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Industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate

A technology of Bacillus cereus and liquid fermentation, which is applied in the field of microbial preparations and can solve problems such as unseen industrial production products

Active Publication Date: 2016-12-07
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because not all strains of Bacillus cereus have the characteristics of dissolving dinoflagellates in ponds, and the algicidal bacteria in the above research are not Bacillus cereus or similar algicidal strains in this application, and there is no industrialization of related bacterial agents product

Method used

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  • Industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate
  • Industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate
  • Industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate

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Experimental program
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Effect test

Embodiment 1

[0035] The Establishment of Industrialized Liquid Fermentation Parameters of Bacteria

[0036] Using the 50L fermenter system platform to study and establish the process parameters of the industrialized liquid fermentation of bacterial agents. The growth rate of Bacillus cereus JZBC1, the rate of spore formation, and the algae-lysis efficiency of the fermentation agent were used as evaluation indicators to establish and optimize the fermentation parameters. The obtained relevant fermentation parameters can be applied to the 50L-500L-5000L step-by-step fermentation system platform to carry out the industrialized expanded fermentation culture of the JZBC1 strain.

[0037] First, using the single factor test method, on the basis of the basic fermentation medium, different carbon sources (sucrose, glucose + sucrose, soluble starch, molasses, corn steep liquor, bran, bran + molasses, soluble starch + corn steep liquor) were selected. ) to replace the glucose in the basal medium in...

Embodiment 2

[0048] The industrialized liquid fermentation production of Bacillus cereus JZBC1 was carried out in the Huadu Microbial Industrial Fermentation Experimental Plant of the South China Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences in Huadu District, Guangzhou City. The fermentation system is an industrialized liquid fermentation system of 50L-500L-5000L.

[0049] (1) The Bacillus cereus JZBC1 strain was activated with a nutrient broth medium liquid medium to prepare a seed solution, pH 6.00-7.50, 200-240 rpm, 28-32°C constant temperature shaking culture for 20-30 hours.

[0050] (2) The formulation of the fermentation medium contains 20g of corn steep liquor, 13.5g of soybean protein, 1g of NaCl, and KH in 1L of water. 2 PO 4 0.2g, MgSO 4 0.2g, CaCl 2 0.1g, MnSO with a mass percentage of 3% 4 Solution 1mL, prepare the required culture medium in the fermenter according to the above formula by 70% liquid volume.

[0051] (3) Turn on the heating ...

Embodiment 3

[0057] Different from Example 2, the formula of the fermentation medium is: 1L water contains corn steep liquor 18.5g, soybean protein 0g, NaCl 0.8g, KH 2 PO 4 0.4g, MgSO 4 0.15g, CaCl 2 0.3g, MnSO with a mass percentage of 3% 4 Solution 1 mL.

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Abstract

The invention discloses an industrial liquid fermentation method of bacillus cereus JZBC1 for dissolving pond dinoflagellate. The method comprises the following steps of (1) determination of a fermentation tank culture and fermentation culture medium of the bacillus cereus JZBC1; (2) seed liquid culture: the bacillus cereus JZBC1 is selected for performing activating culture to obtain fermentation seed liquid; (3) industrial fermentation tank culture: the fermentation seed liquid is inoculated into the fermentation culture medium for gradual expanding fermentation according to the 50L-500L-5000L fermentation tank scale to obtain fermentation liquid; (4) bacterial sludge post-treatment and package: the excessive moisture in the fermentation liquid is removed by a centrifugal machine; bacterial sludge is obtained; the bacterial sludge and carriers are uniformly mixed; after the drying, crushing and package work procedure post-treatment, a bacillus cereus JZBC1 preparation is obtained. The method has the advantage that by studying the fermentation parameters and the fermentation culture medium of the bacillus cereus JZBC1, the relevant process applying the microbial inoculums from the bacterial strain to the product industrial mass production is built.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to an industrial liquid fermentation method for dissolving pond dinoflagellate bacillus cereus JZBC1. Background technique [0002] In recent years, dinoflagellate red tides have occurred frequently in the water environment, and the pollution of the aquaculture water environment has deteriorated day by day, which has seriously affected the healthy development of pond aquaculture production. Studies have shown that when the formation of pond water is dominated by harmful microalgae such as dinoflagellates and cyanobacteria, the quality of the aquaculture water will deteriorate, and the growth and health of cultured prawns will be seriously affected (Liu Xiaozhu, 2011); when the concentration of dinoflagellates increases to 10 4 cells / L, causing the shrimp to behave abnormally and gradually die (Linares, 2009). However, in southern seawater or brackish wate...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P13/02C12R1/085
CPCA01N63/00C12N1/20
Inventor 曹煜成文国樑李卓佳胡晓娟李莎莎杨铿徐创文
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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