Crypthecodinium cohnii and application thereof
A technology of Cryptodinium koii and microbial strains, applied in the direction of single-cell algae, microorganisms, biochemical equipment and methods, etc., can solve the problems of uncultivated large-scale cultivation, achieve good industrial application value, and shorten the fermentation time , the effect of fast growth
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Embodiment 1
[0024] Crypthecodinium cohnii, its taxonomic name is Crypthecodinium cohnii, and the laboratory named Crypthecodinium cohnii SD401 strain, which was preserved in Beichen West Road 1, Chaoyang District, Beijing on April 13, 2016. The General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC) in No. 3 Courtyard, and its deposit number is CGMCC No: 12239.
[0025] The Cryptidium kouli is obtained from the rotted leaves collected in the mangrove area of Shuidongwan, Dianbai, Guangdong, by the pine pollen fishing method. After culturing, observe the morphology and structure under an optical microscope (Figure 1): SD401 cells are ellipsoidal, with a diameter of 8-20 microns, and there are obvious granular substances in the cells. The cells mainly reproduce by division.
[0026] Through cultivation, oil was extracted for detection of fatty acid composition. It can be seen from the results that the main long-chain polyunsaturated fat...
Embodiment 2
[0031] Effects of Different Carbon Sources on the Growth and Oil Accumulation of Cryptidinium koirii SD401 Strain
[0032] In a 250ml Erlenmeyer shaker flask, configure 50ml of medium, the nitrogen source is yeast extract 20g / L, the salinity is 15, add different carbon sources (glycerol, glucose, fructose, xylose, sucrose, maltose and starch) , the concentration is 60g / L, the pH value is adjusted to 6.0, after autoclaving, insert 5ml of the pre-cultivated strain seed solution, and vibrate and cultivate on an air bath shaker at 30°C for 120 hours, and the shaking speed is 200rpm. The bacteria were collected by centrifugation, freeze-dried to constant weight, and the dry weight was measured; some of the bacteria were taken, and the oil was extracted and methylated according to the conventional chloroform-methanol method, and the percentage content of DHA in the bacteria was determined by GC-MS. The results are shown in Table 2.
[0033] Table 2 Effects of different carbon sourc...
Embodiment 3
[0037] Effects of Different Nitrogen Sources on the Growth and Oil Accumulation of Cryptidinium koulii SD401 Strain
[0038]In a 250ml Erlenmeyer shaker flask, configure a 50m culture medium, use glucose as a carbon source, a concentration of 60g / L, and a salinity of 15, add organic nitrogen sources (yeast extract, peptone and tryptone) with a concentration of 20g / L respectively and 5g / L inorganic nitrogen source (urea, ammonium acetate and sodium nitrate), adjust the pH value to 6.0, after autoclaving, insert 5ml of pre-cultivated algae seed liquid, and vibrate at 35°C on an air bath shaker 90 hours, the shaking speed is 180rpm. The analysis method is the same as above, and the experimental results are shown in Table 3.
[0039] Table 3 Effects of different nitrogen sources on the growth and oil accumulation of Cryptidium koii SD401 strain
[0040]
[0041] It can be seen from Table 3 that yeast extract and peptone can better promote the growth and oil accumulation of SD...
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