Streptomyces albus and application thereof

A technology of Streptomyces albicans and white, which is applied in the direction of bacteria, microorganisms, fermentation, etc., can solve the problems of Streptomyces albicans, such as high requirements for culture conditions, complicated separation and purification process, and low ε-PL yield, so as to achieve easy separation and purification, culture medium Simple ingredients, good antibacterial effect

Active Publication Date: 2014-10-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] It is known in the prior art that Streptomyces albicans can be used to produce ε-PL, but there are many defects that Streptomyc

Method used

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  • Streptomyces albus and application thereof
  • Streptomyces albus and application thereof
  • Streptomyces albus and application thereof

Examples

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Effect test

Embodiment 1

[0021] Embodiment 1. Isolation and preservation of Streptomyces albicans Ls2

[0022] Streptomyces albicans Ls2 of the present invention is separated and obtained by following steps:

[0023] (1) The enrichment medium was used to enrich and cultivate the microorganisms in the soil collected from Hangzhou. The culture temperature was 28-30° C. and the culture time was 6-7 days to obtain white actinomycetes with dry edges and collapsed centers.

[0024] The composition of the enrichment medium is calculated in parts by weight:

[0025] 20 parts of soluble starch, 0.5 parts of sodium chloride, 1 part of potassium nitrate, 0.5 parts of dipotassium hydrogen phosphate, 0.5 parts of magnesium sulfate, 0.01 parts of ferrous sulfate, 0.66 parts of ammonium sulfate, 0.01 parts of zinc sulfate, 0.1 parts of yeast extract, weight 0.05 parts of potassium chromate, 15 parts of agar powder, and 1000 parts of water.

[0026] The method for preparing the enrichment medium is: weigh soluble s...

Embodiment 2

[0033] Embodiment 2. Utilize Streptomyces albicans Ls2 to produce ε-poly L-lysine

[0034](1) Inoculate the test tube species of Streptomyces albicans Ls2 in the liquid shake flask fermentation medium, the culture temperature is 28-30° C., and the culture time is 2 days to obtain the fermentation liquid, without adjusting the pH value during the culture process;

[0035] The composition of the liquid shake flask fermentation medium is calculated in parts by weight:

[0036] 5 parts of yeast extract, 50 parts of glucose, 10 parts of ammonium sulfate, 0.03 parts of ferrous sulfate, 0.5 parts of magnesium sulfate, 0.04 parts of zinc sulfate, 0.8 parts of dipotassium hydrogen phosphate, 1.36 parts of potassium dihydrogen phosphate, and 1000 parts of water;

[0037] The method for preparing the liquid shake flask fermentation medium is as follows: weigh yeast extract, ammonium sulfate, ferrous sulfate, magnesium sulfate, zinc sulfate, dipotassium hydrogen phosphate, and potassium d...

Embodiment 3

[0041] Example 3. Purification and detection of ε-poly L-lysine

[0042] Dissolve the ε-poly L-lysine obtained in Example 2 in deionized water, and perform adsorption analysis with a G-250 column to obtain ε-poly L-lysine with a purity of more than 98%, with a yield of 0.42 g / L fermentation broth. According to SDS-PAGE electrophoresis, the molecular weight of the ε-poly-L-lysine is about 3000Da.

[0043] Get the epsilon-poly L-lysine of embodiment 2 gained and dissolve with deionized water, make the solution that concentration is 0.1 gram per liter, carry out the filter paper sheet bacteriostasis test, the result shows that it is to escherichia coli and Staphylococcus aureus all. have a significant inhibitory effect, such as Figure 3a and 3b shown.

[0044] After storing the ε-poly-L-lysine obtained in Example 2 in a refrigerator at 4° C. for one month, the molecular weight and antibacterial properties did not change.

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Abstract

The invention relates to a streptomyces albus Ls2 for producing epsilon-poly L-lysine. The preservation number of the streptomyces albus Ls2 is CGMCC NO.8472; a colony formed by propagating and growing single cells on a Gao shi No.1 solid culture medium is white, filiform, dried on the edge and recessed in the center; microscopic examination shows that the cell is bacilliform without obstruction, is 1.0-3.0mm long; the streptomyces albus Ls2 is gram-negative; the streptomyces albus Ls2 can grow at the temperature of 20-40 DEG C, and the optimum growth temperature is 28-30 DEG C; a growth pH range is 3.0-9.5, and the optimum growth pH is 6.8-7.2; the streptomyces albus Ls2 is of oxybiontic growth; orange red precipitations are generated through a bismuth potassium iodide experiment, and orange precipitations are generated through a methyl orange experiment. The invention also relates to application of the streptomyces albus Ls2 in preparation of the epsilon-poly L-lysine.

Description

technical field [0001] The invention relates to a Streptomyces albicans producing polylysine and its application. Background technique [0002] ε-poly-L-lysine (ε-PL) is a homopolymer formed by the essential amino acid L-lysine through α-carboxyl and ε-amino groups. It is a polypeptide with antibacterial effect and has a broad antibacterial spectrum. It has obvious antibacterial effect on yeast, gram-positive bacteria, gram-negative bacteria and mold. ε-PL can be decomposed into lysine in the human body, which is one of the eight essential amino acids for the human body. All countries in the world allow lysine to be fortified in food. Therefore, ε-PL is a nutritional antibacterial agent. As a natural food preservative in the field of food additives, ε-PL is being paid more and more attention by people, and its safety is obviously higher than other chemical preservatives. [0003] It is known in the prior art that Streptomyces albicans can be used to produce ε-PL, but there...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/02C12R1/47
Inventor 罗施中刘欣高红柳陈慧
Owner BEIJING UNIV OF CHEM TECH
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