Extraction method of alfalfa dietary fibers and application of alfalfa dietary fibers

A dietary fiber and extraction method technology, which is applied in the field of extraction of alfalfa dietary fiber, can solve the problems of product water holding capacity and swelling power decrease, high processing cost, and reduce the yield of dietary fiber, etc., so as to improve water holding capacity and prolong satiety Effect of time, expansion power improvement of feeling

Inactive Publication Date: 2016-05-25
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the methods of extracting dietary fiber at home and abroad are mainly chemical methods. This process is simple and low in input cost, and has been applied to industrial production. Alkali soaking and repeated rinsing with water not only reduce the yield of dietary fiber, but ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for extracting alfalfa dietary fiber is as follows:

[0021] (1) Remove impurities from the alfalfa, smash it, pass a 50-mesh sieve, defatted with ethyl acetate, add ethyl acetate to the dry alfalfa seeds, the mass ratio of dry alfalfa to ethyl acetate is 1:4, soak for 3 hours After removing ethyl acetate, washing with water, baking at 100°C for 8 hours to obtain defatted alfalfa;

[0022] (2) Add amylase to defatted alfalfa. The amount of amylase is 5% of the weight of defatted alfalfa. Under the conditions of temperature of 95℃ and pH of 4, the hydrolysis is carried out with shaking in a water bath for 8 hours; Add alkaline protease. The amount of protease is 0.5% of the weight of defatted alfalfa. Under the conditions of temperature of 55℃ and pH of 6, the hydrolysis is carried out with shaking in a water bath for 2h; after protease hydrolysis, the enzyme is inactivated at 80℃ for 10min, The obtained mixture is centrifuged to obtain a precipitation layer and a...

Embodiment 2

[0027] The method for extracting alfalfa dietary fiber is as follows:

[0028] (1) Purify the alfalfa after removing impurities and sun drying, pass through a 60 mesh sieve, defatted with ethyl acetate, add ethyl acetate to the dry alfalfa seeds, the mass ratio of dry alfalfa to ethyl acetate is 1:6, soak for 5 hours After removing ethyl acetate, washing with water, baking at 105°C for 12 hours to obtain defatted alfalfa;

[0029] (2) Add amylase to defatted alfalfa. The amount of amylase is 6% of the weight of defatted alfalfa. Under the conditions of temperature of 100°C and pH of 5, hydrolyze with shaking in a water bath for 10 hours; in the mixture after enzymatic hydrolysis with amylase Add papain, the amount of protease is 1% of the weight of defatted alfalfa, under the conditions of temperature 65℃, pH 6.5, water bath shaking for 3h; after proteolysis, at 95℃, the enzyme is inactivated for 20min, the result is Centrifugal separation of the mixture to obtain the precipitatio...

Embodiment 3

[0034] The method for extracting alfalfa dietary fiber is as follows:

[0035] (1) Purify the alfalfa after removing impurities and sun drying, pass through a 60 mesh sieve, defatted with ethyl acetate, add ethyl acetate to the dry alfalfa seeds, the mass ratio of dry alfalfa to ethyl acetate is 1:6, soak for 5 hours After removing ethyl acetate, washing with water, baking at 105°C for 12 hours to obtain defatted alfalfa;

[0036] (2) Add amylase to defatted alfalfa. The amount of amylase is 6% of the weight of defatted alfalfa. Under the conditions of temperature of 100°C and pH of 5, hydrolyze with shaking in a water bath for 10 hours; in the mixture after enzymatic hydrolysis with amylase Add neutral protease, the amount of protease is 1% of the weight of defatted alfalfa, the temperature is 65℃, pH is 6.5, water bath shakes for 2h; after proteolysis, at 80℃, the enzyme is inactivated for 10min, The obtained mixture is centrifuged to obtain a precipitation layer and a supernata...

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PUM

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Abstract

The invention discloses an extraction method of alfalfa dietary fibers. The extraction method comprises the following steps: crushing alfalfas and degreasing with ethyl acetate to obtain degreased alfalfas; adding amylase and protease into the degreased alfalfas; after carrying out water-bath vibration enzymolysis, carrying out enzyme deactivation; separating to obtain a sediment layer and liquid supernatant; freeze-drying the sediment layer to obtain insoluble alfalfa dietary fibers; precipitating the liquid supernatant through absolute ethyl alcohol; and freeze-drying in vacuum and precipitating to obtain soluble alfalfa dietary fibers. The invention further discloses application of the alfalfa dietary fibers used as meal replacement powder. The insoluble alfalfa dietary fibers and the soluble alfalfa dietary fibers are mixed with green tea powder, lotus leaf powder, shrimp meat powder, peanut powder, soybean powder, white kidney bean powder, jumble bead powder and mung bean powder to prepare the meal replacement powder which contains rich proteins, dietary fibers, mineral substances and vitamins, and has a good mouth feel; the satiety time can be obviously prolonged; and the dietary fibers produced by using an enzymolysis method have relatively good sensory quality including color and luster, and flavor and the like.

Description

Technical field [0001] The invention relates to a dietary fiber, in particular to an extraction method and application of alfalfa dietary fiber. Background technique [0002] Alfalfa is a generic name for plants of the genus Medicago, commonly known as "clover" (clover can also be called other trifolium plants), perennial flowering plants, the most famous of which is Medicagosativa (Medicagosativa) as pasture, which is livestock feed. [0003] Each 100g of fresh alfalfa contains 118 mg of vitamin C, 713 mg of calcium, 9.7 mg of iron, and 8.5 mg of selenium. Alfalfa can alkalize the body and detoxify the body. It is used as a diuretic, anti-inflammatory and anti-fungal. Lower cholesterol, balance blood sugar and hormones, and promote pituitary gland function. It is helpful for anemia, arthritis, ulcers, bleeding disorders, bone or joint diseases, digestive system and skin. [0004] Dietary fiber can be divided into two categories: water-soluble fiber (SDF) and water-insoluble fiber...

Claims

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Application Information

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IPC IPC(8): A23L33/21A23F3/14A23L2/52A23C9/156
CPCA23F3/14A23C9/156A23L2/52A23V2002/00A23V2200/332A23V2250/5116A23V2300/28A23V2300/14
Inventor 严学兵郭玉霞郭志鹏张靖雪管永卓陈钊余莹张明
Owner HENAN AGRICULTURAL UNIVERSITY
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