Usage of streptomyces psammoticus and vanillin production method

一种生产方法、沙链霉菌的技术，应用在沙链霉菌的用途及香兰素的生产领域，能够解决反应条件苛刻、有副产物、不易分离等问题，达到操作简单、污染少的效果

Active Publication Date: 2015-12-09
XIAMEN OMIC BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 4) p-Hydroxybenzaldehyde, p-nitrotoluene method, p-cresol method: these three methods have not yet industrialized production due to various reasons (p-Hydroxybenzaldehyde is due to the high price of raw materials; the p-cresol method has harsh reaction conditions and low yield ; The p-nitrotoluene method requires relatively large equipment)
[0009] 5) Safrole method: safrole is mainly derived from camphor oil, the reaction route is long, and there are by-products, which are not easy to separate
However, the separation of enzymes and the directional transformation and modification of enzymes need further research, and this method is still in the research stage.

Method used

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  • Usage of streptomyces psammoticus and vanillin production method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the production of natural vanillin (erlenmeyer flask shaking fermentation)

[0047] Prepare seed culture medium, the composition of which is as follows (g / L): glucose 20, potassium dihydrogen phosphate 3, urea 5, magnesium sulfate 0.3, sodium chloride 1.0, yeast extract powder 5, corn steep liquor powder 5, ammonium sulfate 6 , Calcium carbonate 0.2 The solvent is water, the initial pH is 7.2. Sterilize at 121°C for 20min.

[0048] Preparation of fermentation medium, the composition of the medium is as follows (g / L): glucose 30, potassium dihydrogen phosphate 5, urea 5, magnesium sulfate 0.6, yeast extract powder 8, ammonium sulfate 3, calcium carbonate 1.0 ferulic acid 20, solvent It is water, the initial pH is 7.2. Sterilize at 121°C for 20min.

[0049]Seed preparation: Aseptically transfer the OMK-4 strain in a low-temperature glycerol tube to a fresh, sterile solid plate, activate it at 30°C for 2 days, pick the colony and inoculate it into a 500ml tr...

Embodiment 2

[0051] Embodiment 2: the production of natural vanillin (stirred reactor fermentation)

[0052] The preparation of seed culture medium and fermentation medium is the same as in Example 1.

[0053] Seed preparation: Aseptically transfer the OMK-4 strain in a low-temperature glycerol tube to a fresh, sterile solid plate, activate at 30°C for 2 days, pick colonies and inoculate them in a 3L seed tank with 1.8L seed medium for seed cultivation, ventilate The stirring speed is 300 rpm, and the culture is carried out at 30° C. for 13-18 hours to obtain the seed solution.

[0054] Fermentation of natural vanillin: Put 10.2L of the prepared fermentation medium into a 20L fermenter, sterilize at 121°C for 20min, then put 1.8L of the cultured seed liquid into a 20L fermenter for fermentation, and the fermentation temperature is 37 ℃, stirring speed 400rpm, ventilation ratio 1; 0.2. The fermentation period is 90h. The concentration of natural vanillin in the fermented liquid measured ...

Embodiment 3

[0055] Embodiment 3: the extraction of natural vanillin

[0056] Taking temperature is 80 ℃, and embodiment 2 natural vanillin concentration is 12L of fermented liquid of 15g / L, carries out ceramic membrane filtration, 10L filtrate is carried out ultrafiltration (UF), ultrafiltrate is carried out reverse osmosis (RO), finally obtains 5L of concentrated solution, adjust the pH of the concentrated solution to 5-6, cool to 0-4°C to crystallize for 4 hours, suction filter to obtain crude natural vanillin, and dry to obtain 144g of crude product with a purity of 98%.

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Abstract

The invention discloses a usage of streptomyces psammoticus and a vanillin production method. The streptomyces psammoticus is Streptomyces psammoticus OMK-4 and used for vanillin production. The streptomyces psammoticus is adopted for production of vanillin according to a fermentation method which includes steps of strain activation, seed culture, fermentation, extraction and the like. By means of the microbiological fermentation method for vanillin production, raw materials including ferulic acid and the like are adopted for generation of a target product through microbial metabolism. The vanillin production method is implemented under the conditions of low temperature and low pressure, thereby having the advantages of high safety, operational simplicity, less pollution and environment friendliness.

Description

technical field [0001] The invention relates to the use of Streptomyces sandra and a production method of vanillin. Background technique [0002] Vanillin, also known as vanillin, molecular weight: 152.14. White to light yellow crystals, melting point 81°C, boiling point 284-285°C, relative density 1.060, flash point 147°C, solubility: 10g / L (25°C), soluble in organic solvents such as ethanol. Found in Peru balsam, clove bud oil, vanilla, coffee, grapes, brandy, has vanilla smell, very sweet taste. [0003] Vanillin is not only an important spice, but also an important chemical raw material and an important pharmaceutical intermediate. In food, because of its vanilla bean aroma, it is widely used as a fixative in various foods. In medicine, vanillin is a raw material for the synthesis of pharmaceutical intermediates, and can be used to produce raw materials for the treatment of high blood pressure, heart disease, and commonly used drugs for skin diseases. [0004] 1. Che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/24C12R1/465
CPCC12N1/20C12P7/24C12N1/205C12R2001/465
Inventor 赵希景邢晨光黄志强刘伟龚宏灿
Owner XIAMEN OMIC BIOTECH CO LTD
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