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Method for producing yeast extract by use of bacterial residues after tetracycline degradation

A technology of yeast extract and tetracycline, applied in the field of microbial fermentation, to achieve high product quality, high value conversion, simple and unique preparation process

Inactive Publication Date: 2014-02-05
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for producing yeast extract by using the bacteria residue after tetracycline degradation treatment

Method used

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  • Method for producing yeast extract by use of bacterial residues after tetracycline degradation
  • Method for producing yeast extract by use of bacterial residues after tetracycline degradation

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Effect test

Embodiment 1

[0039] A method for producing yeast extract by utilizing the bacterium residue after degradation treatment of tetracycline, comprising the steps of:

[0040] S1: Preparation of secondary seeds of Saccharomyces cerevisiae, Candida tropicalis and EM protosol:

[0041] a. Seed Activation Cultivation: Pick a ring from the storage tubes of Saccharomyces cerevisiae, Candida tropicalis, and EM original dew, respectively, and insert them into the seed activation expansion medium, and culture them aerobically at 30°C and 100r / min for 48h. Obtain activated Saccharomyces cerevisiae, Candida tropicalis and EM raw dew seeds respectively; Described seed concentration is 10 7 CFU / mL;

[0042] b. First-level seed preparation: Inoculate the Saccharomyces cerevisiae, Candida tropicalis and EM raw dew seeds prepared in the above step a with an inoculation amount of 1% by mass percentage, respectively, inoculate the seed activation expansion medium at 30°C, 100r / After 48 hours of aerobic cultu...

Embodiment 2

[0058] A method for producing yeast extract by utilizing the bacterium residue after degradation treatment of tetracycline, comprising the steps of:

[0059] S1: Preparation of secondary seeds of Saccharomyces cerevisiae, Candida tropicalis and EM protosol:

[0060] a. Seed activation culture: Pick a ring from the storage tubes of Saccharomyces cerevisiae, Candida tropicalis, and EM original dew, respectively, and insert them into the seed activation expansion medium, and culture aerobically at 33°C and 120r / min for 42h. Obtain activated Saccharomyces cerevisiae, Candida tropicalis and EM raw dew seeds respectively; Described seed concentration is 10 7 CFU / mL;

[0061] b. First-level seed preparation: inoculate the Saccharomyces cerevisiae, Candida tropicalis and EM raw dew seeds prepared in the above step a with an inoculation amount of 3% by mass percentage, respectively, inoculate the seed activation expansion medium at 33°C, 120r / m After 42 hours of aerobic culture under...

Embodiment 3

[0077] A method for producing yeast extract by utilizing the bacterium residue after degradation treatment of tetracycline, comprising the steps of:

[0078] S1: Preparation of secondary seeds of Saccharomyces cerevisiae, Candida tropicalis and EM protosol:

[0079] a. Seed activation culture: Pick a ring from the storage tubes of Saccharomyces cerevisiae, Candida tropicalis, and EM original dew, respectively, and insert them into the seed activation expansion medium, and cultivate them aerobically at 35°C and 140r / min for 36h. Obtain activated Saccharomyces cerevisiae, Candida tropicalis and EM raw dew seeds respectively; Described seed concentration is 10 7 CFU / mL;

[0080] b. Primary seed preparation: Inoculate the Saccharomyces cerevisiae, Candida tropicalis and EM protozoa seeds prepared in the above step a with an inoculation amount of 5% by mass percentage, respectively, inoculate the seed activation expansion medium at 35°C, 140r / m After aerobic cultivation for 36 ho...

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Abstract

The invention discloses a method for producing a yeast extract by use of bacterial residues after tetracycline degradation, belonging to the technical field of microbial fermentation. The method comprises preparation of saccharomyces cerevisiae, candida tropicalis and EM (effective microorganisms) second-order seed, preparation of aspergillus niger seed, preparation of a bacterial residue culture medium and preparation of the yeast extract, wherein the preparation of the yeast extract comprises the following steps of performing inoculation culture on the prepared several kinds of bacteria; then adding a yeast extraction enzyme according to an amount of 0.03% by mass; hydrolyzing for 16-24 hours; heating to 80 DEG C to inactivate the enzyme; after the product is cooled, centrifuging for 10 minutes at a speed of 4,000r / min; performing reduced-pressure concentration on the filtrate while controlling the moisture to 15-20% to obtain the yeast extract. The method disclosed by the invention has the advantages of low production cost, simple and unique preparation process and short production period, and can realize high-value conversion of tetracycline bacterial residues.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing yeast extract by using fungus residue after tetracycline degradation treatment. Background technique [0002] Yeast extract is made from yeast, which is obtained by using modern biotechnology to hydrolyze the yeast cytoplasm or hydrolyze with enzyme preparations through the autolysis of yeast cells and the yeast's own enzyme system, and then undergo a series of extraction, concentration and other processes. The powder or paste or liquid products are rich in amino acids, functional polypeptides, anti-aging active factors, dietary fiber and mannose, and also contain indispensable nucleic acids, nucleotides and calcium for the human body, and are rich in B vitamins And phosphorus, magnesium, manganese, zinc, selenium and other trace elements. Yeast extract is widely used in biological fermentation, bioengineering, biomedicine and other fields. Specifical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/865C12R1/74
Inventor 马玉龙马凌王敏马琳
Owner NINGXIA UNIVERSITY
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