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High-substrate-affinity graphene-oxide-loaded nano-platinum mimetic peroxidase and preparation method thereof

A technology of peroxidase and graphene, applied in the field of nanotechnology and bionics, can solve the problems of limited application of natural enzymes, limited sources of natural enzymes, harsh storage conditions, etc., and achieve good peroxidase activity and good reproducibility , the effect of low detection limit

Inactive Publication Date: 2015-08-26
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sources of natural enzymes are limited, the purification is complicated and difficult, the storage conditions are harsh, and the operation process is susceptible to denaturation and inactivation caused by various physical and chemical environments. These defects greatly limit the further application of natural enzymes.

Method used

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  • High-substrate-affinity graphene-oxide-loaded nano-platinum mimetic peroxidase and preparation method thereof
  • High-substrate-affinity graphene-oxide-loaded nano-platinum mimetic peroxidase and preparation method thereof
  • High-substrate-affinity graphene-oxide-loaded nano-platinum mimetic peroxidase and preparation method thereof

Examples

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Effect test

example 1

[0045] Weigh 1.2 g of 325 mesh flake graphite and add it to a mixed solution of 144 mL of concentrated sulfuric acid with a concentration of 18.4 mol / L and 16 mL of phosphoric acid with a concentration of 14.7 mol / L. After stirring well, place in an ice bath at 0 °C. Slowly add 7.2 g of potassium permanganate in small quantities to the above mixed solution for low-temperature intercalation reaction. After keeping the ice bath at 0°C and magnetically stirring for 3 hours, the obtained dark green suspension was transferred to a warm water bath at 35°C for medium-temperature reaction, and the temperature was controlled for 1 hour. The resulting suspension was then transferred to a warm water bath at 50°C for high-temperature deep oxidation, and the temperature was controlled for 12 hours. The obtained purple-black suspension was slowly added to 160 mL ice-water mixture, and stirred vigorously for 1 hour. Then, 4.8 mL of 30wt% hydrogen peroxide solution was added dropwise to the...

example 2

[0047] Add 0.575 mL of chloroplatinic acid aqueous solution with a concentration of 10 mg / mL to 9.225 mL of graphene oxide solution with a concentration of 2 mg / mL, mix vigorously for 15 minutes, then add 0.2 mL of 5 mg / mL Aqueous sodium borohydride solution was magnetically stirred for 4 hours. The nano-platinum aqueous solution obtained under the condition of no graphene oxide is brown, while the aqueous solution of graphene oxide-nano-platinum composite is dark brown (see figure 1 A and B in ). After standing for 120 hours, the nano-platinum solution completely settled, while the graphene oxide-nano-platinum composite aqueous solution still showed good dispersion (see figure 1 C and D in ). Scanning the ultraviolet-visible absorption spectra of nano-platinum and graphene oxide-nano-platinum, the graphene oxide-nano-platinum aqueous solution has a maximum absorption value at 230 nm, while the nano-platinum aqueous solution obtained without graphene oxide does not show an...

example 3

[0049] Add 0.575 mL of chloroplatinic acid aqueous solution with a concentration of 10 mg / mL to 9.225 mL of graphene oxide solution with a concentration of 2 mg / mL, mix vigorously for 15 minutes, then add 0.2 mL of 5 mg / mL Aqueous sodium borohydride solution was magnetically stirred for 4 hours. After the resulting solution was freeze-dried, the resulting powder was characterized by Raman spectroscopy, at 1336 cm -1 and 1593 cm -1 The D peak and G peak of graphene oxide appear at the place (see image 3 ).

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Abstract

The invention discloses a high-substrate-affinity graphene-oxide-loaded nano-platinum mimetic peroxidase and a preparation method thereof. The graphene oxide is adopted as a stabilizer for controlling the generation of nano-platinum particles. The invention provides a novel composite nano-material preparation method. The preparation is simple and environment-friendly, high temperature is not needed, and surfactant is not needed. An average particle size of the obtained platinum nano-particles is 9.0nm. The preparation method has good reproducibility, and the obtained product can maintain stability for more than 4 months. The synthesized graphene oxide-nano-platinum composite material has good mimetic peroxidase activity, and can catalyzing 3,3',5,5'-tetramethylbenzidine hydrochloride oxidative coloration with hydrogen peroxide. The material has high affinity to the substrates 3,3',5,5'-tetramethylbenzidine hydrochloride and hydrogen peroxide. The material can be used for detecting low-concentration hydrogen peroxide. A catalysis product light absorbance is linearly related with hydrogen peroxide concentration within a range of 0.1-25mmol / L, and a detection limit is 0.03561mmol / L.

Description

technical field [0001] The invention relates to a method for preparing a platinum nanoparticle composite material by using graphene oxide as a carrier. The composite material has the characteristic of simulating peroxidase with high substrate affinity, and belongs to the field of nanotechnology and bionic technology. Background technique [0002] Nanomaterials have superior properties such as surface effects, quantum size effects, volume effects, and macroscopic quantum tunneling effects, and exhibit unique physical and chemical properties in terms of catalysis, magnetism, light absorption, and thermal resistance. The precious metal platinum is one of the most stable elements in chemical properties, and it is chemically inert on the bulk scale, but the nanoscale platinum particles have special physical and chemical properties, making them suitable for biosensors, photochemical and electrochemical catalysis, and semiconductor fluorescence detection. Needles, optoelectronic de...

Claims

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Application Information

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IPC IPC(8): B01J23/42G01N21/78
Inventor 陈伟林小青邓豪华彭花萍刘爱林
Owner FUJIAN MEDICAL UNIV
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