a kind of no 3 ‑ Ion Detection Reagents and Applications
An ion detection and detection reagent technology, applied in the field of NO3- ion detection reagents, can solve the problems of large ionic radius, not many fluorescent probes, etc., and achieve good membrane penetration, high sensitivity fluorescence enhancement, good water solubility Effect
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Embodiment 1
[0033] Weigh 390 mg of 9,10-bispyridylvinyl anthracene, put it into a 25 mL round bottom flask, add 8 mL of anhydrous N,N-dimethylformamide, add 400 mg of bromooctane, and heat to reflux for 24h . After cooling, it was filtered, and the solid was rinsed with dichloromethane and petroleum ether, and the recrystallized red solid powder a (230 mg, yield 30%) was dissolved in acetonitrile solution.
Embodiment 2
[0035] (1) Preparation of probe a solution: Weigh 7.7 mg of a, dissolve it in double distilled water, and prepare a 10.0 mL solution with a concentration of 1.00×10 -3 mol L -1 ; Dilute stepwise with distilled water to 1.00×10 as needed -5 mol L -1 concentration; (2) NO 3 - Standard solution: Weigh 30.4 mg of tetrabutylammonium nitrate, dissolve it in twice distilled water, and prepare a 100mL solution, NO 3 - The concentration is 2.00×10 -3 mol L -1 ; Dilute step by step with twice-distilled water to an appropriate concentration as required; the preparation of other coexisting metal ion solutions is the same.
Embodiment 3
[0037] Add the aqueous stock solution of probe a (1.00×10 -4 mol L -1 , 1mL), anion NO 3 - (1.00×10 -3 mol L -1 , 1 mL). Dilute to the mark with double-distilled aqueous solution, shake well, and transfer to a 3cm quartz cuvette for fluorescence spectrum measurement. The excitation wavelength of probe a solution fluorescence spectrometry was 470 nm. In aqueous solution, probe a (1.00×10 -5 mol L -1 ) itself has very weak fluorescence emission, and basically no fluorescence can be observed under ultraviolet light. Join NO 3 - (1.00×10 -4 mol L -1 ) significantly enhanced the fluorescence of the probe a solution at 575 nm, and in other experiments the anion F - , Cl - , Br - , HSO 4 - , SO 4 2- , AcO - , BF 4 - , H 2 PO 4 -There was no obvious signal response to the probe a solution, indicating that the probe a was only responsive to NO 3 - Selective fluorescence enhanced detection response performance (attached image 3 with Figure 4 ).
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