Lactobacillus zeae LZ3 and application thereof in aquaculture

A technology of Lactobacillus maize and strains is applied in the field of fishery ecological safety, which can solve the problems of water body hypoxia, destroying water body ecological balance, affecting photosynthesis of aquatic plants, etc., so as to improve water quality, prevent harm, and eliminate ammonia nitrogen and nitrite. Effect

Pending Publication Date: 2017-02-22
姜光明 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of antibiotics will cause the breeding objects to develop drug resistance and form drug accumulation in the body, which will affect the quality of aquatic products; the use of disinfectants will

Method used

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  • Lactobacillus zeae LZ3 and application thereof in aquaculture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Aeromonas hydrophila antagonistic bacteria search, screening, identification

[0041] (1) Search and screening of antagonistic bacteria against Aeromonas hydrophila

[0042] A) material

[0043] Aeromonas hydrophila strain: The strain was isolated from the blood of crucian carp suffering from bacterial sepsis, and its pathogenicity was confirmed by the return test of healthy crucian carp; it was identified as Aeromonas hydrophila by the BBL Crystl microbial automatic analysis system bacteria (Aeromonas hydrophila).

[0044] Bottom mud: The bottom mud collected from the Wangwei breeding area of ​​Taihu Lake.

[0045] Medium: trypticase soy agar (TSA) medium, nutrient broth (NB) medium, purchased from reagent companies.

[0046] Preparation of TSA medium: Weigh 42g of TSA, dissolve it in a Erlenmeyer flask of 1000ml distilled water, stir to dissolve, sterilize by high-pressure steam at 121°C for 15min, pour it into a petri dish, and store it at 4°C for later...

Embodiment 2

[0058] Trial production of embodiment 2 antagonistic bacteria preparation (fermentation agent)

[0059] According to the biological characteristics of Lactobacillus zea, a suitable medium was screened out, and appropriate fermentation conditions were adopted to make Lactobacillus zea LZ3 into a fermentation broth, and a certain concentration of Aeromonas hydrophila was prepared by adding an appropriate amount of stabilizer. Bacteria antagonistic antimicrobial starter.

[0060] Concretely adopted fermentation conditions are as follows:

[0061] Fermentation strain: Lactobacillus zea LZ3.

[0062] Fermentation medium formula: peptone 10-80 grams, sucrose 20-100 grams, yeast powder 1-10 grams, purified water 1000 ml.

[0063] Fermentation temperature: 28-30°C.

[0064] Fermentation period: 64-70 hours.

[0065] Rotational speed of fermentation shaker: 150-200r / min.

[0066] Stabilizer: 5-10 grams of Tween-20 and 10-20 grams of glycerin per liter of fermentation broth.

[00...

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Abstract

The invention discloses a Lactobacillus zeae LZ3. The preservation number of the above strain is CCTCC NO:M2015652, and the Lactobacillus zeae LZ3 has Aeromonas hydrophila growth or breeding inhibition ability. The strain can be used as an Aeromonas hydrophila antagonistic microorganism to prepare a bacteriostasis preparation which can be applied to the aquaculture in order to prevent harms of the Aeromonas hydrophila to cultured aquatic products; and the strain can be used to grade ammonia nitrogen, nitrite and other harmful substances in aquaculture water as a probiotic preparation in order to improve the culture water quality.

Description

technical field [0001] The invention relates to a strain of Lactobacillus zea, its preparation and its application in inhibiting Aeromonas hydrophila in aquatic products, belonging to the field of fishery ecological safety, and used for inhibiting Aeromonas hydrophila in aquaculture water and degrading the water body Harmful substances such as ammonia nitrogen and nitrite can be removed to improve water quality. Background technique [0002] Freshwater fish bacterial septicemia caused by Aeromonas hydrophila infection, also known as hemorrhagic disease, bacterial hemorrhagic disease, hemorrhagic ascites disease, ascites disease, fulminant epidemic, etc., has been widely prevalent in my country since 1990. It has the most of four: many types of hazards, including silver carp, silver carp, crucian carp, carp, bream, tilapia, yellow-tailed pomfret, dace, grass carp, perch, mandarin fish and almost all freshwater aquaculture fish , and freshwater aquaculture objects such as frog...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04C12R1/225
CPCA61K2035/115C12N1/205C12R2001/225
Inventor 姜光明钱彩源张元柱
Owner 姜光明
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