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Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method

A technology of surface growth and sandwich structure, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of uneven surface humidity of filter cloth and fiber fabrics, Intolerance to recycling, the culture system has not entered large-scale production and other problems, to achieve the effect of improving the growth rate and quality of microalgae, improving the utilization rate of light energy, and achieving full growth

Inactive Publication Date: 2015-02-04
STATE DEV & INVESTMENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of PBR is usually expensive, high operating cost, difficult to maintain, difficult to scale up, and the yield is 5-30g / m 2 / d, far lower than the theoretical expected value of 100-200g / m 2 / d, the theoretical calculation target of industrialization cannot be reached, and the low utilization rate of light energy, the most important direct constraint factor for the large-scale cultivation of microalgae to realize industrialization, still needs to be improved
[0004] In recent years, a kind of semi-dry culture system has appeared, and there have been patent reports, all of which use one or more of filter paper, filter cloth, plastic foam, and fiber fabrics (such as canvas) as matrix materials, but such soft materials It has the following disadvantages: 1) Low mechanical strength, requiring complex support, not suitable for enlargement of large size and space height, and can only be made into low and thin structures, but often self-supporting, porous materials with high rigidity are locked. The water property is relatively limited, and the amount of nutrient solution required for the growth of microalgae cannot be well satisfied; 2) the surface humidity of existing filter cloths and fiber fabrics is not uniform, and the growth of microalgae is not firm; and 3) the existing filter cloth Cloth and fiber fabrics are not resistant to recycling, need to be replaced frequently, increase costs, 4) existing filter cloths and fiber fabrics, etc. are easy to drop impurities such as fibers; in addition, 5) when these flexible materials are used as culture plate surface materials, It has certain limitations in terms of biocompatibility and chemical corrosion resistance.
Therefore, there is currently no possibility of such a culture system entering large-scale production.

Method used

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  • Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method
  • Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method
  • Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] combine Figure 4 One example of the culture system of the present invention is shown.

[0068] Figure 4 The surface growth culture system 1000a can include multiple such as image 3 Shown are the culture units 100 , the light source device 8 disposed between the culture units 100 , and the culture solution circulation device 9 a for supplying the culture solution to the culture units 100 .

[0069] Figure 4 A plurality of culture units 100 in the set are arranged parallel to each other and separated by a distance. At least one light source device 8 is arranged between every two culture units 100 . The light source device can be a double-sided light source to irradiate the culture units on both sides. The light source device 8 can also be a single-sided light source as required.

[0070] Figure 4 The surface growth type culture system 1000a of the present invention comprises a culture solution circulation device 9a, and the culture solution circulation device ...

Embodiment 2

[0074] combine image 3 and Figure 5 Another culture system of the present invention is described. Figure 5 are according to the invention including image 3 A schematic diagram of another embodiment 1000b of a surface-grown culture system of a culture unit. and Figure 4 Similar to the illustrated embodiment, the culture system 1000b may also include one or more image 3 The culture unit 100. Specifically, the culture system 1000b may include a culture unit 100, a culture solution recovery device 3, a liquid supply device 4, a culture solution pool 6, a pressure tank 11, a compressed air source 12, a liquid level gauge 13, a shut-off valve 14, and a shut-off valve 15 , pressure reducing valve 16, exhaust valve 17, and pressure gauge 18.

[0075] Figure 5 As shown, the surface growth culture system 1000b includes a culture fluid circulation device 9b, and an air compression method may be used in the culture fluid circulation device 9b. The device used in this air co...

Embodiment 3

[0098] Embodiment 3 cultivation test

[0099] 3.1. Culture medium composition

[0100] Taking the composition of the culture medium for Chlorella culture as an example, see Table 2 below.

[0101] Table 2 The composition of the culture medium for Chlorella culture

[0102] components

Usage amount

mother liquor

NaNO 3

1mL / L

25g / 100mldH 2 o

K 2 HPO 4

1mL / L

7.5g / 100mldH 2 o

MgSO 4 ·7H 2 o

1mL / L

7.5g / 100mldH 2 o

CaCl 2 2H 2 o

1mL / L

2.5g / 100mldH 2 o

K H 2 PO 4

1mL / L

17.5g / 100mldH 2 o

NaCl

1mL / L

2.5g / 100mldH 2 o

Na 2 CO 3

1mL / L

0.2g / 100mldH 2 o

FeCl 3 ·6H 2 o

1mL / L

0.05g / 100mldH 2 o

EDTA-Fe

1mL / L

trace metal solution

1mL / L

Soil Extract

40mL / L

[0103] Table 3 Recipe of trace metal solution

[0104] components

mother liquor

h ...

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PUM

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Abstract

The invention relates to a surface growth type culture plate having a sandwich structure which comprises outer plates and a core part which is located inside the outer plates, wherein the outer plate is made from rigid water adsorption seepy material plate and the core part is made from a water absorption water-locking material such as a molecular sieve, glass sands, a non-woven fabric, high density sponge, cotton cloth, high molecular water-absorbent resin, super absorbent fibers or a polyurethane water absorbent. The core part is arranged between the outer plates on two sides. The invention further relates to a culture unit which comprises the culture plate, a surface growing culture system and a method for culturing aquatic organisms by using the surface growing culture system. The invention aims at solving the problem that a photobioreactor system for microalgae culture is high in manufacturing and maintenance costs, low in space utilization ratio and low in production efficiency. According to the invention, not only is the culture system economic and cheap, but also the utilization efficiency of light energy, carbon sources and nutrients by microalgae is high and the synthesizing and accumulating speed of secondary metabolites is fast, so that the biomass yield and the secondary metabolism yield in unit floor area or the unit reactor volume or area are improved to a great extent.

Description

technical field [0001] The invention relates to a surface growth culture plate with a sandwich structure, a culture unit comprising the culture plate, a surface growth culture system and a method for cultivating aquatic organisms using the surface growth culture system. Background technique [0002] Microalgae refers to aquatic planktonic algae capable of photosynthesis. Some microalgae are rich in protein and can be used as aquatic bait or livestock feed (such as spirulina); more importantly, some microalgae can synthesize a large amount of secondary metabolites, such as oil, carotenoids, polysaccharides, etc. , These substances are often biologically active substances with extremely high economic value, and can be used in functional foods, food additives, pharmaceuticals, bioenergy and other fields. In particular, extracting microalgae oil through large-scale cultivation of microalgae, and then transforming it into biodiesel is considered to be one of the most important w...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/12C12N1/20
CPCC12M21/02C12M23/04C12M23/58C12M29/00C12M29/18C12M31/02
Inventor 胡强郭惠平张嗣亿喻正保
Owner STATE DEV & INVESTMENT
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