Method for preparing soil restoration modifier by utilization of spore fermentation wastewater

A technology of soil remediation and fermentation waste liquid, which is applied in the direction of soil conditioning materials, chemical instruments and methods, applications, etc., can solve undeveloped problems, achieve the effects of inhibiting breeding, low operating costs, and protecting the environment

Active Publication Date: 2014-09-17
七禾河(上海)生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it can be considered to use the spore fermentation waste li...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In the first step, the composite strains are inserted into the culture medium and cultured at 30° C. for 120 hours to obtain the composite strains.

[0034] Wherein, the composite bacterial species includes 0.5×10 9 cfu / ml Rhodopseudomonas palustris 5wt%, 5×10 9 cfu / ml Lactobacillus plantarum 35wt%, 5×10 9 cfu / ml Streptococcus lactis 40wt%, and 2.5×10 9 cfu / ml brewer's yeast 20wt%. The weight ratio of the composite strain to the culture medium is 1:99.

[0035] Wherein, the medium is composed of molasses 1wt%, peptone 0.4wt%, yeast extract 1wt%, calcium chloride 0.1wt%, sodium chloride 0.2wt%, iron sulfate 3.0wt%, magnesium sulfate 0.02wt%, growth factor solution 1wt% (including vitamin H2wt%, vitamin B12wt% aqueous solution), soil leachate 10wt% and the rest of water, pH6.5.

[0036] In the second step, the composite bacterial strain obtained in the first step is inoculated in the fermentation medium, mixed evenly, and anaerobically fermented for 72 hours at 33.3°...

Embodiment 2

[0043] In the first step, the composite strains are inserted into the culture medium and cultured at 37° C. for 130 hours to obtain the composite strains.

[0044] Wherein, the composite bacterial species includes 0.5×10 9 cfu / ml Rhodopseudomonas palustris 7wt%, 5×10 9 cfu / ml Lactobacillus plantarum 45wt%, 5×10 9 cfu / ml Streptococcus lactis 45wt%, and 2.5×10 9 Two or more of cfu / ml brewer's yeast 25wt%. The weight ratio of the composite strain to the culture medium is 2:98.

[0045] Wherein, the medium is composed of molasses 1.2wt%, peptone 0.6wt%, yeast extract 1.2wt%, calcium chloride 0.15wt%, sodium chloride 0.25wt%, iron sulfate 3.5wt%, magnesium sulfate 0.03wt%, growth Factor solution 1.2wt% (including vitamin H2.3wt%, vitamin B15wt% aqueous solution), soil leaching solution 13wt% and the rest of water, pH6.5.

[0046] In the second step, the mother liquor of the composite bacterial line obtained in the first step is inserted into the substrate carrier 30t%, spore f...

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Abstract

The invention provides a method for preparing a soil restoration modifier by the utilization of spore fermentation wastewater, comprising the following steps: inoculating a composite strain to a medium and carrying out anaerobic cultivation to obtain a composite microbial system; and inoculating the composite microbial system to a fermentation medium and carrying out anaerobic fermentation to obtain the soil restoration modifier. The soil restoration modifier contains a plurality of active microorganisms, enzyme, organic acid and antibiotics, can play a role in promoting a benign micro-ecological environment in crop soil, can be used to solubilize organic and inorganic nutrients, promote nutrient absorption and conversion of crops, improve granular structure of soil and simultaneously inhibit propagation of harmful pathogen. Pesticide residues in soil are decomposed to some extent. By the method, the purpose of emission reduction can be achieved, and the environment is protected. In addition, nutritional ingredients in wastewater can be fully used in the production of the soil restoration modifier. Cost and water resources are saved. The method is simple and easy to operate, has low operation cost and is easy for industrial application.

Description

technical field [0001] The invention relates to a method for preparing a soil remediation improver, in particular to a method for preparing a soil remediation improver by using spore fermentation waste liquid. Background technique [0002] Bacillus (Bacillaceae), a family of bacteria, bacilli or cocci that can form spores (endospores). Including Bacillus, Lactobacillus, Clostridium, Desulfurized Enterobacter and Bacillus sarcina, etc. They are highly resistant to external harmful factors, widely distributed, and exist in soil, water, air, and animal intestines. Bacillus A genus of bacteria in the Bacillus family. [0003] Studies have shown that Bacillus can produce a variety of digestive enzymes to help animals digest and absorb nutrients. Bacillus has strong protease, amylase and lipase activities, and also has enzymes that degrade complex carbohydrates in feed, such as pectinase, glucanase, cellulase, etc. These enzymes can destroy the cell wall of plant feed cells , ...

Claims

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Application Information

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IPC IPC(8): C09K17/40
Inventor 刘杨周文明
Owner 七禾河(上海)生态科技有限公司
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