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Soybean protein isolate-based biomedical adhesive and preparation method thereof

A soybean protein isolate, biomedical technology, applied in the field of soybean protein isolate-based biomedical adhesives and its preparation, can solve the problems of cytotoxicity, calcification, dissemination and allergic reactions, and achieve the promotion of induced tissue regeneration and enhanced stability and adhesion, effect of promoting blood coagulation

Inactive Publication Date: 2016-09-21
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of fibrin glue may cause the spread of some blood-borne infectious diseases and allergic reactions; Can cause cytotoxicity and calcification
Therefore, there are few commercial tissue adhesives currently on the market that can be widely used in moist environments in vivo and in vitro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dissolve 100 parts by mass of soybean protein isolate in a phosphate buffer solution with pH=7.4 at 60°C, and then add 25 parts by mass of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride and 10 parts by mass of N-hydroxysuccinimide, adjust the pH value of the reaction system to 5, stir and react at 25°C for 40 minutes, then add 50 parts by mass of dopamine, maintain the pH value of the reaction system at 6, 30°C Continue to stir and react for 24 hours under the same conditions, and finally dialyze and freeze-dry to obtain dopamine-modified soybean protein isolate; when in use, dissolve 100 parts by mass of dopamine-modified soybean protein isolate in a phosphate buffer solution with pH=7.4, and then add 15 parts by mass of Parts of genipin, 0.15 parts by mass of tetracycline hydrochloride and amoxicillin, mixed evenly and spread on the wound surface, then dripping 15 parts by mass of FeCl with a concentration of 0.1M 3 Solution, gently press the wound, and t...

Embodiment 2

[0021] Dissolve 100 parts by mass of soybean protein isolate in a phosphate buffer solution with pH=7.4 at 70°C, and then add 20 parts by mass of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride and 15 parts by mass of N-hydroxysuccinimide, adjust the pH value of the reaction system to 4, stir and react at 30°C for 60 minutes, then add 40 parts by mass of dopamine, maintain the pH value of the reaction system at 6, 35°C Stirring reaction was continued for 18 hours under the conditions, and finally dialysis and freeze-drying were performed to obtain dopamine-modified soybean protein isolate; when used, 100 parts by mass of dopamine-modified soybean protein isolate was dissolved in phosphate buffer solution with pH=7.4, and then 30 parts by mass of Parts of dialdehyde xanthan gum, 0.05 parts by mass of gentamycin, mixed evenly and spread on the wound, then dripping 15 parts by mass of FeCl with a concentration of 0.15M 3 Solution, gently press the wound, and the wound...

Embodiment 3

[0023] Dissolve 100 parts by mass of soybean protein isolate in a phosphate buffer solution with pH=7.4 at 50°C, and then add 30 parts by mass of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride and 15 parts by mass of N-hydroxysuccinimide, adjust the pH value of the reaction system to 7, stir and react at 40°C for 40 minutes, then add 60 parts by mass of dopamine, maintain the pH value of the reaction system at 5, 30°C Continue to stir and react for 30 hours under the same conditions, and finally dialyze and freeze-dry to obtain dopamine-modified soybean protein isolate; when in use, dissolve 100 parts by mass of dopamine-modified soybean protein isolate in a phosphate buffer solution with pH=7.4, and then add 25 parts by mass of Two parts of dialdehyde pullulan and tea polyphenols, 0.2 parts by mass of levofloxacin hydrochloride, mixed evenly, spread on the wound, and then dripping 20 parts by mass of FeCl with a concentration of 0.08M 3 Solution, gently press the...

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PUM

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Abstract

The invention relates to a soybean protein isolate-based biomedical adhesive and a preparation method thereof. The method comprises the following steps: firstly, dissolving soybean protein isolate into phosphate buffer; then, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide; stirring for reacting for 30-60 minutes, and then adding dopamine; continuously stirring for reacting for 10-36 hours; lastly, performing dialysis, centrifugal separation and freeze drying to obtain dopamine-modified soybean protein isolate; during use, the following steps are adopted: dissolving the dopamine-modified soybean protein isolate into the phosphate buffer; then, adding a cross-linking agent and an anti-inflammation anti-bacterial agent; mixing uniformly, and smearing the mixture on a wound; dropwise adding an FeCl3 solution, so that the wound can be bonded firmly within 20-120s. The prepared biomedical adhesive has the characteristics of rapid response and long-acting bonding, can achieve a very good bonding effect in in-vivo and in-vitro wet environments, has high biocompatibility, is fully biodegradable in vivo, and can promote rapid healing of the wound.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a soy protein isolate-based biomedical adhesive with strong adhesive force, good biocompatibility, complete biodegradability and convenient use in wet environments inside and outside the body and its preparation method. Background technique [0002] The concept of modern medicine is not only to achieve the fastest recovery of the patient on the basis of minimizing the pain of the patient, but also hope that its appearance can be restored perfectly. Therefore, the continuous increase of clinical demand has promoted the development of medical adhesive research and the clinical application of adhesive technology. Around 3000 BC, the ancient Egyptians first used a mixture with the properties of a medical adhesive, and later the ancient Greeks used olive oil, lead oxide and water to make a medical plaster-like mixture. However, it was not until recent decades that medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08
CPCA61L24/0015A61L24/0042A61L24/08A61L24/108A61L2300/404A61L2300/41C08L89/00
Inventor 穆畅道葛黎明徐永斌朱明津熊佳容徐锦芳
Owner SICHUAN UNIV
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