Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting hemachrome from blood flour

A technology for heme and blood meal, applied in the field of heme extraction, can solve the problems of large amount of organic solvent, difficulty in recovery, increase in production cost, etc., and achieve the effects of mild reaction conditions, avoiding environmental pollution and saving production cost.

Inactive Publication Date: 2015-09-23
广东驱动力生物科技集团股份有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The extraction temperature of glacial acetic acid method is high, the solvent recovery is difficult, which increases the production cost, and the improper treatment of waste liquid will cause environmental pollution
The acidic acetone method uses a large amount of organic solvent, which is difficult to recover and has the problem of inconvenient follow-up treatment
Most of the existing technologies use fresh anticoagulated pig blood or blood powder obtained by spray drying anticoagulated pig blood as the raw material for heme extraction, which obviously limits the links of raw material collection, transportation and storage.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting hemachrome from blood flour
  • Method for extracting hemachrome from blood flour
  • Method for extracting hemachrome from blood flour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting hemoglobin from blood meal, the steps are as follows:

[0025] 1) Preparation of Bacillus subtilis: In a 250mL Erlenmeyer flask, put 100mL of beef extract peptone liquid medium, inoculate a ring of activated Bacillus subtilis strains, culture at 30°C for 48 hours and set aside for later use. Post-log or stationary phase at a density of 1.0×10 9 ~5.0×10 10 CFU / mL;

[0026] 2) Weigh 10g of blood meal, add 200mL of distilled water, and homogenize for 0.5h to obtain blood meal solution;

[0027] 3) Use 2mol / L sodium hydroxide solution to adjust the pH of the blood meal solution to 9.0, add 4% of the Bacillus subtilis, and ferment at 30°C for 24 hours at a speed of 70rpm to prepare a fermentation broth;

[0028] 4) Centrifuge the fermentation broth obtained in step 2) at 3000r / min for 15min, collect the upper layer solution, then adjust the pH of the solution to 4.0 with 1mol / L hydrochloric acid, centrifuge at 3000r / min for 15min, wash the obtaine...

Embodiment 2

[0032] A method for extracting hemoglobin from blood meal, the steps are as follows:

[0033] 1) Preparation of Bacillus subtilis: In a 250mL Erlenmeyer flask, put 100mL of beef extract peptone liquid medium, inoculate a ring of activated Bacillus subtilis strains, culture at 30°C for 48 hours and set aside for later use. Post-log or stationary phase at a density of 1.0×10 9 ~5.0×10 10 CFU / mL;

[0034] 2) Weigh 30g blood meal, add 500mL distilled water, homogenize for 1 hour, and obtain blood meal solution;

[0035] 3) Adjust the pH of the blood meal solution to 8.0 with 2mol / L sodium hydroxide solution, add 6% Bacillus subtilis, ferment at 40°C, shake at a speed of 120rmp for 12h, and prepare the fermentation broth;

[0036] 3) Centrifuge the fermentation broth in step 2) at 3000r / min for 20min, collect the upper layer solution, then adjust the pH of the solution to 5.0 with 1mol / L hydrochloric acid, centrifuge at 3000r / min for 20min, wash the obtained precipitate with di...

Embodiment 3

[0039] A method for extracting hemoglobin from blood meal, the steps are as follows:

[0040] 1) Preparation of Bacillus subtilis: In a 250mL Erlenmeyer flask, put 100mL of beef extract peptone liquid medium, inoculate a ring of activated Bacillus subtilis strains, culture at 30°C for 48 hours and set aside for later use. Post-log or stationary phase at a density of 1.0×10 9 ~5.0×10 10 CFU / mL;

[0041] 2) Weigh 10g of blood meal, add 300mL of distilled water, homogenize for 0.5h to obtain blood meal solution;

[0042] 3) Use 1mol / L hydrochloric acid solution to adjust the pH value of the blood meal solution to 6.0, add 3% of Bacillus subtilis, and ferment at 35°C (100rpm) for 48 hours to prepare the fermentation broth;

[0043] 4) Centrifuge at 3500r / min for 10min, collect the upper layer solution, then adjust the pH of the solution to 4.5 with 1mol / L hydrochloric acid, centrifuge at 3500r / min for 10min, wash the obtained precipitate with distilled water and absolute ethan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting hemachrome from blood flour. The method for extracting the hemachrome from the blood flour comprises the following steps of (1), preparing bacillus subtilis; (2), preparing the aqueous solution of the blood flour; (3), inoculating the bacillus subtilis in the aqueous solution of the blood flour, and preparing fermentation broth; (4), obtaining hemachrome. By using the method which is provided by the invention, the extraction rate of the hemachrome is improved; the hemachrome is extracted from the blood flour by utilizing an environment-friendly and pollution-free method; reaction conditions are mild; a product has no toxic and side effects; not only are problems that an environment is polluted, a reagent is high in toxicity and difficult to recover, and the like are avoided, but also the production cost is saved at the same time; the safety of the product is improved; the utilization of a blood resource is increased; the inconvenience that a fresh blood is used as a raw material is solved.

Description

technical field [0001] The invention relates to a method for extracting hemoglobin, in particular to a method for extracting hemoglobin from blood meal. Background technique [0002] Heme is a kind of natural porphyrin iron compound with important physiological function and use value, which widely exists in the muscle and blood of various animals. Heme participates in the process of respiration and red blood cell formation and maturation in the body, and has important functions such as removing hydrogen peroxide in the body, promoting the production of antibodies in the body, and catalyzing the conversion of β-carotene into vitamin A. Heme is rich in iron, which is easily absorbed and utilized by the body. The absorption rate is about five times that of non-heme iron. Therefore, heme has a good anti-anemia effect and can also be used as an iron supplement or nutritional enhancer . In the food industry, heme can replace synthetic pigments to ensure food safety. In addition...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/18C12R1/125
Inventor 刘平祥郑艳艳
Owner 广东驱动力生物科技集团股份有限公司