Method for innocently treating drilling mud by using mixed microbial agents
A technology for mixing microorganisms and harmless treatment, which is applied in the field of microbial treatment of drilling mud and waste drilling mud by mixing microorganisms in a harmless manner, which can solve the problem of increasing the amount of mud treatment, increasing the treatment cost, increasing the volume of the mud pool and The number of constructions, etc.
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Embodiment 1
[0094] A method for harmlessly treating drilling mud with a mixed microbial agent, comprising the following steps:
[0095] A. Preparation of mixed microbial agent
[0096] First of all, select Aspergillus niger, Rhizopus niger, P. xylophilus, Bacillus megaterium, Arthrobacter globosa and Halomononas bacteria; the bacteria Bacillus megaterium, Arthrobacter globosa and Halomononas bacteria were cultured in the first stage of solid, and in the second stage of liquid Cultivation and tertiary fermentation culture: the fungi Aspergillus niger, Rhizopus niger and Phylonotus xylophilus are subjected to primary solid culture, secondary liquid culture, tertiary fermentation culture and spore strain culture.
[0097] Concrete strain preparation method is as follows:
[0098] 1. Bacteria (Bacillus megaterium, Arthrobacter globosa, Halomonas) preparation
[0099] Liquid strain formula: glucose 3.5%, peptone 0.25%, beef extract 0.15%, sodium chloride 0.26%, potassium dihydrogen phosphate...
Embodiment 2
[0126] A method for harmlessly treating drilling mud with a mixed microbial agent, comprising the following steps:
[0127] A. Preparation of mixed microbial agent
[0128] First of all, select Aspergillus niger, Rhizopus niger, P. xylophilus, Bacillus megaterium, Arthrobacter globosa and Halomononas bacteria; the bacteria Bacillus megaterium, Arthrobacter globosa and Halomononas bacteria were cultured in the first stage of solid, and in the second stage of liquid Cultivation and tertiary fermentation culture: the fungi Aspergillus niger, Rhizopus niger and Phylonotus xylophilus are subjected to primary solid culture, secondary liquid culture, tertiary fermentation culture and spore strain culture.
[0129] Concrete strain preparation method is as follows:
[0130] 1. Bacteria (Bacillus megaterium, Arthrobacter globosa, Halomonas) preparation
[0131] Liquid strain formula: glucose 3.5%, peptone 0.25%, beef extract 0.15%, sodium chloride 0.26%, potassium dihydrogen phosphate...
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