Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method
A technology of surface growth and sandwich structure, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of uneven surface humidity of filter cloth and fiber fabrics, Intolerance to recycling, the culture system has not entered large-scale production and other problems, to achieve the effect of improving the growth rate and quality of microalgae, improving the utilization rate of light energy, and achieving full growth
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Embodiment 1
[0067] combine Figure 4 One example of the culture system of the present invention is shown.
[0068] Figure 4 The surface growth culture system 1000a can include multiple such as image 3 Shown are the culture units 100 , the light source device 8 disposed between the culture units 100 , and the culture solution circulation device 9 a for supplying the culture solution to the culture units 100 .
[0069] Figure 4 A plurality of culture units 100 in the set are arranged parallel to each other and separated by a distance. At least one light source device 8 is arranged between every two culture units 100 . The light source device can be a double-sided light source to irradiate the culture units on both sides. The light source device 8 can also be a single-sided light source as required.
[0070] Figure 4 The surface growth type culture system 1000a of the present invention comprises a culture solution circulation device 9a, and the culture solution circulation device ...
Embodiment 2
[0074] combine image 3 and Figure 5 Another culture system of the present invention is described. Figure 5 are according to the invention including image 3 A schematic diagram of another embodiment 1000b of a surface-grown culture system of a culture unit. and Figure 4 Similar to the illustrated embodiment, the culture system 1000b may also include one or more image 3 The culture unit 100. Specifically, the culture system 1000b may include a culture unit 100, a culture solution recovery device 3, a liquid supply device 4, a culture solution pool 6, a pressure tank 11, a compressed air source 12, a liquid level gauge 13, a shut-off valve 14, and a shut-off valve 15 , pressure reducing valve 16, exhaust valve 17, and pressure gauge 18.
[0075] Figure 5 As shown, the surface growth culture system 1000b includes a culture fluid circulation device 9b, and an air compression method may be used in the culture fluid circulation device 9b. The device used in this air co...
Embodiment 3
[0098] Embodiment 3 cultivation test
[0099] 3.1. Culture medium composition
[0100] Taking the composition of the culture medium for Chlorella culture as an example, see Table 2 below.
[0101] Table 2 The composition of the culture medium for Chlorella culture
[0102] components
Usage amount
NaNO 3
1mL / L
25g / 100mldH 2 o
K 2 HPO 4
1mL / L
7.5g / 100mldH 2 o
MgSO 4 ·7H 2 o
1mL / L
7.5g / 100mldH 2 o
CaCl 2 2H 2 o
1mL / L
2.5g / 100mldH 2 o
K H 2 PO 4
1mL / L
17.5g / 100mldH 2 o
NaCl
1mL / L
2.5g / 100mldH 2 o
Na 2 CO 3
1mL / L
0.2g / 100mldH 2 o
FeCl 3 ·6H 2 o
1mL / L
0.05g / 100mldH 2 o
EDTA-Fe
1mL / L
trace metal solution
1mL / L
Soil Extract
40mL / L
[0103] Table 3 Recipe of trace metal solution
[0104] components
h ...
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