Method for extracting multiple active ingredients of blueberry fruits

An active ingredient and blueberry technology, which is applied in the field of agricultural product processing and comprehensive utilization of resources, can solve the problems of underutilization of biologically active ingredients, and achieve the effects of improving resource utilization, good quality, and increasing product added value.

Inactive Publication Date: 2015-11-11
张家界立功旅游农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other biologically active components are not fully utilized after extracting anthocyanins from blueberries

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Rabbit-eye blueberry 10kg, variety Yuanlan, produced in Ligongqiao, Yongding District, Zhangjiajie City, Hunan Province. Clean the fresh fruit, extract the juice with a screw juicer, squeeze and separate the pulp and skin seeds, and obtain 7.62kg of fresh fruit juice, which is used for processing beverages or brewing wine; the peel dregs are dried with hot air circulation, crushed, and passed through a 20-mesh sieve to obtain dried peel Slag powder 486g, set aside.

[0025] Add 5kg of purified water to the cleaned microwave extraction tank, weigh 400g of dried peel powder, 2g of pectinase, 4g of cellulase, and 2g of sodium hydroxide, stir, heat to the temperature of the feed liquid at 32°C, and enzymatically hydrolyze for 6 hours, Microwave extraction for 60 minutes, control the extraction temperature at 40°C, microwave load power 500W / L, after the extraction is complete, let it sit for 1 hour, put the supernatant into a covered blue plastic bucket for temporary storage...

Embodiment 2

[0030] Pick 10kg of fresh South Highbush blueberries, the variety Misti, from Ligongqiao, Yongding District, Zhangjiajie City, Hunan Province. After cleaning the fresh fruit, use a screw extractor to separate the pulp and seeds to obtain 7.84 kg of fresh fruit juice, which is used for wine making; the peel dregs are dried by hot air circulation, crushed, and passed through a 20-mesh sieve to obtain 434 g of dried peel dregs powder for later use.

[0031] Add 6kg of purified water to the cleaned microwave extraction tank, weigh 400g of dried peel residue powder, 10g of pectinase, 30g of cellulase, and 2g of sodium hydroxide, stir, heat to the temperature of the material liquid at 32°C, and enzymatically hydrolyze for 5 hours. Microwave extraction for 40 minutes, control the extraction temperature at 40°C, and the microwave load power at 500W / L. After the extraction is complete, let it stand for precipitation for 1 hour. Put the supernatant into a covered blue plastic bucket for te...

Embodiment 3

[0036] Picking 10kg of fresh North Highbush blueberries, variety Elliott, produced in Ligongqiao, Yongding District, Zhangjiajie City, Hunan Province. Clean the fresh fruit, extract the juice with a screw juicer, squeeze and separate the pulp and skin seeds, and obtain 7.75 kg of fresh fruit juice, which is used for wine making; the peel dregs are dried by hot air circulation, crushed, and passed through a 20-mesh sieve to obtain 443 g of dried peel dregs powder ,spare.

[0037] Add 4kg of purified water to the cleaned microwave extraction tank, weigh 400g of dried peel residue powder, 10g of pectinase, 20g of cellulase, and 2g of sodium hydroxide, stir, heat to the temperature of the material liquid at 35°C, and enzymatically hydrolyze for 4 hours. Microwave extraction for 50 minutes, control the extraction temperature at 45°C, microwave load power at 500W / L, after the extraction is complete, let it sit for 1 hour, put the supernatant into a covered blue plastic bucket for te...

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PUM

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Abstract

A method for extracting multiple active ingredients of blueberry fruits comprises the steps that dried and smashed blueberry peel residues are firstly subjected to enzyme hydrolysis, then microwave extraction and solid-liquid centrifugal separation are performed, refined filtration is performed by using micropore filter bags, and filtrates are combined; discoloring and impurity removal are conducted on the mixed filtrate by using an ultra-filtration membrane, membrane re-separation is performed, vacuum concentration, alcohol precipitation and drying are conducted on the trapped filtrate to obtain a blueberry polysaccharide extract; passing-column absorption is conducted on the ultra-filtrated dialyzate through macroporous resin, then ethyl alcohol is used for elution and desorption, a desorbed solution is separated and concentrated by using a nano-filtration membrane, and vacuum concentration and freeze drying are conducted on the trapped solution to obtain a blueberry anthocyanin and original anthocyanin combined extract; nano-filtration membrane dialysate is subjected to further separation and dehydration by using a reverse osmosis membrane, vacuum concentration and spray drying are conducted on the trapped solution to obtain a blueberry ellagic acid etract. By means of the method, multiple effective ingredients including polysaccharide, original anthocyanin, anthocyanin, ellagic acid and the like of blueberries can be obtained, the extracts are higher in purity, good in quality, and a production process is green and capable of saving energy and is environmentally friendly.

Description

technical field [0001] The invention relates to the fields of agricultural product processing and resource comprehensive utilization, and specifically provides a method for extracting various high-value-added active ingredients from blueberry fruits. Background technique [0002] Blueberry is a perennial deciduous or evergreen shrub of the genus Vaccinium. It is a small berry fruit tree with extremely high economic value and market prospects. [0003] Blueberry fruit is rich in nutrients and bioactive components. 18 essential amino acids such as fructose and organic acids and nutrients such as A, B, C, E, K vitamins and trace elements; anthocyanins, proanthocyanidins, ellagic acid, folic acid, organic acids, polysaccharides, superoxide It contains various bioactive components such as dismutase, and has various nutritional and health effects on human health. [0004] Blueberry is the crop with the highest water-soluble anthocyanin content in fruits and vegetables, and the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45A61K8/97A23L1/29A23L33/00
Inventor 许玉泉陈国华侯志功王文茂
Owner 张家界立功旅游农业发展有限公司
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