Beta-agarase and coding gene and application thereof

A kind of agarase and gene technology, which is applied in application, genetic engineering, plant gene improvement, etc., can solve the problems of difficult large-scale production, instability, low activity, etc., and achieve easy purification, good enzyme activity, and good thermal stability Effect
CN106544333AInactive Publication Date: 2017-03-29XINXIANG MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XINXIANG MEDICAL UNIV
Publication Date
2017-03-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to beta-agarase and a coding gene and application thereof. The amino acid sequence of the beta-agarase is shown in the sequence table SEQ ID No:1. The nucleotide sequence of the coding gene of the beta-agarase is shown in the sequence table SEQ ID No:2. The beta-agarase has the good heat stability and enzymatic activity and can specifically degrade agarose, generate a single end-product of neoagarotetraose and provide a new enzyme source for preparation of agaro-oligosaccharide.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a β-agarase and its coding gene and application. Background technique

[0002] The use of biotechnology to obtain medicines and functional foods from the ocean has attracted increasing attention. Macroalgae polysaccharide——agar (agar) is the main component of the cell wall of red algae. In recent years, with the in-depth study of glycobiology, it has been found that its degradation product agaro-oligosaccharide (AOS) has antibacterial, Anti-virus, value-added intestinal probiotics, anti-tumor, improving body immunity, anti-inflammation, anti-oxidation, scavenging hydroxyl free radicals, moisturizing and whitening and other special biological activities, making it widely used in medicine, food, cosmetics, etc. The industry has high application value.

[0003] Traditional preparation methods of agar oligosaccharides include physical and chemical methods, wherein physical methods main...

Claims

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