Method for synthesizing high-purity 2-keto acid salt

A keto acid salt, high-purity technology, applied in the separation/purification of carboxylic acid compounds, organic chemistry, fermentation, etc., can solve the problems of cumbersome processing steps in chemical methods, large amount of organic solvents used, unfavorable industrial production, etc., to achieve reaction Mild conditions, reduced production costs, and environmentally friendly production processes
CN104372035BActive Publication Date: 2017-10-31HUNAN BAOLISHI BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN BAOLISHI BIOTECH
Publication Date
2017-10-31

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Abstract

The invention discloses a method for synthesizing high-purity 2-ketonate. The method comprises the following steps: carrying out an enzyme catalysis reaction on an aldehyde compound and a glycine raw material in the catalysis of L-threonine aldolase and L-threonine deaminase compound enzyme to generate a 2-ketonic acid product; carrying out separation purification on the 2-ketonic acid product by chromatography, adjusting the pH to a proper value by adopting alkali carbonate or alkali hydrocarbonate, and sequentially carrying out actived carbon decoloration, concentration crystallization and vacuum drying to obtain 2-ketonate. The preparation method has the advantages of simplicity in operation and mild reaction condition, does not cause pollution by adopting an aqueous phase system, and has low production cost; prepared 2-ketonate has high yield and high purity.
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Description

technical field

[0001] The invention relates to a method for synthesizing 2-keto acid by a novel biological method and separating and purifying it to obtain a pharmaceutical grade keto acid salt, which belongs to the field of biosynthesis. Background technique

[0002] 2-keto acid salts are mainly used in biochemical reagents, in vitro diagnostics, and cosmetic additives. In addition, they are also important pharmaceutical intermediates, mainly used in dietary supplements for fitness and weight loss. Kits configured with 2-butyruvate are widely used in the detection of α-hydroxybutyrate dehydrogenase, and are used as diagnostic basis for myocardial infarction, anemia, leukemia, liver cirrhosis and other diseases. Sodium pyruvate is the substrate for the determination of lactate dehydrogenase, and it is mainly used for the determination of glutamate-alanine aminotransferase activity in liver function tests. Sodium 2-pentanoate is mainly used for hypocalcemia, urticaria, angi...

Claims

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