Pennisetum purpureum Schum-derived bacillus megaterium and use thereof

A technology of Bacillus megaterium and Elephant Grass is applied in the field of applied microorganisms to achieve good application prospects, enhance resistance, and improve biomass yield.

Active Publication Date: 2015-07-08
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the endophytic bacteria that promote growth in plants mainly come from rice, corn, wheat, sugar cane, apple, citrus, highbush blueberry, mulberry, and apricot, and there are no reports of growth-promoting endophytes from Elephant grass ((1) Kloepper, J.W. 1994. Plant growth promoting bacteria (other systems). In: Okon, J. (Ed.), Azospirillum/Plant Association. CRC Press, Boca Raton, pp. 137–154.; (2) De Silva, A ., Petterson, K., Rothrock, C., Moore, J. Growth promotion of high bush blue berry by fungal and bacterial inoculants. 2000. Hort Science 35, 1228–1230.; (3) Sudhakar, P., Chattopadhyay, G.N., Gangwar, S.K., Ghosh, J.K. Effect of f

Method used

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  • Pennisetum purpureum Schum-derived bacillus megaterium and use thereof
  • Pennisetum purpureum Schum-derived bacillus megaterium and use thereof
  • Pennisetum purpureum Schum-derived bacillus megaterium and use thereof

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Experimental program
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Effect test

Embodiment 1

[0036] Isolation and Culture of Bacillus megaterium pp02 Strain

[0037] In the present embodiment, the growth-promoting plant endophytic Bacillus megaterium pp02 is isolated from the root of Elephant Grass, and Elephant Grass is taken from the Elephant Grass Sumu No. 2 in the experimental field of Jiangsu Academy of Agricultural Sciences, and its separation method is as follows:

[0038] (1) Rinse the root of elephant grass with running water, take 1g as the test material, wash it with 70% alcohol for 5 minutes, rinse with sterile water for 3-5 times, soak in 1% sodium hypochlorite for 20 minutes, Kill microorganisms on the surface of the test material;

[0039] (2) In a sterile environment, the test material was rinsed with sterile water for 3 times, and 100 μL of the last sterile water rinse was applied to the LB solid medium for 48 hours to observe whether there were colonies;

[0040] (3) Add an appropriate amount of quartz sand to the test material for grin...

Embodiment 2

[0045] The cultivation seed production method of bacillus megaterium pp02 is carried out according to the following steps:

[0046] (1) Take the Bacillus megaterium CGMCC 9415 bacterial solution frozen at -70°C and inoculate it on LB solid medium by streaking.

[0047] Cultivate in an incubator at 28°C for 18-20h, pick a single colony and inoculate it in 5mL of LB liquid medium, and culture with shaking at 28°C for 1820h to obtain an activated bacterial liquid;

[0048] (2) Inoculate 1mL of activated bacteria solution into a 500mlL Erlenmeyer flask containing 100mL of LB liquid medium, at 28°C, 150

[0049] Shaking culture at 250r / min for 12h16h to obtain a fermentation broth in logarithmic growth phase with an OD of about 1;

Embodiment 3

[0051] Test of the effect of Bacillus megaterium pp02 on plant growth promotion:

[0052] In order to ensure the homogeneity of the experimental materials, the following experiments of the present invention are all taken as the experimental object with hybrid pennisetum seeds.

[0053] (1) The disinfection process of hybrid pennisetum seeds is as follows: use alcohol with a mass concentration of 70% for 5 minutes, rinse with sterile water for 3-5 times, soak in 10% hydrogen peroxide for 30 minutes, and rinse with sterile water for 5 times;

[0054] (2) Germination of hybrid pennisetum seeds: the sterilized seeds were then placed in 1 / 2 MS medium, 25°C, and cultured in dark for 48 hours to accelerate germination;

[0055] (3) Transplantation of hybrid pennisetum seedlings: Sow the germinated cotton seeds in greenhouse pots filled with 50g of sterilized vermiculite (121°C, 2h), 5 seedlings per pot, 5 pots for each treatment repeat;

[0056] (4) Infection of hyb...

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Abstract

The invention relates to Pennisetum purpureum Schum-derived bacillus megaterium and a use thereof and belongs to the technical field of microbes. The Pennisetum purpureum Schum-derived bacillus megaterium pp02 has a class name of Bacillus megaterium pp02, is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC 9415. The Pennisetum purpureum Schum-derived bacillus megaterium has iron-producing carrier, azotification and IAA production characteristics, has an IAA yield of 10.57 microgrammes per milliliter, has a strong plant root colonization ability and good salt resistance, can grow in a culture medium containing 5% of NaCl (50g/L) and is an excellent plant growth promoting endophyte strain. Through infection of hybrid pennisetum with the Pennisetum purpureum Schum-derived bacillus megaterium, plant salt resistance is substantially improved, plant growth is promoted, and fresh weight, dry weight and height of the plant above the ground are substantially improved. The Pennisetum purpureum Schum-derived bacillus megaterium has important development and application prospects in study of hybrid pennisetum microbial fertilizer and promotion of growth and development of plants in saline marginal land.

Description

technical field [0001] The invention relates to a Bacillus megaterium derived from Elephant grass and its application, belonging to the technical field of applied microorganisms. Background technique [0002] In recent years, with the rise of the biomass energy industry, elephant grass ( Pennisetum purpureum schum.) and hybrid pennisetum have developed into a new type of energy plant due to their large biomass, strong regeneration ability, high content of cellulose and hemicellulose, and strong ecological adaptability. my country has large areas of marginal land suitable for energy such as coastal tidal flats, saline-alkali land, barren land, and barren land. The current biomass production potential exceeds 200 million tons of standard coal. Planting energy grasses such as elephant grass and hybrid pennisetum on marginal land suitable for energy, and developing biomass energy are in line with my country's energy development goal of "not competing for land with food, and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/11
CPCA01N63/00C12N1/20C12N1/205C12R2001/11
Inventor 孙建中李霞耿小燕梅传生蒋建雄高璐傅蕾
Owner JIANGSU UNIV
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