Coreopsis tinctoria flower polysaccharide oral liquid and production method thereof

A technology of oral liquid and snow chrysanthemum, which is applied in the direction of medical formula, anti-toxic agent, antibacterial drug, etc., can solve the problems of no research and analysis of snow chrysanthemum polysaccharide oral liquid, and achieve anti-oxidative body immunity, high purity, and obvious antibacterial The effect of action

Active Publication Date: 2014-09-17
URUMQI SHENGMING HELI HIGH TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the current situation that there is no research and analysis on functional snow chrysanthemum polysaccharide oral liquid
The purpose of the present invention is to adopt biocomposite enzymolysis technology and ultrasonic technology to extract and adopt polyamide column chromatography technology to purify snow chrysanthemum polysaccharide, the method for filling, sterilizing, packing to produce snow chrysanthemum polysaccharide oral liquid, and this product is cured The evaluation not only solves the current problem of only analyzing the potency of a single component, but also provides a practical method for snow chrysanthemum processing enterprises to improve their technical level

Method used

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  • Coreopsis tinctoria flower polysaccharide oral liquid and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of Xueju Polysaccharide Oral Liquid

[0030] See attached figure 1 , snow chrysanthemum polysaccharide oral liquid is prepared through the following specific steps:

[0031] (1) Select dried snow chrysanthemums with high uniformity, full flower bones, orange-yellow stamens and petals, no leaf stalks, fine stamens or other non-snow chrysanthemum impurities, and crush the selected snow chrysanthemums. The particle size of snow chrysanthemum powder is 100 mesh.

[0032] (2) Snow chrysanthemum powder and water according to the mass volume ratio of 1:30, add water to the snow chrysanthemum powder in step (1), stir evenly with a glass rod, put it in a constant temperature water bath device to preheat for half an hour, The constant temperature water bath temperature is 55°C.

[0033] (3) Ultrasonic treatment was performed on the solution treated in the above step (2). The temperature during the ultrasonic treatment was 55°C, the time was 15 minutes,...

Embodiment 2

[0043] Example 2: Preparation of Xueju Polysaccharide Oral Liquid

[0044] In order to further illustrate the present invention, take 5g snow chrysanthemum as example.

[0045] (1) Select 5g of dried snow chrysanthemums with high uniformity, full flower bones, orange-yellow stamens and petals, no leaf stems, fine stamens or other non-snow chrysanthemum impurities, and crush the selected 5g snow chrysanthemums in a universal Crushed in the machine, the particle size of the pulverized snow chrysanthemum powder is about 100 mesh.

[0046] (2) Add 150ml of distilled water to the crushed snow chrysanthemum powder, stir evenly with a glass rod, and put it into a constant temperature water bath heated to 55°C to preheat for half an hour.

[0047] (3) The solution treated in the above step (2) was subjected to ultrasonic treatment with an ultrasonic breaker, the temperature during ultrasonic treatment was 55°C, the time was 15 minutes, and the power was 160w.

[0048] (4) Pour the...

Embodiment 3

[0054] Example 3: Potency analysis experiment of snow chrysanthemum polysaccharide oral liquid

[0055] Xueju polysaccharide oral liquid has antibacterial effect:

[0056] Spread Staphylococcus aureus evenly on the plate medium filled with beef extract peptone on the aseptic operating table, divide the plate into four parts, and soak the snow chrysanthemum prepared in the above examples at a pair of diagonal positions The filter paper sheet of the polysaccharide oral liquid is pasted on the flat plate, and the filter paper sheet immersed in sterile physiological saline is pasted on the flat plate at the other diagonal position. Do three parallels. The plates were incubated in a constant temperature incubator at 37°C for 24h. By observing and measuring the diameter of the inhibition zone to compare the size of the antibacterial effect. The experimental results show that the Xueju polysaccharide oral liquid has a significant effect on inhibiting the growth of Staphylococc...

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Abstract

The invention discloses a method for preparing coreopsis tinctoria flower polysaccharide oral liquid. The method comprises the following steps: mixing the raw material coreopsis tinctoria flower powder with water uniformly at a massic volume ratio of 1:30; after carrying out thermostatic waterbath, carrying out ultrasonic treatment, wherein the temperature for ultrasonic treatment is 55DEG C, the time for ultrasonic treatment is 15minutes, and the power is 160w; carrying out enzymolysis on the coreopsis tinctoria flower solution, wherein the dosage of cellulase for enzymolysis is 1%, the dosage of pectinase is 1%, the dosage of papain is 1.5%, the enzymatic time is 3 hours, and the temperature is 55DEG C; deactivating the enzyme and centrifuging to obtain supernate, and carrying out chromatography by use of a polyamide column; blending the effluent liquid with distilled water, wherein the weight/volume ratio of the effluent liquid and distilled water is 1:0.8; bottling the blended solution, sterilizing, and packaging so as to obtain the coreopsis tinctoria flower polysaccharide oral liquid. The oral liquid has obvious antibacterial activity, can be used for clearing and reducing free radical 1,1-diphenyl-2-picrylbydrazyl (DPPH) and hydroxyl radical, has the effect of improving the organism immunity sharply, and has extensive application value.

Description

technical field [0001] The invention relates to the technical field of preparation of snow chrysanthemum polysaccharide oral liquid. Specifically, the invention relates to the technical field of producing and preparing snow chrysanthemum polysaccharide oral liquid with antibacterial, anti-oxidation and improving human immunity. Background technique [0002] Snow chrysanthemum grows in the Kunlun Mountains above 2,600 meters above sea level. It is the only rare alpine plant in Xinjiang that is as famous as snow lotus and has unique effects. The petals are golden yellow, and the spherical stamens are brown. The highest-grade snow chrysanthemum refers to the Victoria's Secret Kunlun snow chrysanthemum that grows in the alpine region at an altitude of about 3,000 meters, and is called "high-altitude Kunlun snow chrysanthemum". At present, more than 20 categories and more than 300 kinds of natural ingredients have been isolated and identified from Snow Chrysanthemum, including ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/04A61P37/04A61P39/06
Inventor 陈安新郭媛媛王亮
Owner URUMQI SHENGMING HELI HIGH TECH
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