Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mold for manufacturing aneurysm model and method

An aneurysm and mold technology, applied in the field of medical animal model establishment, can solve the problems of blood vessel damage, complicated operation, and many human interventions.

Inactive Publication Date: 2015-11-04
高不郎
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the pathogenesis of aneurysms is not very clear. The aneurysm animal models established by existing methods, such as the aneurysm animal model established by transgenic and gene knockout technology, the aneurysm animal model established by elastase perfusion arterial method, calcium chloride Aneurysm animal models established by arterial infiltration, aneurysm animal models established by allograft or autologous vascular transplantation, etc. These models have the disadvantages of high cost, complicated operation, excessive human intervention, and too much emphasis on damage to blood vessels caused by a single factor. Does not truly reflect the pathogenesis of human aneurysms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mold for manufacturing aneurysm model and method
  • Mold for manufacturing aneurysm model and method
  • Mold for manufacturing aneurysm model and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Such as figure 1 As shown, the mold used in this embodiment is an open circular ring, and the material of the mold is polytetrafluoroethylene; polytetrafluoroethylene is a polymer compound formed by polymerization of tetrafluoroethylene, which has excellent chemical stability and corrosion resistance. Sex, sealing, high lubricity and non-stickiness, electrical insulation and good anti-aging endurance, it is physiologically inert, non-toxic, and has no adverse reactions when implanted as artificial blood vessels and organs for a long time. The mold height H, that is, the thickness of the ring is 1 cm, the middle diameter, that is, the width of the widest part L of the mold, that is, the outer diameter of the ring is 3 cm, and the height of the two ends is slightly lower than the middle, and the height of the two ends is 0.8 cm. The opening width l is 0.3 cm.

[0033] The establishment method of the aneurysm animal model of the present embodiment, its specific embodiment...

Embodiment 2

[0038] In this embodiment, the selected mold is concave, such as figure 2 As shown, the material of the mold is biological titanium alloy, which has strong corrosion resistance, good compatibility with human cell tissue, no allergic reaction, high strength and low elastic modulus; the height H of the mold is 1.1cm , the distance between the middle opening of the mold, that is, the width l of the mold opening, is 0.45cm, and the distance between the two ends, that is, the length of the widest part L of the mold, is 0.9cm.

[0039] The establishment method of the aneurysm animal model of the present embodiment, its specific embodiment comprises the following steps:

[0040] Step 1. Randomly select 40 healthy clean rats, male or female, weighing 395-405 g, and randomly divide them into two groups, 20 rats in the control group and 20 rats in the experimental group. After fasting for 12 hours, the rats in the experimental group were anesthetized. The anesthetic was 2% pentobarbit...

experiment example 1

[0047] Experimental example 1: Pathological section and HE (hematoxylin-eosin staining) staining of vessel wall

[0048] In the 4th, 6th, and 8th months after the operation, select 6 rats of the control group in Example 1, and randomly select 6 rats in the experimental group to complete the modeling in Example 1, and perform the following operations on the rats: use Rats were anesthetized with 2% pentobarbital at a dosage of 2mL / Kg, the skin was prepared, disinfected with iodine, the skin and muscles were gradually incised using surgical methods, the abdominal cavity of the rat was opened, the abdominal aorta was separated, and the mold pad was removed The curved section of the high abdominal aorta; the curved section of the abdominal aorta was washed with normal saline, and then immediately fixed in neutral formalin fixative for 5-10 hours to form an abdominal aorta specimen. Embed the above abdominal aorta specimen, the steps are as follows:

[0049] 1) Dehydration: The volum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The present invention discloses a mold for manufacturing an aneurysm model and a method for using the mold to establish an animal aneurysm model. The mold is in the shape of a ring, a polygon, an oval or a letter n, a letter Omega, a letter U and a gap with an opening. The opening width of the mold is 0.3 to 1 cm, the height of the mold is 1 to 1.5 cm, and the opening width of the mold is 10% to 90% of the length of the widest part of the mold. A modeling method is that the artery of an animal is exposed, the artery is separated, the mold is placed at the lower part or one side of the artery such that a part of artery close to the mold is bent to the other side, finally MMP-2 and MMP-9 expression amounts in the animal increase or the wall thickness of a blood vessel opposite to the part of the animal blood vessel close to the mold is thinned, and a model is formed. According to the mold and the method, the animal aneurysm model can be simply and repeatedly made, the pathogenesis process of a human body aneurysm is realistically simulated, and the research of the aneurysm pathogenesis and treatment drugs is facilitated.

Description

technical field [0001] The invention relates to a mold and a method for manufacturing an aneurysm model, belonging to the field of medical animal model establishment. Background technique [0002] Aneurysm is a tumor-like protrusion formed due to the local weakness of the arterial wall, which will cause serious consequences after rupture and bleeding, even life-threatening. Aneurysms can form at any location. According to the location of the aneurysm, it can be divided into peripheral aneurysm, abdominal aortic aneurysm, thoracoabdominal aortic aneurysm, aortic dissection aneurysm, and visceral aneurysm. Severe pain when aneurysm compresses peripheral nerves or ruptures, thrombus or plaque in the tumor cavity causes distal arterial embolism, resulting in ischemia or necrosis of limbs, organs, etc. For example, rupture of cerebral aneurysm can cause cerebral hemorrhage, resulting in death and disability The rate is very high; aortic aneurysm rupture and massive hemorrhage ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D1/00
Inventor 高不郎刘国超张学敬范琼瑛
Owner 高不郎
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com