Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel

Abstract

The invention discloses a synchronous light-electricity sensing method for a dark-field imaging-based solid nano channel. The synchronous light-electricity sensing method comprises the following steps: (1) preparing a semiconductor substrate film coated with a metal nano coating; (2) preparing a metal coated nano channel chip; (3) preparing a light-electricity detection micro pool; (4) imaging by utilizing a metal coated nano channel dark field; (5) recording ion current intensity by utilizing the metal coated nano channel; (6) adding a to-be-detected object for synchronous light-electricity detection; and (7) analyzing multi-channel data. According to the method, the process that a single to-be-detected nano object can pass through the nano channel can be monitored dynamically in real time without introducing dye groups and labeling so as to acquire dynamic information such as the size, the electrification situation, the light property, the chemical structure of the to-be-detected object; the situation of variation of each type of nature of the single to-be-detected object in the moving process can be observed from multiple dimensionalities; multi-channel signals on a large scale can be acquired synchronously; the detection velocity of a nano channel technique is increased; the application field is widened.

Description

technical field [0001] The invention relates to the fields of electrochemical analysis technology and nanometer spectrum technology, in particular to a method for realizing synchronous sensing of nanochannel photoelectric signals by using plasmon resonance scattering combined with ion current detection technology. Background technique [0002] Nanopore Electrochemical Detection Technology (Nanopore Technique) is a single-molecule analysis technology that uses biomolecular self-assembly or semiconductor microfabrication to obtain nanopores with a pore size ranging from 1 nanometer to hundreds of nanometers, and then uses an electric field to drive a single molecule , nanoparticles, polymers and other analytes pass through nanometer-sized channels, which will cause weak signal changes in nanochannels, and study the composition and motion of analytes at the single-molecule and single-particle level to achieve real-time, real-time analysis of single molecules. High-throughput, l...

AI Technical Summary

Problems solved by technology

At present, the technology for photoelectric synchronous reading of nanochannels requires the introduction of dye groups, which will not only affect the movement behavior of the analyte at the level of a single molecule and a single particle, but also the labeling process is complicated. The failure of bleaching and labeling will affect the analysis results of single molecules

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