Field planting method for pleurotus tuber-regium

A planting method, the technology of tiger milk mushroom, applied in the biological field, can solve the problems of sclerotia growth scale, different performance, low yield of strain cultivation, difficult large-scale production capacity, etc., and achieve low price, low cost and high formula The effect of innovation

Inactive Publication Date: 2016-06-22
WUZHOU LINCHUAN JINSHAN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production method of prior art tiger milk mushroom sclerotium is to obtain bacterial classification by the tissue separation of sclerotium or sporocarp, and culture with fixed medium, the ubiquitous bacterial classification cultivation time is long, and bacterial classification cultivation yield is low, and growth period is long, The problem of small sclerotium and low yield, and because of the different tissues used, it is easy to cause the growth scale and performance of sclerotia to be different, and long-term use will lead to the degradation of the production shape
Therefore, it is difficult for existing technologies to form large-scale production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The collection of embodiment 1 tiger's milk mushroom and the separation of biological clone cell tissue

[0025] During the fruiting season of wild tiger milk mushrooms, fresh tiger milk mushrooms with large flowers, thick flesh, robust, no pests and diseases, and in the prime of life are collected for biological cloning and cell tissue separation. The PDA medium used for separation is: 50 grams of camphor wood slices, 100 grams of lotus wood slices, 22 grams of glucose, 22 grams of agar, 1.5 grams of potassium dihydrogen phosphate, 3 grams of magnesium sulfate, 11.5 grams of VB, 1000 milliliters of water.

[0026] Cook the camphor wood slices and lotus wood slices together for 30 minutes, filter to get the juice, add the above raw materials and cook until they melt, then pack into test tubes, seal and sterilize. After maintaining the pressure of 1.5 pounds in an autoclave for 60 minutes, put it on a slope while it is still hot, preferably with a slope of 2 / 3 of the tes...

Embodiment 2

[0028] The making and cultivation of the original species of tiger milk mushroom of embodiment 2

[0029] Raw material and formula: wheat 50-60%, camphor wood 5-10%, lotus wood 10-20%, wheat bran 10-15%, gypsum 1%, superphosphate 1-2%, water content 60-65% , put into a glass bottle of 750 grams, seal and sterilize, and keep the temperature at 120°C for 2-3 hours or at 100°C for 8-10 hours. Inoculate after cooling.

[0030] The inoculation method is carried out in the inoculation box or under sterile conditions. First put the sterilized original seed medium into the inoculation box, and after disinfecting the inoculation needle and the mother seed of tiger milk mushroom with 75% alcohol, put it into the inoculation box. In the box, 5 grams of potassium permanganate and 10 milliliters of formaldehyde are mixed to generate gas for space disinfection, and the inoculation starts after 30 minutes.

[0031] Each mother seed can be connected with 5-8 bottles of original seed, placed...

Embodiment 3

[0032] The making of embodiment 3 milk mushroom cultivars

[0033] Raw materials and formula: 50-60% of plant organic matter or straw powder, 5-10% of camphor wood, 10-20% of lotus wood, 10-15% of wheat or rice bran, 1% of gypsum, 1% of lime, water content of 60% -65%, put it into a 17×33cm plastic bag, seal it, and sterilize it. Keep the normal pressure above 100°C for 8 hours, and keep the high pressure at 1.5 pounds for 90 minutes, and inoculate after cooling. Each bag of original seeds can be inoculated with 50-80 bottles of cultivars. After inoculation, they are placed in an environment of 20-30°C for cultivation. The whole bag can be cultivated after 20-30 days of mycelia.

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Abstract

The invention discloses a field planting method for pleurotus tuber-regium. The field planting method includes the steps that mother cultures of pleurotus tuber-regium are cultivated, raw cultures of the pleurotus tuber-regium are made, cultivable cultures of the pleurotus tuber-regium are cultivated, and cultivable pleurotus tuber-regium cultures are obtained in the mode; then a flat paddy field is selected for seeding, the shade degree is kept 70%-80%, the soil water content is kept 60%-65%, the relative air humidity is kept 80%-95%, and the temperature is kept within 25-35 DEG C till the pleurotus tuber-regium is grown and harvested. The field planting method has the advantages of being wide in material, low in price and production cost, simple in working procedure, high in yield, large in income and good in quality, and products the same as wild pleurotus tuber-regium can be cultivated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a field-based planting method of tiger milk mushroom. Background technique [0002] Tiger milk mushroom sclerotium is a tropical edible fungus, mainly produced in Africa, and also distributed in tropical Asia and Australia. There is a tradition of eating in some countries and regions, and it can also be used as medicine in the folk: it is used to treat stomach pain, constipation, fever, cold, edema, chest pain, nervous system diseases, smallpox, asthma, high blood pressure, diabetes and other diseases, and can promote fetal health. development and improve the survival rate of premature infants. With the improvement of people's living standards and the wide application of medicine, the demand for tiger milk mushroom will be increasing, and the wild tiger milk mushroom can not meet people's needs far away. In recent years, there have been reports of artificial cultivation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
CPCA01G18/00C05B1/02C05D3/00C05F5/002C05F11/00
Inventor 方金山江国志方婷
Owner WUZHOU LINCHUAN JINSHAN BIOLOGICAL TECH CO LTD
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