Biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, method for preparing biological nanometer material and application thereof

A bio-nanomaterial, seawater psychrophilic technology, applied in biochemical equipment and methods, microorganism-based methods, nanostructure manufacturing, etc., can solve problems such as difficult processing, and achieve good film formation and particle size distribution. Uniform, low-cost effect

Active Publication Date: 2016-08-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its high phosphorus and high salinity, s...

Method used

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  • Biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, method for preparing biological nanometer material and application thereof
  • Biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, method for preparing biological nanometer material and application thereof
  • Biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, method for preparing biological nanometer material and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1 Screening of bacterial strain Psychrobacter aquimaris X3-1403 according to the present invention

[0048] 1. Screening process

[0049] ⑴Cultivate 464 strains from the South China Sea in seawater LB liquid medium for 24 hours (200rpm, 25°C), and observe whether self-flocculation particles are formed during the shaking culture process.

[0050] (2) Centrifuge the strain capable of self-flocculation during shaking culture at 5000rpm for 10 minutes, discard the supernatant, wash the bacteria twice with deionized water, and then observe whether there are nano-sized particles on the surface of the bacteria under a transmission electron microscope.

[0051] (3) The above-screened strains that can self-flocculate during shaking culture and produce nano-sized particles on the surface of the bacteria are observed under an optical microscope to see if the strains have self-assembly phenomenon.

[0052] (4) The screened bacterial strain capable of self-flocculation and ...

Embodiment 2

[0083] Application of the bacterial strain of the present invention in the treatment of phosphorous salt water of embodiment 2

[0084] After culturing Psychrobacter aquimaris X3-1403 in LB for 24 hours, inoculate 10% of the inoculum into simulated seawater flushing wastewater, and take samples according to time points to determine the strain’s effect on TP, COD, NH 4 + - Removal effect of N and TN. From image 3 It can be seen that the strain has a high removal effect on TP and COD, the removal rates are 70.5% and 75.5% respectively, and the removal rates of 48h are 0.57mg / (L·h) and 18.7mg / (L·h ). And the bacteria to NH 4 + -N and TN have poorer removal effects, only 17.8% and 19.4%.

Embodiment 3

[0085] Example 3 Application of the bacterial strain of the present invention in the preparation of nanomaterial nano-hydroxyapatite

[0086] The strain Psychrobacter aquimaris X3-1403 of the present invention was activated in LB medium for 24 hours, and the culture conditions were 200 rpm and 25°C. Then ①take 25mL of activated culture solution in a pre-sterilized centrifuge tube in the aseptic operating table, centrifuge at 10000rpm for 10min; ③Repeat step ② once. The resuspended bacterial solution was inoculated into simulated seawater toilet flushing wastewater and simulated domestic wastewater (10% inoculum size) respectively, and cultured at 200 rpm and 25° C. for 48 hours. The culture solution was centrifuged at 5000 rpm for 10 min, the supernatant was discarded, and the bacteria at the bottom of the centrifuge tube were washed twice with deionized water to obtain the bacteria containing nanomaterials. A part of the cells were resuspended, fixed and stained on a copper...

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Abstract

The invention discloses a biological nanometer material for synthesizing and automatically assembling psychrobacter aquimaris, a method for preparing the biological nanometer material and application thereof. The method includes steps of carrying out contact culture on the psychrobacter aquimaris X3-1403 by the aid of culture media which at least contain calcium, phosphorus, oxygen and hydrogen elements; centrifugally removing supernatant to obtain the biological nanometer material after contact culture is carried out. The psychrobacter aquimaris X3-1403 belongs to psychrobacter and is already preserved at the China Center for Type Culture Collection in Luojia Hill of Wuchang of Wuhan City on 29 March, 2016, and a preservation number of the psychrobacter aquimaris is CCTCC M 201655. The biological nanometer material, the method and the application have the advantages that psychrobacter aquimaris culture procedures are nanometer hydroxyapatite and biological nanometer material synthesis procedures, conditions are mild during culture, the biological nanometer material is easy to operate, free of pollution, low in cost and high in efficiency and is clean, and the biological nanometer material and the method can be popularized and applied on a large scale.

Description

technical field [0001] The invention relates to a bionano material synthesized and self-assembled by seawater psychrophilic bacilli, a preparation method and application thereof. Background technique [0002] Nanomaterials refer to materials in which at least one dimension of three-dimensional materials is in the nanoscale range (1-100nm) or composed of them as basic units. Nanomaterials have some unique characteristics such as surface effects, small size effects, and quantum effects, so they are widely used in industries such as environmental protection, energy, catalysis, biosensors, and biomedicine. [0003] The traditional physical and chemical synthesis methods of nanomaterials mainly include two-phase method, reverse microemulsion method, photochemical synthesis method, electrode electrolysis method, heating method, ultrasonic method, etc., but these traditional synthesis methods have various insurmountable defects. For example, the price of raw materials is high, the...

Claims

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Application Information

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IPC IPC(8): B82B3/00B82B1/00B82Y30/00B82Y40/00B82Y5/00C12N1/20C12R1/01
CPCB82B1/001B82B3/0019B82Y5/00B82Y30/00B82Y40/00C12N1/20
Inventor 周维芝黄兆松刘婷
Owner SHANDONG UNIV
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