Culture medium and culture method for culturing haematococcus pluvialis by using brewery wastewater

Abstract

The invention relates to a culture medium for culturing haematococcus pluvialis by using brewery wastewater, of which the formula is as follows: 80 ml of brewery wastewater, 28 mg of sodium citrate, 20 mg of urea, 12 mg of potassium dihydrogen phosphate, 150 mg of fructose, 45 mg of yeast extract powder, 50 mg of casein tryptone, 12 mg of ammonium citrate, 25 mg of whey powder, 30 mg of lactose, 60 mg of glucose, 1.8 ml of horse manure leachate, 2 ml of fermented soya-bean milk, 100 mg of sorbitol, 24 mg of soluble starch, 25 mg of soytone, 28 mg of beta-sodium glycerophosphate, 12 mg of an edetic acid, 10 mcg of vitamin B1, 21 mcg of vitamin B1, 1.8 ml of soil extract, 1.5 ml of human urine, 2.4 ml of chicken manure leachate, and 1000 ml of pure fresh water disinfected by sodium hypochlorite. An important bioactive substance-astaxanthin can be obtained. The culture medium is low in cost, uneasily polluted, and can be reused; and due to the mixed utilization of organic fertilizers and inorganic fertilizers, the growth rate and astaxanthin content of haematococcus pluvialis are significantly increased, and the yield is increased by 270%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a culture medium and a culture method for cultivating Haematococcus pluvialis by using wastewater from a brewery. Background technique [0002] The wastewater produced in the beer brewing process has the characteristics of large amount of wastewater, high content of suspended solids and organic matter. Wastewater itself is not toxic, but a large amount of biodegradable organic matter contained in it is directly discharged into the water body, which will consume a large amount of dissolved oxygen in the water, resulting in hypoxia in the water body, resulting in the death of aquatic organisms including fish, and the waste water contains a large amount of N and P inorganic substances. Salt causes eutrophication of water bodies, deteriorates water quality, and pollutes the environment. Beer wastewater is divided into three parts: one is cooling water, accounting for 70% of th...

AI Technical Summary

Problems solved by technology

In short, UASB has the advantages of high efficiency, low cost, low power consumption, less investment, and less land occupation, but the disadvantage is that the concentration of CODcr in the effluent is still high, reaching 500mg / L

[0008] The cultivation of Haematococcus pluvialis traditionally uses BBM medium and open cement ponds, which has slow growth, low yield, low astaxanthin content, large investment, easy pollution, and low benefit

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