Method for preparing polysaccharide selenite and application thereof
A technology of selenite and selenate, which is applied in the direction of medical preparations containing active ingredients, anti-toxic agents, organic active ingredients, etc., can solve the cumbersome preparation, low selenium content of polysaccharide selenite, low selenium content, etc. Problems, to achieve a wide range of physiological functions, product yield and high quality effect
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Embodiment 1
[0025] Example 1MTT method in vitro detection of polysaccharide selenite anticancer drug sensitivity test:
[0026] Cells in the logarithmic growth phase were diluted to 2.0×106 cells mL-1 with RPMI-1640 medium containing 10% fetal bovine serum, inoculated in a 96-well plate, 100 μL per well, and then added the drug to be tested and the negative control in sequence group, positive control group, and keep the blank group with medium only. Subsequently, culture was continued for 24 hours in an incubator at 37° C. and 5% CO 2 , and then 10 μL of 5 mg·mL −1 MTT solution was added to each well, and culture was continued for 4 hours. Aspirate the supernatant, add 100 μL of DMSO to each well, and measure the absorbance (A) value with a microplate reader at a wavelength of 570 nm. Calculate the cell proliferation inhibition rate with the following formula, and use the formula T / C (%)=100×A 阴性 / A 样品 The half inhibitory concentration (IC50) inhibitory rate=(A 空白 -A 给药 ) / A 空白
[0...
Embodiment 2
[0028] Embodiment 2 antioxidant activity research
[0029] 1. to O 2 · The scavenging effect
[0030] Contains polysaccharide selenite (1mg·mL -1 ) Tris-HCl buffer solution of pH 8.2 was incubated at 25°C for 20 minutes, and 0.1mol·L -1 10mmol·L of pyrogallol -1 Hydrochloric acid solution, shake quickly, measure the absorbance at 319nm at 25°C, and continuously measure A p .
[0031] Do not add polysaccharide selenite, operate as above, measure its contrast absorbance value A s . The test showed that the absorbance A p Relative Absorbance A s reduce.
[0032] The results showed that: polysaccharide selenite can effectively scavenge active oxygen.
[0033] 2. Scavenging effect on hydroxyl radicals and protecting DNA
[0034] Using the Fenton system, take 0.2mL FeSO4-EDTA mixed solution (10mmol L -1 ) into the test tube, add 0.5mL of DNA (10mmol·L -1 ), then add polysaccharide selenite (0.8mg·mL -1 ), with phosphate buffer (pH7.4, 0.1mol L -1 ) to 1.8ML, add 0.2mL...
Embodiment 3
[0037] Embodiment 3 dextrin selenite
[0038] Under stirring, 1g dextrin, 0.1g CH3 OOS 2 h 5 and 25g selenium oxyfluoride (SeOF 2 ) into pyridine, heated to 100°C, reacted for 24 hours, and added water. The reaction solution was lowered to room temperature, and ethanol was added to the reaction solution according to 3 times the volume of the reaction solution, and stirred; filtered, then washed with ethanol, and dried the filtrate; dissolved the filtrate, and adjusted the pH of the solution with NaOH solution 7 to 8, then add ethanol, filter, then wash the filtrate with ethanol, and dry the product. The product is refined by dialysis. Dextrin selenite containing 45% selenium was prepared.
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