Biosensor for detecting silver as well as preparation method and application thereof

A biosensor and reaction technology, applied in the field of biosensors, can solve problems affecting human coordination and vision, complex sample processing, affecting human health, etc., and achieve the effects of superior electron transfer capability, low process cost, and superior electrical conductivity.

Inactive Publication Date: 2014-11-26
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] As an indispensable heavy metal in the production industry, Ag has been widely used in photography, battery and semiconductor industries. In addition, a small amount of Ag has a good bactericidal effect and is often used for sterilization. However, if a large amount of Ag is ingested , will lead to irreversible changes in the color of the human skin, and in severe cases, it will also cause thrombocytopenia, bronchial diseases, affect the coordination and vision of the human body, etc.
At present, it has been pointed out in the literature that silver ions (Ag + ) can inactivate sulfhydryl-containing enzymes and combine with amino groups, imidazole groups and carboxyl groups on various metabolites. More and more information points out that Ag+ has potential toxicity, and it can interact with many potential nutrients, especially Selenium (Se), copper (Cu), vitamin E and vitamin B12 interact to affect human health
[0004] In recent years, on Ag + There are many detection methods, such as electrothermal atomic absorption spectrophotometry (ETAAS), inductively coupled plasma atomic emission spectrometry (ICP-AES), inductively coupled plasma mass spectrometry (ICP-MS), voltammetry, potentiometry and Fluorescence methods, etc., although these methods have high sensitivity and selectivity, the sample processing of these methods is complicated, the instrument is expensive or time-consuming, requires specialized operating technicians, the detection cost is high, and the economic benefit is relatively low

Method used

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  • Biosensor for detecting silver as well as preparation method and application thereof
  • Biosensor for detecting silver as well as preparation method and application thereof
  • Biosensor for detecting silver as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] refer to figure 1 , a biosensor for detecting silver, including a glassy carbon electrode used as a working electrode in a three-electrode system, the surface of the glassy carbon electrode is modified with gold nanoclusters (AuNCs), and the surface of AuNCs is modified with ordered Mesoporous carbon (OMC), OMC and AuNCs form an AuNCs / OMC composite film on the surface of the glassy carbon electrode, and then deposit a layer of gold nanoparticles (AuNPS) on the surface of the AuNCs / OMC composite film to form an AuNCs / OMC / AuNPS composite film , the C1 probe, which can form double strands through C-Ag-C mismatch, was immobilized on AuNCs / OMC by forming gold-sulfur covalent bonds (Au-S) with gold nanoparticles (AuNPS) on the AuNCs / OMC / AuNPS composite film. / AuNPS composite membrane modified biosensor surface; the 3' end "AGT-CTG-ATCG" of the C2 probe is connected to the 5' end "TCA-GAC-TAGC" of the C1 probe through base pairing, and the C3 probe The needle connects the "CC...

Embodiment 2

[0059] A kind of preparation method of the aforementioned biosensor for detecting silver, comprising the following steps:

[0060] S1. Modified AuNCs: Make a glassy carbon electrode, and electrodeposit AuNCs on the surface of the reaction end of the aforementioned glassy carbon electrode to obtain an AuNCs-modified glassy carbon electrode; the scanning potential of electrodeposition is -2.0~2.0V, and the scanning rate is 50mV / s, the number of scanning circles is 4 circles. The manufacturing method of the glassy carbon electrode is manufactured according to a conventional method.

[0061] The AuNCs used were prepared by a method comprising the following steps:

[0062] First set the temperature of the constant temperature oil bath to 37°C, place the three-neck round bottom flask in the oil bath and fix it with a test tube clamp, and start the experiment after it is ready. At 37°C, 5 mL of 10 mM HAuCl 4 The solution and 5 mL of bovine serum albumin (BSA) solution with a conc...

Embodiment 3

[0076] An application of the aforementioned biosensor or the biosensor prepared by the aforementioned preparation method in the detection of silver, comprising the following steps:

[0077] (1) The glassy carbon electrode of the biosensor is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode, and the glassy carbon electrode is used as Tris-HClO 4 After cleaning, soak in a mixed solution of ferricyanide (5.0mM), ferrocyanide (5.0mM) and KCl (0.1mM) to establish a three-electrode system, connect the aforementioned three-electrode system to an electrochemical workstation, and test the AC impedance spectrum;

[0078] (2) Then soak the glassy carbon electrodes in concentrations of 1×10 -5 M, 1×10 -6 M, 1×10 -7 M, 1×10 -8 M, 1×10 -9 Ag of M + In the buffer solution for 2 hours, take it out, wash it, dry it, soak it in the mixed solution of ferricyanide (5.0mM), ferrocyani...

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Abstract

The invention provides a biosensor for detecting silver as well as a preparation method and application thereof. The biosensor comprises a glassy carbon electrode which is used as a working electrode in a three-electrode system; the surface of a reaction end of the glassy carbon electrode is modified with a gold nano-cluster; the surface of the metal nano-cluster is modified with ordered mesoporous carbon; the surface of the ordered mesoporous carbon is modified with a nanogold layer; the surface of the nanogold layer is self-assembled with a C1 probe which is capable of forming a double chain in a C-Ag-C mispairing manner. The preparation method comprises the steps of modifying with the gold nano-cluster, modifying with ordered mesoporous carbon, carrying out electro-deposition on nanogold and assembling the C1 probe. The application method comprises the steps of firstly establishing the three-electrode system, putting a glassy carbon electrode reaction end of the biosensor in a solution to be detected for reacting for 2h and measuring the concentration of silver ions in the solution to be detected according to the change of the concentration and resistance difference of silver ions. The biosensor provided by the invention has the advantages that the manufacture is simple, the service life is long, the anti-jamming capability is high, the detection precision and efficiency are high, etc.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting silver and its preparation method and application. Background technique [0002] Water and land are the most precious natural resources, and they are the basis for our human survival, social economy and agricultural production. However, since the 1980s, with the acceleration of my country's urbanization process and the rapid development of industry and agriculture, a large number of environmental pollutants have been discharged into the environment, resulting in a serious decline in the quality of the water and soil environment, thereby affecting the balance of the ecosystem. Among the large amount of pollutants discharged into the environmental system, heavy metals are an important source that cannot be ignored. According to statistics, since 2009, more than 30 serious heavy metal pollution incidents have occurred in my country. In 2011, the State ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/26
Inventor 汤琳谢霞周耀渝曾光明董浩然章毅陈俊王敬敬汤晶
Owner HUNAN UNIV
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