Preparation method of biological amniotic membrane easy to preserve
A technology of biological amnion and amniotic membrane, which is applied in the field of preparation of easy-to-preserve biological amniotic membrane, can solve the problems of inconvenient use, poor mechanical strength, and difficult operation, and achieve the effect of mild reaction, high mechanical strength, and simple operation process
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example 1
[0017] First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in 4% lactic acid solution, put it in an ultrasonic oscillator, vibrate at 23KHz for 50min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluate is 7.0, mix the amniotic membrane with 3% trypsin in the quality of the amniotic membrane after washing, stir evenly, and enzymolyze it at 30°C for 5 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 4°C, then filter, rinse the filtered amnion 5 times with normal sali...
example 2
[0020]First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in 5% lactic acid solution, put it in an ultrasonic oscillator, vibrate at 24KHz for 60min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluate is 7.0, mix the amniotic membrane and amniotic membrane quality 4% trypsin after washing, stir evenly, and enzymolyze at 33°C for 6 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 5°C, then filter, rinse the filtered amnion 6 times with normal saline to obtain crude ...
example 3
[0023] First take fresh amniotic membrane, clean its surface with water, soak the cleaned amniotic membrane in a lactic acid solution with a mass fraction of 6%, put it in an ultrasonic oscillator, vibrate at 25KHz for 70min, filter it, and rinse it with distilled water On the surface of the amniotic membrane, until the pH of the eluent is 7.0, mix the amniotic membrane with 5% trypsin in the quality of the amniotic membrane after washing, stir evenly, and enzymolyze it at 35°C for 7 hours; after the above enzymatic hydrolysis is completed, filter and collect the amniotic membrane , spread the Dimembrane on the surface of ice cubes, spread the enzymatically hydrolyzed amnion on the surface of the Dimembrane, with the subcutaneous side of the amnion facing down, use a cell scraper to remove the surface impurities on the amnion, and then place the amnion after the removal of impurities Soak in glycerin, stand for dehydration overnight at 6°C, then filter, wash the filtered amnion...
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