Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel

A nano-channel and dark-field imaging technology, applied in the field of electrochemical analysis technology and nano-spectroscopy, can solve the problems of influence of motion behavior, complicated labeling process, influence of single-molecule analysis results, etc., to achieve the effect of accelerating the detection rate and expanding the application field.

Active Publication Date: 2015-09-02
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the technology for photoelectric synchronous reading of nanochannels requires the introduction of dye groups, which will not only affect the movement behavior of the analyte at t

Method used

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  • Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel
  • Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel
  • Synchronous light-electricity sensing method for dark-field imaging-based solid nano channel

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0049] Example 1

[0050] A method for optoelectronic synchronous sensing of solid-state nanochannels based on dark-field imaging, including the following steps (see figure 1 ):

[0051] (1) Preparation of semiconductor substrate films coated with metal nanocoatings

[0052] ① A layer of 100nm-thick silicon nitride film 3 is deposited on the single-crystal silicon wafer by chemical vapor deposition technology of micromachining technology, and then lithography is used to etch a square with a side length of 200 microns and a spacing of 5mm on one side of the single-crystal silicon circle. Window 4 to expose the silicon nitride film 3 ; a silicon circle is cut to obtain a substrate 5 including a single square window 4 .

[0053] The microfabrication techniques include chemical vapor deposition, photolithography, chemical etching, electron beam exposure, reactive ion beam etching, magnetron sputtering, electron beam evaporation, atomic layer deposition, molecular beam epitaxy, a...

Example Embodiment

[0096] Example 2

[0097] A method for photoelectric synchronous sensing of solid-state nanochannels based on dark field imaging, comprising the following specific steps:

[0098] (1) Prepare a semiconductor substrate film coated with a metal nanocoating (same as Example 1).

[0099] (2) Preparation of metal-coated nanochannel chips (same as Example 1).

[0100] (3) Preparation of photoelectric detection microcell (same as Example 1).

[0101] (4) Dark-field imaging using metal-coated nanochannels

[0102] Dark-field imaging is performed on the metal-coated nanochannel chip 9 and the change of its spectrum within a certain period of time is continuously monitored. The specific contents are:

[0103] ①Place the photoelectric detection microcell on the microscope stage, perform transmission dark field illumination through the dark field lens, use a 100 W halogen lamp as the white light source, and use the microscope imaging device to image on the first color CCD camera.

...

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Abstract

The invention discloses a synchronous light-electricity sensing method for a dark-field imaging-based solid nano channel. The synchronous light-electricity sensing method comprises the following steps: (1) preparing a semiconductor substrate film coated with a metal nano coating; (2) preparing a metal coated nano channel chip; (3) preparing a light-electricity detection micro pool; (4) imaging by utilizing a metal coated nano channel dark field; (5) recording ion current intensity by utilizing the metal coated nano channel; (6) adding a to-be-detected object for synchronous light-electricity detection; and (7) analyzing multi-channel data. According to the method, the process that a single to-be-detected nano object can pass through the nano channel can be monitored dynamically in real time without introducing dye groups and labeling so as to acquire dynamic information such as the size, the electrification situation, the light property, the chemical structure of the to-be-detected object; the situation of variation of each type of nature of the single to-be-detected object in the moving process can be observed from multiple dimensionalities; multi-channel signals on a large scale can be acquired synchronously; the detection velocity of a nano channel technique is increased; the application field is widened.

Description

technical field [0001] The invention relates to the fields of electrochemical analysis technology and nanometer spectrum technology, in particular to a method for realizing synchronous sensing of nanochannel photoelectric signals by using plasmon resonance scattering combined with ion current detection technology. Background technique [0002] Nanopore Electrochemical Detection Technology (Nanopore Technique) is a single-molecule analysis technology that uses biomolecular self-assembly or semiconductor microfabrication to obtain nanopores with a pore size ranging from 1 nanometer to hundreds of nanometers, and then uses an electric field to drive a single molecule , nanoparticles, polymers and other analytes pass through nanometer-sized channels, which will cause weak signal changes in nanochannels, and study the composition and motion of analytes at the single-molecule and single-particle level to achieve real-time, real-time analysis of single molecules. High-throughput, l...

Claims

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Application Information

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IPC IPC(8): G01N21/69
Inventor 龙亿涛师鑫高瑞应佚伦静超李好问
Owner EAST CHINA UNIV OF SCI & TECH
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