Preparation method for nano polypyrrole chitin nerve conduit

Abstract

The invention provides a preparation method for a nano polypyrrole chitin nerve conduit. The preparation method comprises the following steps: dropwise adding a pyrrole monomer and an initiator into an emulsifier aqueous solution for reaction, adding acetone for stopping the reaction, washing away the initiator and an emulsifier, and separating out precipitates-nano polypyrrole by centrifugation; dissolving nano polypyrrole and chitosan in acetic acid separately to prepare a nano polypyrrole and acetic acid suspension liquid and a chitosan and acetic acid suspension liquid, mixing and stirring to obtain a mixed suspension liquid, injecting the mixed suspension liquid into a mould, performing freeze-drying forming to obtain semi-solid gel, performing neutralization forming in a sodium hydroxide aqueous solution, and drying to obtain a nano polypyrrole chitosan nerve conduit; and soaking the nano polypyrrole chitosan nerve conduit in an acetic anhydride and methanol solution for reaction, soaking a reaction product in a sodium hydroxide solution, performing crosslinking by using genipin, flushing a crosslinked product with double distilled water, and performing dehydrating and drying to obtain the nano polypyrrole chitin nerve conduit. The nano polypyrrole chitin nerve conduit prepared with the method has good conductivity and controllable degradability.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of a nano polypyrrole chitin nerve guide. Background technique [0002] The repair of peripheral nerve defects is one of the difficult problems in clinical treatment. At present, the best recognized treatment method is autologous nerve transplantation. This type of surgery has the disadvantages of limited source of transplant donors, inconsistent diameter of grafted nerves and recipient nerves, and permanent denervation of the donor site. Dysfunction, neuroma formation and many other disadvantages. Therefore, finding a substitute for autologous nerve transplantation that does not damage itself, can be cut according to the length and thickness of the defect, and has a certain curative effect has become the goal pursued by basic and clinical workers. [0003] In recent years, with the emergence and development of tissue engineering, the developme...

AI Technical Summary

Problems solved by technology

[0012] Technical problem to be solved: Aiming at the shortcomings of existing degradable materials such as poor electrical conductivity and difficult biodegradation of conductive materials, the present invention provides a nano-polypyrrole carapace The preparation method of the neuron nerve guide, the prepared nano-polypyrrole chitin nerve guide has good electrical conductivity and controllable degradability