Supercharge Your Innovation With Domain-Expert AI Agents!

A method for preparing rice bran active peptide by solid-state fermentation of mixed strains

A technology of solid-state fermentation and mixed bacterial species, applied in fermentation, peptide and other directions, can solve the problems of restricting the development and application of functional peptides from rice bran, difficult to scale production, etc. Effect

Inactive Publication Date: 2019-09-10
SHENYANG AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above problems restrict the development and application of rice bran functional peptides, making it difficult to produce them on a large scale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.910 slant strains were respectively transferred to the liquid activation medium, shaken at 28°C and 140rpm for 50 hours, and a small uniform mycelium ball was formed; Divide the four kinds of mycelial balls into pieces of equal size, and prepare the mycelia of Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.91 in a mass ratio of 1:2:4:3. Mixed strains. Add distilled water to the fresh rice bran at a mass-to-volume ratio of 0.4 g / mL, mix evenly, and put it into a 250 mL Erlenmeyer flask. The sterilization condition is 110°C for 5 hours. The mixed strains were inoculated into sterilized rice bran at a ratio of 10% (W:W), and shaken well; put into a mold incubator and cultivated for 6 days at a relative humidity of 70% and a temperature of 28°C. Place the obtained fermentation broth in a centrifuge at 4000rpm for 40min, collect the supernatant, and separate the supernatant throug...

Embodiment 2

[0028] Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.910 slant strains were respectively transferred to liquid activation medium, and shake cultured at 30°C and 150rpm for 48h to form uniform mycelial balls; Divide the four kinds of mycelial balls into pieces of equal size, and prepare the mycelia of Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.91 in a mass ratio of 1:2:4:3. Mixed strains. Distilled water was added to fresh rice bran (purchased from Shenyang Qianzhonglang Rice Industry Co., Ltd.) at a mass-to-volume ratio of 0.45 g / mL, mixed evenly, and then filled into a 250 mL Erlenmeyer flask. The sterilization condition was 115°C for 4 hours. The mixed strains were inoculated into sterilized rice bran at a ratio of 12% (W:W), and shaken evenly; put into a mold incubator and cultivated for 6 days at a relative humidity of 80% and a temperature of 30°C. Place the obtained fermentation broth in a centrifuge at 5000rpm for 30...

Embodiment 3

[0030] Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.910 slant strains were respectively transferred to the liquid activation medium, shaken and cultured at 32°C and 160rpm for 45h, and a small uniform mycelium ball was formed; Divide the four kinds of mycelial balls into equal-sized bacterial flakes, and prepare the mycelia of Aspergillus niger AS3.410, AS3.350, AS3.365 and Aspergillus oryzae AS3.910 in a mass ratio of 1:2:4:3. Mixed strains. Add distilled water to fresh rice bran at a mass-to-volume ratio of 0.5 g / mL, mix evenly, and put it into a 250 mL Erlenmeyer flask. The sterilization condition is 121°C for 3 hours. The mixed strains were inoculated into sterilized rice bran at a ratio of 15% (W:W), and shaken evenly; put into a mold incubator and cultivated for 5 days at a relative humidity of 90% and a temperature of 32°C. Place the obtained fermentation broth in a centrifuge at 5000rpm for 25 minutes, collect the supernatant, and separate th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing rice bran active peptides by using mixed bacteria solid-state fermentation. First, the slanted strains are activated to generate a seed medium, and then the four kinds of mycelia are mixed and inoculated into the sterilized rice bran for solid-state culture, and the mixed state of the rice bran is obtained after separation and drying. The present invention selects high-yielding cellulase, amylase, pectinase, saccharifying enzyme, lipase, protease and other composite microbial flora, and directly ferments rice bran by solid-state fermentation method, which not only improves the nutritional level of the fermented product, but also reduces certain The bitter peptide group is modified and recombined, so that the prepared rice bran peptide is basically free of bitterness and peculiar smell, the production process is simplified, the production cost can be effectively reduced, and there is no pollution, and it is suitable for large-scale industrial production of rice bran active peptide.

Description

technical field [0001] The invention relates to the field of bioactive protein preparation, in particular to a method for preparing rice bran active peptide by solid-state fermentation of mixed strains. Background technique [0002] The essential amino acids in rice bran protein are complete, the biological potency is high, and the amino acid composition is closer to the recommended mode of FAO / WHO, and its efficacy ratio is 2.0-2.5. Rice bran protein is mainly composed of albumin, globulin, glutenin and prolamin in a mass ratio of 37:36:22:5, of which soluble protein accounts for about 70%, and its protein content, application function (such as emulsification, Solubility, stability, etc.) are similar to soybean protein isolate. In addition, the biggest advantage of rice bran protein is hypoallergenic, which is the protein with the lowest allergy among known grains. Therefore, the use of enzymatic hydrolysis of rice bran protein to produce rice bran active peptides with hi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C07K7/06C07K5/00
Inventor 樊金娟阮燕晔朱延姝贾宝艳崔震海张立军朱峰千智伟
Owner SHENYANG AGRI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com