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Preparation method and application of photoelectrochemical estradiol sensor based on magnetic photosensitive nanomaterial

A photoelectrochemical and photosensitive material technology is applied in the field of preparation of photoelectrochemical estradiol sensors, which can solve the problems of low sensitivity of photoelectrochemical sensors, weakened photoelectric signals, poor conductivity, etc. , prevent the effect of compounding

Inactive Publication Date: 2016-05-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the photogenerated electron-hole pairs of a single titanium dioxide nanomaterial are easy to recombine, which leads to the weakening of the photoelectric signal, and the poor conductivity of titanium dioxide also limits the sensitivity of photoelectrochemical sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 FeCo-NTiO 2 preparation of

[0036] First, take 0.8mmol iron salt, 0.8mmol cobalt salt and 1mmol ammonium salt and add them to 5mL tetrabutyl titanate. During stirring, slowly add 0.5mL hydrofluoric acid, react in the reactor at 160°C for 24 hours, cool After reaching room temperature, centrifuge and wash with ultrapure water and absolute ethanol three times, then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 1°C / min, and calcinate at 480°C for 60min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0037] Described iron salt is ferric sulfate;

[0038] Described cobalt salt is cobalt sulfate;

[0039] Described ammonium salt is ammonium sulfate.

Embodiment 2

[0040] Example 2 FeCo-NTiO 2 preparation of

[0041] First, take 0.8mmol iron salt, 1.0mmol cobalt salt and 1mmol ammonium salt and add them to 5mL tetrabutyl titanate. During the stirring process, slowly add 0.65mL hydrofluoric acid, react in the reactor at 180°C for 21 hours, and cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 2°C / min, and calcinate at 520°C for 30min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0042] Described iron salt is ferric chloride;

[0043] Described cobalt salt is cobalt chloride;

[0044] Described ammonium salt is ammonium chloride.

Embodiment 3

[0045] Example 3 FeCo-NTiO 2 preparation of

[0046] First, add 0.8mmol iron salt, 1.2mmol cobalt salt and 1mmol ammonium salt to 5mL tetrabutyl titanate. During stirring, slowly add 0.8mL hydrofluoric acid, react in the reactor at 200°C for 18 hours, cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 3°C / min, and calcinate at 560°C for 10 min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0047] Described iron salt is ferric nitrate;

[0048] Described cobalt salt is cobalt nitrate;

[0049] Described ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method of a photoelectrochemical estradiol sensor, and belongs to the technical field of novel nano functional materials and biosensors. The method comprises the steps that a novel two-dimensional flaky magnetic photosensitive nanomaterial FeCo-N@TiO2 is prepared firstly, and by means of the good biocompatibility, large specific surface area and ferromagnetism of the material, the material is loaded with an estradiol antibody; alkaline phosphatase is fixed by means of the crosslinking action of glutaraldehyde; when detection is conducted, the alkaline phosphatase can catalyze sodium L-ascorbyl-2-phosphate (APP) to achieve in-situ generation of L-ascorbic acid (AA) to provide electron donors for photoelectric detection, the influence of specific quantitative combination of the antibody and an antigen on electron transmission capability is utilized for enabling the photoelectric current intensity to be correspondingly decreased, and the unmarked photoelectrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare is finally prepared for detecting estradiol.

Description

technical field [0001] The invention relates to a preparation method of a photoelectrochemical estradiol sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Estradiol is a synthetic estrogen that is a steroid. At present, the methods for detecting estradiol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they c...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/305
Inventor 张勇孙旭魏琴李燕马洪敏
Owner UNIV OF JINAN
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