Method for preparing triphosadenine by enzymic method

Abstract

The invention discloses a method for preparing triphosadenine by an enzymic method. The method comprises the following steps of (1) preparing ATP production enzymes: obtaining the ATP production enzymes in gene engineering transformation, fermentation, purification or direct extraction modes; (2) preparing ATP in reaction liquid; adding adenosine, the ATP production enzymes and polyphosphoric acid or salts of the polyphosphoric acid for ATP preparation; (3) separating products and ATP production enzymes; directly separating immobilized ATP production enzymes in a reaction tank, and separating free ATP production enzymes through an ultrafiltration membrane in a filtering device; (4) preparing a finished product: preparing the reaction liquid subjected to the enzyme separation into finished product ATP through the steps of ion-exchange column chromatography, concentration, crystallization; drying and the like. The method has the advantages that the novel ATP production enzymes are used; the production process is simple; the control is easy; the adenosine is used as a substrate; the cost is reduced; an APT production enzyme recovery system is built; the method is applicable to industrial production; the content of impurities such as impurity protein and pigment in the enzymic method preparation reaction liquid is low; the subsequent purification is easy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for enzymatically preparing adenosine triphosphate. Background technique [0002] Adenosine triphosphate (ATP) consists of one adenosine (A) and three phosphate groups, with a molecular weight of 507 and molecular formula C 10 h 16 N 5 o 13 P 3 . It is the energy converter and storage device in the living body, and plays an important role in the energy metabolism of the human body. As a metabolic intermediate and coenzyme, it participates in the metabolism of carbohydrates, proteins, nucleic acids and fats in the living body. In clinical application, ATP has a wide range of functions, and has good therapeutic and adjuvant therapeutic effects on progressive muscular atrophy, stroke sequelae, myocardial infarction, myocarditis, coronary arteriosclerosis, hepatitis and other diseases. [0003] The synthesis of ATP mainly includes chemical synthesis, biological enzyme cata...

AI Technical Summary

Problems solved by technology

"ATP-producing enzymes" used include polyphosphate kinase (EC 2.7.4.1, Ppk), adenylate kinase (EC 2.7.4.3, Adk) and polyphosphate-adenylate phosphotransferase (EC 2.7.4.- , Pap, it has been reported in the literature that this enzyme also belongs to the class of Ppk enzymes, but the classification of this enzyme by IUBMB is not very clear)

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