Method for preparing triphosadenine by enzymic method
An enzymatic preparation technology of adenosine triphosphate, which is applied in the field of enzymatic preparation of adenosine triphosphate, can solve problems such as unclear classification, and achieve the effects of easy control, simple production process, and cost reduction
Active Publication Date: 2016-12-07
BEIJING TIANKAI YIDA BIOLOGICAL SCI & TECH
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Abstract
The invention discloses a method for preparing triphosadenine by an enzymic method. The method comprises the following steps of (1) preparing ATP production enzymes: obtaining the ATP production enzymes in gene engineering transformation, fermentation, purification or direct extraction modes; (2) preparing ATP in reaction liquid; adding adenosine, the ATP production enzymes and polyphosphoric acid or salts of the polyphosphoric acid for ATP preparation; (3) separating products and ATP production enzymes; directly separating immobilized ATP production enzymes in a reaction tank, and separating free ATP production enzymes through an ultrafiltration membrane in a filtering device; (4) preparing a finished product: preparing the reaction liquid subjected to the enzyme separation into finished product ATP through the steps of ion-exchange column chromatography, concentration, crystallization; drying and the like. The method has the advantages that the novel ATP production enzymes are used; the production process is simple; the control is easy; the adenosine is used as a substrate; the cost is reduced; an APT production enzyme recovery system is built; the method is applicable to industrial production; the content of impurities such as impurity protein and pigment in the enzymic method preparation reaction liquid is low; the subsequent purification is easy.
Application Domain
Fermentation
Technology Topic
Ion exchangeUltrafiltration +10
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