Dithiothreitol Membrane Mercury Ion Selective Electrode Method
A dithiothreitol and selective electrode technology, applied in the field of chemical/biological sensing, to achieve the effects of easy surface modification, high flatness and high sensitivity
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Embodiment 1
[0018] Assembly preparation of selected electrodes:
[0019] A schematic diagram of the planar structure of a dithiothreitol membrane mercury ion selective electrode figure 2 As shown, the selection electrode includes a substrate 11 of a working gold film 12 modified by a dithiothreitol film layer 25, and the working gold film 12 covered on the substrate 11 is connected to the pin gold film 13 through the gold film layer, Pin gold film 13 is connected with the metal wire lead 15 that is covered with plastic insulation layer 14 by soldering tin 16, and the material of metal wire lead 15 is copper wire, and the junction is wrapped and sealed with epoxy resin glue 17, to guarantee good conduction, Continuity.
[0020] Such as figure 2 As shown, the profile design of the substrate 11 is as follows: the shape of the working gold film 12 covered on the substrate 11 is a square, and the side length is 6mm; the shape of the pin gold film 13 is a small rectangle, the length is 3mm,...
Embodiment 2
[0024] Detection of mercury ions:
[0025] The mercury ion selective electrode 1 modified by the dithiothreitol film layer 25 modified by the above method is used as the working electrode, and the saturated calomel electrode 2 is used as the reference electrode, and the two electrodes are connected to the potentiometer 3 through wires to perform electrochemical potential Test; during test, dithiothreitol membrane mercury ion selective electrode 1 and saturated calomel electrode 2 are inserted in the beaker 5 that stirring magnet 4 is housed, and beaker 5 is placed on the magnetic stirrer 6, and mercury-containing The pH buffer solution of ions; by testing the potential value of a series of mercury ion standard solutions with known concentrations, the working curve is made with the potential value against the concentration value, and then the potential value of the unknown concentration mercury ion sample solution is tested, and the sample is obtained through the working curve ...
Embodiment 3
[0027] Reproducibility of selected electrodes:
[0028] The mercury ion selective electrode 1 modified by 10 dithiothreitol film layers 25 modified by the method described above was used as the working electrode, and the saturated calomel electrode 2 was used as the reference electrode, respectively for 1.0 × 10 -6 mol / L and 1.0×10 -7 The mol / L mercury ion solution sample was tested for electrochemical potential. It can be seen that after 10 consecutive measurements, the relative standard deviations were 2.4% and 3.1%, respectively, indicating that the selective electrode detection method has good reproducibility.
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