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Biomacromolecule/chitin nanocrystalline composite scaffold material and preparation method thereof

A technology of chitin nanocrystals and biopolymers, which is applied in the field of biomedical materials and tissue engineering, can solve the problems of mechanical properties that need to be improved, lack of biological activity, and cells that cannot adhere, so as to facilitate reconstruction and repair and promote adhesion. and mineralization, enhance the effect of biological activity

Active Publication Date: 2014-08-27
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current biodegradable bone tissue scaffolds such as poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV), polylactic acid (PLA), and polycaprolactone (PCL) porous scaffolds have poor hydrophilicity and do not have Biological activity, cells cannot adhere well on the scaffold; and the mechanical properties need to be improved

Method used

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  • Biomacromolecule/chitin nanocrystalline composite scaffold material and preparation method thereof
  • Biomacromolecule/chitin nanocrystalline composite scaffold material and preparation method thereof
  • Biomacromolecule/chitin nanocrystalline composite scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step (1). Weigh 2g of PHBV powder with a weight-average molecular weight of 250,000 and a molar content of hydroxyvalerate (HV﹪) of 20%, dissolve it in 10ml of dioxane, and stir magnetically at 90°C for 2 hours to form a PHBV solution . Ultrasonic disperse 0.1g of chitin nanocrystals in 10ml of dioxane, and disperse evenly to obtain a chitin nanocrystal solution; then add the dispersed chitin nanocrystal solution to the PHBV solution, stir magnetically for 30 minutes, and then pour in a cylindrical mold.

[0028] Step (2). Weigh 6 g of sodium chloride particles sieved by a standard sieve with a particle size ranging from 200 to 350 μm, and add to the above mixed solution. Place the mold on a vortex mixer to mix evenly, and freeze in a -20°C refrigerator for 4 hours.

[0029] Step (3). Take the frozen and solidified PHBV / chitin nanocrystal mixture out of the mold, put it into 500ml of ice water, change the distilled water every 4 hours, and stir it magnetically for 2 d...

Embodiment 2

[0034] Step (1). Weigh 1 g of polylactic acid with a weight average molecular weight of 200,000, dissolve it in 10 ml of dioxane, and stir magnetically at 90°C for 2 hours to form a uniform polylactic acid solution. Ultrasonic disperse 0.1g of chitin nanocrystals in 5ml of dioxane, and disperse evenly to obtain a chitin nanocrystal solution; then add the dispersed chitin nanocrystals to the polylactic acid solution, stir magnetically for 30 minutes, and pour into in a cylindrical mold.

[0035] Step (2). Weigh 9 g of sodium potassium tartrate tetrahydrate granules with a particle size ranging from 200 to 400 μm and sieve through a standard sieve, and add them to the above mixed solution. Place the mold on a vortex mixer to mix evenly, and freeze in a -20°C refrigerator for 4 hours.

[0036] Step (3). Take the frozen and solidified polylactic acid / chitin nanocrystalline mixture out of the mold, put it into 500ml ice water, change the distilled water every 4 hours, and stir it ...

Embodiment 3

[0039] Step (1). Weigh 2 g of polycaprolactone with a weight average molecular weight of 100,000 and dissolve it in 20 ml of dichloromethane / dioxane mixed solution (the volume ratio of dichloromethane to dioxane is 2:8) , 40 ° C magnetic stirring for 2 hours to form a homogeneous polycaprolactone solution. Ultrasonic disperse 0.2g of chitin nanocrystals in 10ml of dichloromethane / dioxane mixed solution, and disperse evenly to obtain a chitin nanocrystal solution; then add the dispersed chitin nanocrystals to the polycaprolactone solution , stirred magnetically for 30 minutes and poured into cylindrical molds.

[0040] Step (2). Weigh 6 g of sodium chloride particles sieved with a standard sieve in the particle size range of 200-300 μm, and add to the above mixed solution. Place the mold on a vortex mixer to mix evenly, and put it in a -80°C refrigerator for 4 hours.

[0041] Step (3). Take out the frozen and solidified polycaprolactone / chitin nanocrystalline mixture from the...

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Abstract

The invention discloses a biomacromolecule / chitin nanocrystalline composite scaffold material and a preparation method thereof. Being a blend, the biomacromolecule / chitin nanocrystalline composite scaffold material comprises a biodegradable macromolecule and chitin nanocrystalline in a mass ratio of 1:10-1:100. The biodegradable macromolecule / chitin nanocrystalline composite bone tissue scaffold material is prepared by a freezing / pore-foaming agent leaching method. The composite scaffold has an average porosity of greater than 85%, the pore diameter is regulatable, and the compression modulus is 2MPa-12MPa. By introducing chitin nanocrystalline into a biomacromolecule porous scaffold, the mechanical strength and bioactivity of the scaffold are improved, and the adhesion of cells on the scaffold and the mineralization activity are promoted. The biomacromolecule / chitin nanocrystalline composite scaffold can be applied in the bone repair field as a bone tissue engineering scaffold.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and tissue engineering, and relates to a biopolymer / chitin nanocrystal composite support material and a preparation method thereof. Background technique [0002] The purpose of tissue engineering is to research and develop tissue or organ substitutes for repairing and maintaining damaged human body. Tissue engineering basically consists of four parts: cell isolation and culture, construction of cell culture scaffolds, in vitro culture of cell / scaffold complexes, and transplantation. Among them, cell culture scaffold materials play an important role in supporting cell growth, guiding tissue regeneration, controlling tissue structure and releasing bioactive factors. [0003] The cell culture scaffolds used in tissue engineering must meet the following requirements: (1) have good biocompatibility, (2) have certain mechanical properties, (3) have appropriate porosity and pore size to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/26A61L27/56A61L27/54A61L27/50
Inventor 李湖燕蒋志强李赫曾志翔乌学东
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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