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Vascularizing tissue structure with microfluid passage and preparation method thereof

A technology of microfluidic channels and tissue structures, applied in biochemical equipment and methods, devices of human tubular structures, blood vessels, etc., can solve problems such as the inability to prepare vascularized tissue and organ structures, and achieve the convenience of nutrient exchange and cell attachment Attached, it facilitates the exchange of nutrients and maintains a stable effect

Inactive Publication Date: 2014-05-07
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing rapid prototyping and organ manufacturing technologies cannot prepare vascularized tissue and organ structures with microvascular systems that can be directly connected to human arteriovenous vessels

Method used

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  • Vascularizing tissue structure with microfluid passage and preparation method thereof
  • Vascularizing tissue structure with microfluid passage and preparation method thereof
  • Vascularizing tissue structure with microfluid passage and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0041] A method for preparing a vascularized tissue structure with a microfluidic channel provided by the present invention is characterized in that the method comprises the following steps: 1) using a computer to design a three-dimensional model of the vascularized tissue structure with a microfluidic channel; 2) Composite multi-nozzle rapid prototyping equipment is adopted, and the temperature of the forming room is controlled at 40°C to -30°C; the nozzle components of the equipment include extrusion nozzles, drip nozzles and spray nozzles; the prepared natural polymer water containing cells The gel, the dilute solution containing or not containing cells and the synthetic polymer solution that can maintain cell survival are respectively loaded into different types of nozzle assemblies; 3) using extrusion nozzle assemblies to print at least one layer of natural polymer hydrogel or Synthesizing a polymer solution to obtain a supporting part; 4) Printing at least one layer of th...

Embodiment 1

[0045] Example 1: Preparation of vascularized liver tissue with microfluidic channels

[0046] (1) Cell extraction: human adipose stem cells (ADSC) and hepatocytes (Hep) were extracted, cultured and passaged.

[0047] (2) Preparation of hydrogel: Dissolve gelatin and sodium alginate powder in culture medium (DMEM, dulbecco's modified eagle medium) respectively to obtain a gelatin solution with a mass volume concentration of 10% and a mass volume concentration of 2% The sodium alginate solution is mixed with the above gelatin solution, sodium alginate solution equal volumes to obtain hydrogel stand-by.

[0048] (3) Preparation of synthetic polymer solution: the degradable polycarbonate was dissolved in tetraethylene glycol to obtain a solution with a mass volume concentration of 2% and a mass volume concentration of 25% for use.

[0049](4) Preparation of cell-containing matrix material: mix hepatocytes (Hep), dimethyl sulfoxide cryopreservation agent and the above-mentioned h...

Embodiment 2

[0052] Example 2: Preparation of Vascularized Adipose Tissue with Microfluidic Channels

[0053] (1) Cell extraction: human adipose stem cells (ADSC) were extracted, cultured and passaged.

[0054] (2) Preparation of hydrogel: Dissolve the natural polymer material powder in DMEM culture fluid to obtain a gelatin solution with a mass volume concentration of 20% and a fibrinogen solution with a mass volume concentration of 1%. Equal volumes of the protein solution were mixed to obtain a hydrogel material solution for use.

[0055] (3) Preparation of synthetic polymer solutions: PLGA was dissolved in tetraethylene glycol to obtain solutions with mass volume concentrations of 2% and 15% for use.

[0056] (4) Preparation of cell-containing material: mix adipose stem cells (ADSC), glycerol cryopreservation agent and the above-mentioned hydrogel material solution, and the ADSC concentration of the hydrogel solution is 5×10 6 cells / mL, the volume concentration of glycerol cryopreser...

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Abstract

The invention relates to a vascularizing tissue structure with a microfluid passage and a preparation method thereof, and belongs to the technical field of tissue engineering and forming of composite material. The vascularizing tissue structure comprises a tissue structure body, branch blood vessel parts, a capillary layer and a protecting layer. The vascularizing tissue structure has the advantages that the composite forming of thin solution, cells and water gel is realized; the tissue structure body is formed by spraying the water gel containing the cells, and the thin solution containing or not containing the cells is distributed among the grids of the water gel; a branch blood vessel hole passage is reserved by the water gel containing the cells, the thin solution containing or not containing the cells is distributed in the hole passage, and the wall of each branch blood vessel is a porous structure; the capillary layer is formed by coating or spraying the polymer thin solution, or the thin solution containing the cells; the support part and the protecting part are formed by stacking synthetic or natural polymer solution; the two ends of the formed structure body are provided with blood vessel joints or a plurality of opened type passages, and can be used for in-vivo implanting or in-vitro culturing, so as to promote the development of vascularizing.

Description

technical field [0001] The invention relates to a vascularized tissue structure with a microfluidic channel and a preparation method thereof, and belongs to the technical field of tissue engineering and composite material forming. Background technique [0002] Tissue engineering technology and organ manufacturing technology provide the possibility for the repair and reconstruction of human tissues and organs, which involves biology, materials science, mechanics and other disciplines. At present, it has been possible to regenerate bone, cartilage, skin, liver, muscle, and other tissue or organ precursors, but the technology of organ manufacturing is still in the development stage, and it is urgent to solve the problem of vascularization, because the normal metabolism of blood vessels in organs is an organ. premise of manufacture. [0003] Rapid prototyping (RP, Rapid Prototyping) technology is also called additive manufacturing (AM, Additive Manufacturing), which uses the la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06A61L27/44A61L27/38A61L27/52
CPCA61L27/52A61F2/062A61L27/18A61L27/3808A61L27/507A61L27/56C12M21/08C12M21/14C08L75/04C08L67/04
Inventor 王小红许雨帆
Owner TSINGHUA UNIV
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