Bletilla striata polysaccharide and paeonol composition product and application thereof

A technology of paeonol and composition, which is applied in the field of natural medicine application, can solve problems such as the loss of active ingredients of paeonol, achieve the effects of reducing production costs, increasing functions, and inhibiting the growth of germs

Active Publication Date: 2016-04-27
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The above documents show that although the use of white and neutral polysaccharides can effectively prevent the loss of medicinal active ingredients, measures to prevent the conversion of other substances have not been adopted during the preparation process, and the active ingredients such as paeonol have suffered serious losses.

Method used

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  • Bletilla striata polysaccharide and paeonol composition product and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation method of Baiji polysaccharide

[0047] Crush 100kg of dry white powder, directly add 200L of petroleum ether and stir for 20min, then let it stand for 1 hour to separate and remove the upper layer solution containing petroleum ether to obtain Precipitation I; add 250L of 85% ethanol aqueous solution to Precipitation I, stir and extract for 70min , let it stand for 60 minutes, and removed the upper layer solution containing ethanol by layers to obtain Precipitation Ⅱ. Add 300L of water to the precipitate II, stir and heat to 85°C, keep warm for 30min, centrifuge at 5000rpm for 15min to obtain a supernatant, and remove the precipitate. Concentrate the supernatant under reduced pressure at 50-65°C to 1 / 15 of the original volume, then add 95% ethanol until the final concentration reaches more than 75%, ethanol precipitation for 15 hours, and filter once with a 0.22 μm ceramic membrane to obtain the filter residue. It is white and polysacchari...

Embodiment 2

[0048] Embodiment 2: Preparation of human epidermal growth factor (hEGF) hydrolyzate

[0049] Recombinant human epidermal growth factor (hEGF) is a small peptide consisting of 53 amino acid residues. Recombinant human epidermal growth factor (purchased from Guangzhou Enkepeptide Pharmaceutical Technology Co., Ltd.) is hydrolyzed under the action of papain, 10g recombinant human epidermal growth factor The growth factor was dissolved in 60 g of water, and then 1 mL of papain (10 U / mL, purchased from Changsha Xiangzi Biotechnology Co., Ltd.) was added to mix. The hydrolysis time was 20 min, heated to 90 ° C, kept for 10 min, and centrifuged at 5000 rpm for 15 min. Add two to six volumes of diethyl ether to the supernatant, and centrifuge at 5000 rpm for 15 minutes after standing still to obtain a precipitate that is a small peptide product after drying.

Embodiment 3

[0050] Embodiment 3: the preparation method of Baiji gel

[0051] Weigh 30 grams of refined Baiji polysaccharide, add 120 grams of water, add 6 grams of acrylic acid and stir for 10 to 30 minutes to obtain a mixture of about 120 mL. Then add 25 μL of 10% ammonium persulfate and 25 μL of TEMED to the mixture, mix quickly and place it at room temperature for 2 hours, then add 300 mL of water and stir, then centrifuge at 8000 rpm, remove the supernatant, add 300 mL of water to the precipitate, stir and then centrifuge at 8000 rpm , to precipitate Ⅵ. The precipitate VI was placed in a vacuum dryer at room temperature and slowly dried to obtain a dried product, namely 35 grams of Baiji gel.

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Abstract

The invention relates to a bletilla striata polysaccharide and paeonol composition product. A water-soluble bletilla striata polysaccharide pure product is obtained by heat-extracting dried bletilla striata by virtue of distilled water, adding neutral protease hydrolyzed protein, filtering, decompressing and concentrating; then, adsorbing by virtue of a column, concentrating an eluent, decompressing and concentrating, wherein the polysaccharide content of the pure product is above 99%. Bletilla striata gel is synthesized from the bletilla striata polysaccharide and acrylic acid, the bletilla striata gel, the bletilla striata polysaccharide and the paeonol are added to water and are promoted to dissolve under the actions of ultrasonic wave and small peptide, the dissolved materials are mixed and are freeze-dried, and then an obtained product is applied to health care products or medicines.

Description

Technical field: [0001] The invention relates to a combination product of Baiji polysaccharide and paeonol and its application in sedative and anti-inflammatory drugs, belonging to the technical field of natural medicine application. Background technique [0002] Paeonol is a traditional Chinese medicine ingredient extracted from peony root bark. It is easily soluble in ethanol and methanol, soluble in ether, acetone, benzene, chloroform and carbon disulfide, slightly soluble in water, and soluble in hot water. Insoluble in cold water, can volatilize with water vapor. It has the effects of sedative, hypnotic, antibacterial, anti-inflammatory, antioxidative, and blood pressure lowering. In terms of daily chemicals, paeonol can inhibit intracellular O 2 - The generation of free radicals can whiten the skin, restore and fade the pigment deposited in the skin, and eliminate stasis and spots; it also has anti-inflammatory, swelling and pain-relieving, anti-allergic, anti-viral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/34A61Q19/02A61Q19/00A61Q19/08
CPCA61K8/347A61K8/73A61K2800/592A61K2800/82A61Q19/00A61Q19/02A61Q19/08
Inventor 向华唐忠海
Owner HUNAN AGRICULTURAL UNIV
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