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Skin patch for treating skin burn and preparation method of skin patch

A skin and patch technology, which is applied in the field of skin patches for treating skin burns and their preparation, can solve problems such as the efficacy of immune rejection, and achieve the effects of improving skin repairing effect, repairing skin wounds, and rapidly skin wounds

Inactive Publication Date: 2016-06-01
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is, aiming at defects such as the use of allogeneic skin matrix to repair skin burn wounds in the prior art, which easily causes immune rejection and has low curative effect efficiency, to provide a kind of drug that can avoid immune rejection and has better repairing effect. Skin patch for treating skin burns and preparation method thereof

Method used

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  • Skin patch for treating skin burn and preparation method of skin patch

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preparation example Construction

[0024] Specifically, the preparation method of the skin patch for treating skin burns provided by the present invention comprises the following steps:

[0025] S1. Obtaining fat stem cells and allogeneic skin matrix respectively;

[0026] Among them, the adipose stem cells are derived from the autologous body of patients with skin burns; the allogeneic skin matrix is ​​the skin matrix after decellularization, which can be allogeneic skin matrix (that is, human skin matrix other than skin burn patients), or allogeneic skin matrix. Substrates (such as animal skin substrates such as pigs and cattle);

[0027] S2. The adipose stem cells are mixed with the collagen solution and seeded on the allogeneic skin matrix.

[0028] The allogeneic skin matrix is ​​not only used as a patch for repairing burnt skin, but also retains the insoluble components in the original tissue structure, mainly including collagen, elastin, aminodextran and structural proteins, etc. Restoration provides a...

Embodiment 1

[0054] A kind of skin patch for treating skin burn provided by the present invention, its preparation method comprises the following steps:

[0055] S1a. Patient selection;

[0056] Inclusion criteria for burn patients: patients with second to third degree burn wounds, and the following patients were excluded:

[0057] Pregnant and lactating women; those who are allergic to biological agents used in treatment; severe inhalation injury and other important organ injuries, such as: liver and kidney function injury, craniocerebral injury, etc.; serious basic diseases such as: diabetes, hypertension, tumor or Chronic organ damage, etc.; those who hinder the signing and understanding of informed consent.

[0058] S2a, before treatment, check and evaluate the patient;

[0059] Evaluation criteria: blood routine test (WBC>3.0×10*9 / L, LC>15%; Hb>80g / L; PLT>60×10*9 / L), liver and kidney function, hepatitis B three lines (surface antigen negative ), hepatitis C antibody negative, AIDS ...

Embodiment 2

[0082] The difference from Embodiment 1 is that in this embodiment,

[0083] S3b, the acquisition of fat stem cells;

[0084] S31b, collecting adipose tissue;

[0085] Select patients who meet the evaluation criteria in step S2a, use a liposuction machine to extract adipose tissue from the patient's abdomen, buttocks, thighs and other fat-rich parts under aseptic conditions, remove the blood vessels and fibers that are visible to the naked eye in the fat, and wash the fat with PBS The tissue was put into the buffer solution containing the double antibody (penicillin / streptomycin, each with 1% content) for 3 times, and stored under the condition of 4° C. in a sterile environment for future use.

[0086] S32b, obtaining primary adipose stem cells;

[0087] Take the adipose tissue obtained in step S31b, shred it under sterile conditions, add 0.075% collagenase type Ⅰ, digest at 37°C for 20 minutes on a shaking shaker with a vibration speed of 50 rpm, and then add an equal volum...

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Abstract

The invention relates to a preparation method of a skin patch for treating a skin burn. The preparation method comprises the following steps: respectively obtaining adipose-derived stem cells and allogenic skin matrix; mixing the adipose-derived stem cells with a collagen solution and then inoculating into the allogenic skin matrix, wherein the adipose-derived stem cells are from a patient with the skin burn. By differentiation potential of the adipose-derived stem cells and the stimulation-inducing effect of the allogenic skin matrix, the adipose-derived stem cells are prompted to differentiate into epidermis cells. The adipose-derived stem cells are from the patient with the skin burn, so that the antigenicity is relatively low, and immunological rejection is not easily caused, therefore, the skin repair effect is improved; the target of quickly repairing the skin wound is achieved. In addition, the repairing speed is increased, so that pigment accumulation of the wound part is not easily caused to form a scar and the like; and the pain of the patient is relieved.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a skin patch for treating skin burns and a preparation method thereof. Background technique [0002] The skin is the barrier between the human body and the external environment. It not only plays the roles of protection, secretion, metabolism and sensation, but also participates in the immune response and maintains the stability of the internal environment of the body. As the most exposed skin of the human body, the incidence of injury and large-scale burn accidents is relatively high. Early burn wound coverage can inhibit the growth of bacteria, prevent infection, and prevent the loss of water and electrolytes; autologous skin transplantation is the first choice for the treatment of burns, but for large area burns, autologous skin cannot meet the needs, and the treatment cycle is long. , the skin wound is prone to infection; while the allogeneic skin matrix will cause obvious ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/60A61L27/24
CPCA61L27/362A61L27/24A61L27/3691A61L27/3834A61L27/60A61L2400/18
Inventor 曾宪卓张哲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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