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Lactobacillus plantarum and application thereof in fermenting cured meat products

A technology of Lactobacillus plantarum and cured bacon, which is applied in the field of microorganism and meat food fermentation, can solve the problems of less research and application of starter, acidic taste of fermented sausage, and inconformity with eating habits, etc., to improve the nutritional value of products and promote The degradation of protein and the formation of flavor, and the effect of inhibiting the growth and reproduction of spoilage microorganisms

Active Publication Date: 2016-04-20
CHINA MEAT RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to obtain starter cultures with excellent properties, scholars at home and abroad have done a lot of research in this area, but they mainly focus on fermented sausages. Fermented sausages taste sour and can be eaten directly after fermentation. accepted
At this stage, the production of traditional cured meat products still adopts the natural fermentation method, and there are few cured products that are artificially fermented with the addition of starter cultures, and there are few related researches and applications of starter cultures that are really suitable for traditional cured meat products in my country Therefore, the breeding of strains with excellent traits suitable for traditional cured meat products in my country is a key technical problem to be solved urgently in the production and development of cured meat products

Method used

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  • Lactobacillus plantarum and application thereof in fermenting cured meat products
  • Lactobacillus plantarum and application thereof in fermenting cured meat products
  • Lactobacillus plantarum and application thereof in fermenting cured meat products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The biological characteristic of embodiment 1 plant Lactobacillus (Lactobacillus plantarum) CGMCCNo.11341

[0046] 1. Determination of growth curve

[0047] Lactobacillus plantarum (Lactobacillus plantarum) CGMCCNo.11341 preserved in glycerol tubes was inoculated into MRS nutrient broth for activation twice, and the fresh bacterial solution was transferred to fresh MRS broth medium at 2% inoculum, at 37°C, Shake culture at 200r / min, take samples every 2h for the first 24h, take samples at 30h, 36h and 48h after 24h, measure the number of viable bacteria and the turbidity of the bacterial suspension by pouring the plate method and photoelectric turbidimetry (OD600) respectively, each Time point to do 3 repetitions.

[0048] 2. Determination of acid production curve

[0049] Bacterial strain cultivation and regular sampling were the same as above, and the pH value of the bacterial suspension was measured with a pH meter, with 3 repetitions at each time point.

[0050] ...

Embodiment 2

[0051] Embodiment 2 fermentation agent and preparation method thereof

[0052] The strains stored in glycerol tubes were transferred and activated twice, then inoculated in MRS broth medium at an inoculation ratio of 2%, and cultured at 37°C and 200r / min for 24 hours to obtain a bacterial suspension, which was centrifuged at 5000rpm for 15min and then discarded. Add an equal volume of sterile normal saline to re-suspend the bacteria in the upper culture medium, then centrifuge to discard the supernatant, then add 1 / 10 of the volume of the bacterial suspension in normal saline and mix to obtain a concentrated bacterial solution, add 4 times the volume of the concentrated bacterial solution After lyoprotectant (10% skimmed milk), freeze-drying makes starter, adopts gradient dilution and pour plate method to record that the bacterial activity in this dry powder starter is 10 10 CFU / g.

Embodiment 3

[0053] Application of embodiment 3 leavening agent in traditional bacon products

[0054] 1) Select 10kg of fresh rump meat with a fat-to-lean ratio of 3:7, remove impurities on the meat surface, and divide it into strips about 30cm long, 5cm wide, and 5cm thick.

[0055] 2) Accurately weigh 0.01g starter dry powder, and configure 700g, 2% glucose solution to dissolve it, according to the weight of the raw meat and the volume of the injection solution, evenly inject the glucose solution in which the starter has been dissolved into the raw meat, so that The amount of Lactobacillus plantarum (Lactobacillus plantarum) CGMCCNo.11341 added in raw meat is about 10 4 CFU / g raw meat.

[0056] 3) Vacuum tumbling and pickling, using a vacuum tumbling machine, the tumbling time is 10 hours; each work is 20 minutes, the interval is 40 minutes, and the effective tumbling time is 200 minutes.

[0057] 4) Weigh 0.6g of sodium nitrite, dissolve it in a small amount of water, and apply it ev...

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Abstract

The invention relates to fermentation of microorganism and meat products, and in particular discloses lactobacillus plantarum and application of the lactobacillus plantarum. The lactobacillus plantarum CMRC 10L is characterized in that the preservation number of the lactobacillus plantarum CMRC 10L is CGMCC (China General Microbiological Collection Center) No.11341, and the lactobacillus plantarum CMRC 10L is preserved in the on September 8th, 2005. A lactobacillus plantarum strain is separated from self-made traditional cured meat products of a peasant family, the growth of the lactobacillus plantarum on an MRS culture medium is good, the acid production speed is fast, the lactobacillus plantarum has the biological characteristics of salt resistance, nitrous resistance, cold injury resistance and inhibition on growth and propagation of spoilage microorganisms and is a good strain for preparing a fermenting agent for production. According to the lactobacillus plantarum disclosed by the invention, by adding the lactobacillus plantarum strain as the fermenting agent, the pH (Potential of Hydrogen) value of meat products can be quickly reduced at the early stage of fermentation, and the growth and propagation of spoilage bacteria and pathogenic bacteria and the generation of toxin are inhibited; meanwhile, objectionable odor of a fishy smell, a cowy / goaty smell and the like of meat are improved, the texture of the meat products is improved, and the degradation of protein in the meat and the flavor forming are promoted at the same time, so that the fermenting and ripening time of the products is shortened, and the nutritive value of the products is increased.

Description

technical field [0001] The invention relates to microorganisms and meat food fermentation, in particular to a plant lactobacillus and its application. Background technique [0002] Traditional cured meat products have the characteristics of high salt and low water activity, are easy to store, have unique flavors, and have high nutritional value. Bacon is an outstanding representative of traditional Chinese cured meat products. It is mainly made of livestock and poultry meat, marinated with salt, sugar, spices and other auxiliary materials, and then processed by baking, drying or smoking. products. It has distinctive features such as simple processing technology, unique flavor, convenient storage and transportation. However, there are still problems that cannot be ignored in the processing and production of traditional bacon products, such as unstable product quality, long production cycle, and bacterial contamination. This is mainly due to the fact that traditional bacon ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L13/40C12R1/25
Inventor 潘晓倩王守伟乔晓玲成晓瑜张顺亮赵冰陈文华李家鹏曲超艾婷许典
Owner CHINA MEAT RES CENT
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