Type I collagen-sodium alginate-polyvinyl alcohol composite film and preparation method thereof

Abstract

The invention provides an I-type collagen-sodium alginate-polyvinyl alcohol composite film which comprises the following components by weight percent: 20% to 60% of I-type collagen, 20% to 60% of sodium alginate and 20% to 60% of polyvinyl alcohol. A preparation method of the composite film comprises the following steps: firstly, respectively dissolving the I-type collagen, the sodium alginate and the polyvinyl alcohol in different reaction kettles and regulating the pH of the I-type collagen to be within 5 to 8; then, successively adding a sodium alginate liquid and a polyvinyl alcohol liquid in proportion into an I-type collagen liquid and stirring for 4 to 10 hours; adding a defoaming agent to a mixed liquid and stirring for 20 to 60 minutes; then, pumping the mixed liquid into a storage tank and standing for defoaming; after the defoaming is checked to reach a requirement, pumping the mixed liquid into a film casting machine so as to obtain a film in a casting manner, drying and soaking the film in a cross-linking agent liquid or standing under a cross-linking condition for cross-linking for 4 to 24 hours, cleaning and drying; finally, shaping the film, packaging and irradiating, thereby obtaining the I-type collagen-sodium alginate-polyvinyl alcohol composite film. The composite film material is good in mechanical property, hydrophilcity and biocompatibility, moderate in degradation velocity and simple in preparation process. Thus, the composite film material can be widely applied to wound dressing, hemostatic materials, tissue engineering scaffold materials and the like.

Description

technical field [0001] The invention relates to a type I collagen-sodium alginate-polyvinyl alcohol composite film and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] The special structure of the three polypeptide chains of type I collagen to form a triple helix determines some of its specific properties. Its tensile strength is high and it has excellent fibrogenesis properties, which can promote cell proliferation, growth, degradability, tissue compatibility, and hemostasis. Collagen type I is widely used in the field of tissue engineering because of its good properties such as sex and collagen self-association. Due to its good biodegradability and biocompatibility, sodium alginate has been widely used in the fields of chemistry, biology, medicine, food, etc., and is widely used as a film material for various purposes due to its good film-forming properties preparation. PVA has good solvent resistance and excelle...

AI Technical Summary

Problems solved by technology

In the above method, there are the following deficiencies: (1) Most of the collagen raw material (collagen) is a hydrolyzed product of collagen, which has lost the unique biological activity of collagen when it maintains a triple helix; (2) artificial polymer materials such as collagen and PLA, PLGA, etc. When compounding, it needs to be dissolved with an organic solvent, such as hexafluoroisopropanol or tetrahydrofuran and other highly toxic solvents, which will affect the structure and function of the collagen after dissolution, and the residual organic solvent will directly affect the biocompatibility of the material; ( 3) The membrane material of collagen and sodium alginate has poor mechanical properties and the structure is easy to collapse without cross-linking, so it is not suitable as a scaffold material