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Method for extracting cordycepin from cordyceps militaris
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An extraction method and technology of cordycepin, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high price, complicated operation, low content, etc., and achieve simple operation, high extraction rate and high purity high effect
Active Publication Date: 2017-01-11
BIOLOGY INST OF HEBEI ACAD OF SCI
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The content of cordycepin in Cordyceps militaris ranges from 1‰-8‰, and the extraction efficiency is not high. Therefore, the production cost of cordycepin is extremely high and the price is expensive. With the in-depth study of the pharmacological effects of cordycepin, the international demand for cordycepin is increasing day by day
[0004] At present, the extraction technology of cordycepin mostly relies on leaching and liquid phase preparation and other technologies. Patent CN102746355A obtains high-purity cordycepin by ultra-low temperature pulverization, ultrasonic water extraction, macroporous resin enrichment, and reverse high-performance liquid chromatography purification. Liquid chromatography has a small amount of sample to be processed each time, and must be processed after purification. Due to the introduction of salt into the mobile phase, the operation method is complicated and the recovery rate is low.
[0006] The content of cordycepin in Cordyceps militaris is low. Selecting a suitable enrichment method is the key to extracting cordycepin. The processing steps should not exceed 5 steps. If it is too cumbersome, the recovery rate will be greatly reduced. Not suitable for mass production
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Embodiment 1
[0041] Weigh 34g of dried artificially cultivated Cordyceps militaris, and the content of cordycepin is determined to be 1.7mg / g. After crushing, add twice the volume of petroleumether, seal and magnetically stir for two days, filter, and air-dry the powder of Cordyceps. Add 600ml of 80% ethanol to extract for 2 hours, filter, repeat twice, combine the filtrate, put it on a rotary evaporator, distill under reduced pressure to a volume of about 100ml, no alcohol smell, add water to 200ml, shake well, and filter. Add water to about 300ml, adjust the pH to 12, add 750ml of ethyl acetate to mix with the crude extract, stir magnetically for 30min, let stand for 80min, take the supernatant, extract 5 times, and distill the supernatant under reduced pressure to recover ethyl acetate , add a small amount of water to dissolve, pass through a 0.45um membrane, the volume of the column used is 30ml, pass through the column in two batches according to the actual situation in the laboratory...
Embodiment 2
[0043] Weigh 150g of dried artificially cultivated Cordyceps militaris, the content of cordycepin is 1.7mg / g, add twice the volume of petroleum ether after crushing, seal with magnetic stirring for two days, filter, and air-dry the powder of Cordyceps. Add 3L of 80% ethanol to extract for 2 hours, filter, repeat twice, combine the filtrate, put it on a rotary evaporator, distill under reduced pressure to a volume of about 400ml, no alcohol smell, add water to 800ml, shake well, and filter. Add water to about 1.5L, adjust the pH to 12 with NaOH, carry out the extraction process in two batches according to the actual situation in the laboratory, mix 2.5 times the volume of ethyl acetate with the crude extract, stir magnetically for 30 minutes, let stand for 80 minutes, and take the supernatant , extracted 5 times, distilled the supernatant under reduced pressure to recover ethyl acetate, added a small amount of water to dissolve, passed through a 0.45um membrane, the column volum...
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Abstract
The invention discloses a method for extracting cordycepin from cordyceps militaris, and the method comprises mainly the following steps: degreasingpetroleumether, extracting high concentrationethanol, extracting ethyl acetate, purifying twice D1400 resin, and then obtaining cordycepin with the purity higher than 95%. The method for extracting cordycepin from cordyceps militaris has the advantages of easy operation, high extraction ration for extracting cordycepin, and high degree of purity, meanwhile, the method for extracting cordycepin from cordyceps militaris is suitable for industrialized production, creating conditions for effective utilization of the cordycepin and laying foundation for further development and effective utilization of the cordycepin.
Description
technical field [0001] The invention belongs to the technical field of extracting medicinal active ingredients, and in particular relates to a method for extracting cordycepin from fruit bodies of Cordyceps militaris. Background technique [0002] The structural formula of cordycepin is 3'-deoxyadenosine. It is the first nucleoside antibiotic isolated from fungi. It is soluble in water, methanol and hot ethanol, but insoluble in benzene, butyl ether and chloroform. The maximum ultraviolet absorption wavelength is 259nm . Cordycepin has pharmacological effects such as anti-tumor, anti-inflammation, anti-virus, and neuroprotection, and it also has adjuvant therapeutic effects on lowering cholesterol, lowering blood sugar, and alcoholic liver poisoning; in 1997, the United States began a phase I clinical trial of cordycepin to treat pre-acute and former T-lymphocytic leukemia patients; cordycepin also exhibited strong antifungal and anti-HIV-I virus activity. [0003] At pres...
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