furfuryl aminopurine, 0.1kg forchlorfenuron, 0.1kg transzeatin, 0.1kg cyberon, 0.1kg α-naphthalene acetic acid, 0.1kg 2,4-dichlorobenzene Oxyacetic acid, 0.1kg gibberellin, 0.1kg epibrassinolide, 0.1kg penicillin, 0.4kg triacontanol and 0.4kg ethephon, finally add 11kg apple juice, stir and mix evenly to form the final product.

Embodiment 2

[0022] Put 49.7kg of water into the 100L reactor, then put in 1kg of ferric sulfate, 1kg of ethylenediaminetetraacetic acid disodium salt, 1kg of boric acid, 1kg of D-pantothenic acid, 1kg of copper sulfate, 1kg of ammonium nitrate, 1kg of crystalline magnesium sulfate, 1kg of sulfurous acid Iron, 1kg manganese sulfate, 1kg zinc sulfate, 2kg molybdenum trioxide and 2kg titanium dioxide, after stirring and dissolving, slowly drop into 2kg pyridoxine hydrochloride, 2kg glycine, 2kg folic acid, 2kg riboflavin, 2kg ascorbic acid, 0.5kg citric acid and 0.5kg of arginine, stir and mix evenly, then add 0.5kg of transzeatin, 0.5kg of cyberon, 0.5kg of α-naphthalene acetic acid, 0.5kg of 2,4-dichlorophenoxyacetic acid, 0.5kg of gibberellin, 0.5kg of epitope Brassinosteroids, 0.5kg cyanin, 0.5kg triacontanol and 0.3kg ethephon, finally add 10kg apple juice and 11kg tomato juice, stir and mix evenly to form the final product.

Embodiment 3

[0024] Put 69kg of water into the 100L reactor, then put in 0.5kg of ferric sulfate, 0.5kg of ethylenediaminetetraacetic acid disodium salt, 1kg of boric acid, 1kg of calcium chloride, 1kg of potassium iodide, 1kg of nitric acid, 1kg of crystalline magnesium sulfate, and 1kg of ferrous sulfate , 1kg manganese sulfate, 1kg zinc sulfate, 0.5kg molybdenum trioxide and 0.5kg titanium dioxide, stir and dissolve, then slowly add 0.5kg niacin, 0.5kg inositol, 0.5kg pyridoxine hydrochloride, 0.5kg glycine, 0.5kg folic acid , 0.5kg riboflavin, 0.5kg ascorbic acid, 0.5kg citric acid, 0.5kg arginine and 1.5kg peptone, stir and mix evenly, then add 0.2kg 3-indoleacetic acid, 0.2kg6-furfuryl aminopurine, 0.2kg chlorine Phenylfenuron, 0.2kg 2,4-dichlorophenoxyacetic acid, 0.2kg gibberellin, 0.25kg epibrassinolide, 0.25kg penicillin, 0.25kg triacontanol and 0.25kg ethephon, finally add 13kg sweet potato powder, stir and mix evenly to form the final product.

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Abstract

The invention provides a culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile, belonging to the technical field of plant cultivation. The culture medium comprises following substances according to weight percentages: 45%-80% of water, 3%-17% of an inorganic matter, 2%-15% of an organic matter, 0.1-7% of a plant growth regulator and 5%-30% of a synergic component. According to the culture medium for rapidly inducing the adventitious buds in the tissue culture of the dendrobium nobile, the requirements on nutritional ingredients caused by the dendrobium nobile in the tissue culture process and growth and development characteristics of the dendrobium nobile are utilized and the prepared culture medium can be used for obviously promoting callus of the dendrobium nobile to differentiate the adventitious buds; meanwhile, the rooting rate and the rooting quantity of the adventitious buds can be increased; the culture medium has the advantages of low production cost, convenience in utilization, safety in storage and transportation, high application and economic values, stability and high efficiency, rapidness in root developing and the like.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium dendrobium. Background technique [0002] Spring Dendrobium (Dendrobium) is a perennial herb of the Orchidaceae family (Orchidaceae). It has the advantages of rich colors, fresh fragrance, long ornamental period, and extensive management. Get the deep attention of practitioners. In the 1990s, spring dendrobium produced in South Korea, Japan and other countries began to enter the Chinese market. Due to the high price and the lack of localization technology in time, the spring dendrobium in the Chinese New Year flower market is currently at a high price, and the supply exceeds demand. It is very urgent to increase the research on the production technology of Dendrobium dendrobium seedlings, which has broad market prospects. [0003] According to relevant information, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李兴彪
Owner TIANMU COLLEGE OF ZHEJIANG A&F UNIV
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