Microbial flocculant proteus vulgaris and application thereof in cane juice clarification

A microbial flocculant, Proteus vulgaris technology, applied in the field of microbial flocculant Proteus vulgaris N-25 and its application in sugarcane juice clarification, can solve the problem of difficult degradation of polyacrylamide, and achieve a short separation and purification cycle , Wide range of sources, fast growth and metabolism

Active Publication Date: 2015-04-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to screen the microbial flocculant producing bacteria Proteus vulgaris N -25, provide a safe, non-toxic, non-polluting microbial flocculant to replace the used organic polymer flocculant polyacrylamide

Method used

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  • Microbial flocculant proteus vulgaris and application thereof in cane juice clarification
  • Microbial flocculant proteus vulgaris and application thereof in cane juice clarification
  • Microbial flocculant proteus vulgaris and application thereof in cane juice clarification

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Take 1mL of activated sludge sample from a sewage treatment plant in Guangzhou and dilute to 10 -1 ~10 -7 After different volume concentration gradients, take 10 -4 、10 -5 、10 -6 、10 -7 Sludge microorganisms were inoculated on agar medium by the spread plate method. After inoculation, it was cultured in a constant temperature incubator at 30°C for 2 days, and various microbial colonies were isolated on the plate medium. Colonies were picked by streaking on plates and placed on petri dishes for expansion. Repeat the above steps to obtain microorganisms with different colony shapes after 5 times of separation and purification, inoculate them on the slant medium, and store them in a refrigerator at 4°C. Then the purified strains were put into 50ml screening medium respectively, placed in shake flasks at 35°C and 150r / min and cultured for 3 days, and the flocculation activity of the obtained culture medium was initially determined by the following method.

[0035] In t...

Embodiment 2

[0042] Inoculate the microbial flocculant Proteus vulgaris N-25 obtained through screening in Example 1 into a test tube containing seed culture solution and incubate at 30° C. for 24 hours. Then it was inoculated into the fermentation broth for fermentation and culture, the fermentation temperature was 35° C., the rotation speed of the shaker was 150 rpm, and the fermentation time was 4 days. The fermentation broth was centrifuged at 6000r / min for 30min, and the supernatant was taken as microbial flocculant.

Embodiment 3

[0044] Take 100ml of sugarcane mixed juice, add 50μL of phosphoric acid with a mass fraction of 85%, adjust the pH to 6.8 with milk of lime, heat and stir to 60°C, and keep warm for 2min. Add sulfite with a mass fraction of 6%, and the amount of sulfur fumigation is 1.8g / L, then use milk of lime to neutralize the pH to 7.1, heat it to 100°C for the second time, and quickly add 2ml of microbial flocculant or 0.1% polyacrylamide 0.2ml, stir well and let stand. Record the time required to settle 50ml. After 30 minutes, measure the volume of the mud bottom, take the supernatant and filter it with a 0.45 μm microporous membrane, measure the light conversion, refraction and absorbance at 560 nm of the filtrate, and calculate the simple purity and color value of the sugarcane juice. The determination and calculation of simple purity and color value are carried out according to the method of "analysis method of sugarcane sugar chemical management", and the results are as follows: fi...

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Abstract

The invention discloses microbial flocculant proteus vulgaris and an application thereof in cane juice clarification. A producing bacterium of a microbial flocculant is proteus vulgaris N-25 which has a collection number of CCTCC (China Center for Type Culture Collection) NO:M2014549 and is collected in the CCTCC on November 5, 2014. The application comprises the following steps: adding phosphoric acid into a cane mixed juice, adjusting the pH value to 6-8 by using lime milk, then heating to 50-70 DEG C, and preserving the heat; and adding sulfurous acid until the sulfur fumigation amount is 1.5-2.0g / L, adjusting the pH value to 7-9, then heating to 60-100 DEG C, adding the microbial flocculant, and standing for 20-40 minutes. The microbial flocculant disclosed by the invention is less in used amount and high in flocculation efficiency, is safe and nontoxic, is easy to degrade biologically, is applied to the sugar industry, and can be used for producing healthier and more environment-friendly products.

Description

technical field [0001] The invention relates to a microbial flocculant, in particular to the microbial flocculant Proteus vulgaris N-25 and its application in sugarcane juice clarification. Background technique [0002] Flocculants are substances that can flocculate and settle solid suspended particles that are not easily settled in liquids. In the process of sugar making, adding a small amount of flocculant to the limed sugarcane juice can make the sediment in the juice flocculate into relatively coarse clumps, thereby increasing the sedimentation speed. At present, polyacrylamide is generally used as a flocculant in sugar factories. Its monomer acrylamide has mutagenic, teratogenic and carcinogenic effects, and it remains in the sugar juice or enters the finished sugar to affect the safety of sugar. Therefore, seeking efficient and safe flocculants has become an urgent problem to be solved in my country's sugar industry. [0003] Microbial flocculants are a kind of metab...

Claims

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Application Information

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IPC IPC(8): C12N1/20C13B20/00C12R1/37
CPCC13B20/002C12N1/205C12R2001/37
Inventor 扶雄罗玉琴侯轶胡松青
Owner SOUTH CHINA UNIV OF TECH
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