Preparation method of biological amniotic membrane easy to preserve

A technology of biological amnion and amniotic membrane, which is applied in the field of preparation of easy-to-preserve biological amniotic membrane, can solve the problems of inconvenient use, poor mechanical strength, and difficult operation, and achieve the effect of mild reaction, high mechanical strength, and simple operation process

Active Publication Date: 2016-08-10
湖州菱创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: in view of the current situation that fresh amnion commonly used is not conducive to preservation and inconvenient to use, and the material of amnion is thin, has poor mechanical strength and is easy to tear, which leads to difficult operation during surgery. A kind of enzymatic hydrolysis of amniotic membrane, after filtering, adding glycerin to soak, and washing with physiological saline to obtain crude biological amniotic membrane, then take chitosan and sterile water, mix and add phosphate buffer solution, tea polyphenols, casein and ammonium persulfate , mix and heat again and add chitin to obtain a mixed solution, then soak the obtained crude amniotic membrane in the mixed solution, react with microwaves, wash and filter, and place it in a sterile purification room to dry naturally, so as to obtain the preparation of easy-to-preserve biological amniotic membrane method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in 4% lactic acid solution, put it in an ultrasonic oscillator, vibrate at 23KHz for 50min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluate is 7.0, mix the amniotic membrane with 3% trypsin in the quality of the amniotic membrane after washing, stir evenly, and enzymolyze it at 30°C for 5 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 4°C, then filter, rinse the filtered amnion 5 times with normal sali...

example 2

[0020]First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in 5% lactic acid solution, put it in an ultrasonic oscillator, vibrate at 24KHz for 60min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluate is 7.0, mix the amniotic membrane and amniotic membrane quality 4% trypsin after washing, stir evenly, and enzymolyze at 33°C for 6 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 5°C, then filter, rinse the filtered amnion 6 times with normal saline to obtain crude ...

example 3

[0023] First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in a lactic acid solution with a mass fraction of 6%, put it in an ultrasonic oscillator, vibrate at 25KHz for 70min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluent is 7.0, mix the amniotic membrane with 5% trypsin in the quality of the amniotic membrane after washing, stir evenly, and enzymolyze it at 35°C for 7 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 6°C, then filter, wash the filtered amnion...

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Abstract

The invention discloses a preparation method of a biological amniotic membrane easy to preserve and belongs to the field of biological amniotic membrane preparation. The preparation method comprises the steps that an amniotic membrane undergoes cleaning and enzymolysis, glycerol is added for soaking after filtering, flushing is performed with physiological saline to obtain a coarse biological amniotic membrane, then chitosan and sterile water are taken and mixed, a phosphoric acid solution, tea polyphenol, casein and ammonium persulfate are added, re-mixing and heating are performed, chitin is added to obtain a mixed solution, and then the coarse biological amniotic membrane is socked in the mixed solution, undergoes microwave reaction, flushing and filtration and then is put in a sterile purification room for natural air drying so as to obtain the biological amniotic membrane easy to preserve. Examples prove that the preparation method is simple and convenient and does not need special equipment, and the defects of smaller thickness, poor mechanical strength and easy tear of an existing amniotic membrane material are overcome. In addition, the prepared biological amniotic membrane is free of biological toxicity, has better tissue compatibility, can be preserved at room temperature for a long period of time, is convenient to use and can be produced in a large-scale mode, and the preservation time is prolonged to be 3-5 years.

Description

technical field [0001] The invention discloses a method for preparing an easy-to-preserve biological amniotic membrane, which belongs to the field of biological amniotic membrane preparation. Background technique [0002] Due to its unique characteristics, amniotic membrane has attracted the attention of scholars at home and abroad. Amniotic membrane refers to the basement membrane layer of human placenta, which is a collagen tissue layer without blood vessels and cell structure. It is rich in type IV and V collagen and basic fibroblast growth factor. (BFGF), stem cell growth factor (HGF), easy to make epithelial cells migrate into, promote epithelial differentiation and proliferation, and play an important role in epithelial wound healing. Amniotic membrane contains various protein inhibitors, which play an anti-inflammatory effect by inhibiting the corresponding proteases. At the same time, it also contains TGF-β, which inhibits the expression of cytokines and regulates a...

Claims

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Application Information

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IPC IPC(8): A61L31/00A61L31/14A01N1/02
CPCA01N1/0205A01N1/0215A01N1/0226A01N1/0231A61L31/005A61L31/14
Inventor 郭迎庆张明宋奇
Owner 湖州菱创科技有限公司
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