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Application of a Morinda officinalis oligosaccharide 6 saccharide in the preparation of a drug against myocardial ischemia and reperfusion injury

A Morinda officinalis oligosaccharide, anti-myocardial ischemia technology, applied in the field of medicinal application of plant extracts, can solve the problems of aggravating ischemic myocardial injury, intramyocardial hemorrhage and the like, and achieves stable properties, controllable quality and simple extraction methods. Effect

Active Publication Date: 2017-08-01
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Thrombolysis, coronary artery bypass grafting, and coronary intervention are effective methods for the treatment of acute myocardial infarction. These methods can recanalize the blocked coronary artery, but timely reperfusion of myocardial ischemia can save some damaged but not yet necrotic myocardium. cells, may also aggravate ischemic myocardium damage, manifested as accelerated necrosis of myocardial cells, cell swelling, formation of muscle fiber contractile bands, intramyocardial hemorrhage and no perfusion, and severe reperfusion arrhythmias may also occur

Method used

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  • Application of a Morinda officinalis oligosaccharide 6 saccharide in the preparation of a drug against myocardial ischemia and reperfusion injury
  • Application of a Morinda officinalis oligosaccharide 6 saccharide in the preparation of a drug against myocardial ischemia and reperfusion injury
  • Application of a Morinda officinalis oligosaccharide 6 saccharide in the preparation of a drug against myocardial ischemia and reperfusion injury

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of Morinda officinalis oligosaccharide 4, 5, 6 polysaccharide monomer:

[0048] (1) Dissolve 10g of Morinda officinalis extract in 80ml of H 2 O, and freeze-dried.

[0049] (2) Add 100ml of pyridine to 8.3g of freeze-dried product, and add 100ml of anhydrous acetic acid while stirring at a temperature of 0°C.

[0050] (3) After stirring for 24 hours in a greenhouse state, the excess medium was removed by decompression.

[0051] ⑷ add CHCl to the remaining part 3 , washed with saturated soda water and saturated saline.

[0052] (5) After drying with magnesium sulfate, remove CHCl under reduced pressure 3 .

[0053] (6) Separately purify by compound purification method (developing solvent: toluene: ethyl acetate (Toluene: Ethylacetate) = 2:1 → 1:1 → 1:2 → 1:3 → 1:5). 623 mg of acetylated Hex4 monomer (Hex4perAc) was extracted. 623 mg of acetylated Hex5 monomer (Hex5perAc) was extracted. 74 mg of acetylated Hex6 monomer (Hex6perAc) was extracted.

[00...

Embodiment 2

[0064] (1) Isolated endothelial cells and whole animal experiments

[0065] In the experimental system of neonatal rat cardiomyocytes, newborn (1-3d) SD / Wistar rats were used, and the heart was taken as primary cardiomyocyte culture, which was divided into normal control group, hypoxia-reoxygenation injury (H / R) model group, H / R+ positive drug (Di'ao Xinxuekang, 0.7g L -1 ) control group, H / R+Hex4 (42nmol·ml -1 ), Hex5 (30nmol·ml -1 ), Hex6 (22nmol·ml -1 )each group. The cardiomyocyte morphology and cardiomyocyte ultrastructure were used as the main evaluation indexes to observe the protective effect of Morinda officinalis 4, 5, and 6 glycans on cardiomyocytes.

[0066] In the experimental system of human umbilical vein endothelial cell (HUVEC) angiogenesis model, it was divided into normal control group, H / R model group, H / R+positive drug (Shexiang Baoxin Pill, 2g·L -1 ) control group, H / R+Hex4 (42nmol·ml -1 ), Hex5 (30nmol·ml -1 ), Hex6 (22nmol·ml -1 )each group. ...

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Abstract

The invention discloses an application of a Morindae officinalis extract product and Morindae officinalis oligosaccharide 6 glycan in the preparation of medicines for resisting myocardial ischemia and / or reperfusion injury and promoting therapeutic angiogenesis. The myocardial ischemia and reperfusion injury are body injuries caused by the afresh circulation establishment and the blood supply recovery during the stopping or abnormality of the myocardial blood perfusion, like ischemic and anoxic injuries. The Morindae officinalis oligosaccharide 6 glycan has a protect effect on an in-vivo rat myocardial ischemia reperfusion injury model and an in-vitro purify-cultured mouse myocardial cell reoxygenation injury model, can substantially reduces the myocardial infarction area, can mitigate the reperfusion arrhythmia, can effectively protect the form of myocardial cells, and can mitigate the injuries of anoxic reoxygenation to the myocardial cells. The therapeutic angiogenesis promotion is characterized in that the ischemic myocardial blood supply recovery and the cardiac function improvement are realized by increasing the functional coronary arterial tree or coronary collateral through some irradiations comprising the treatment effects of some medicines.

Description

technical field [0001] The invention relates to the field of medicinal applications of plant extracts, in particular to the application of Morinda officinalis oligosaccharides in the preparation of anti-myocardial ischemia and reperfusion injury drugs. Background technique [0002] Coronary atherosclerotic heart disease (coronary heart disease, CAD), referred to as coronary heart disease, also known as ischemic heart disease, is a common cardiovascular disease in humans and the most important fatal cardiovascular disease in adults. Acute myocardial infarction caused by coronary heart disease is the main cause of high morbidity and mortality of cardiovascular diseases. The incidence of coronary heart disease continues to increase, and the younger trend is obvious. Coronary heart disease is clinically manifested as severe myocardial ischemia, which seriously threatens human health. Mortality and long-term prognosis in patients with coronary heart disease are related to left ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61K31/702A61P9/10A61P9/00C07H1/08C07H3/06
Inventor 冯国清姚闵胡香杰赵胜张贺鸣杨景柯王奎鹏于爽察雪湘孟祥光吴瑶焦平利王宁刘茜徐君玲张晓宁郭婷婷王涵澄
Owner ZHENGZHOU UNIV