Biological surfactant produced by Enterobacter hormaechei and applications thereof

A technology of Enterobacter hallii, biological surface, applied in the application, microorganism, microorganism and other directions, can solve the problems of pollution, environmental pollution, etc., to achieve the effect of promoting absorption, improving absorption efficiency, and preventing retention

Active Publication Date: 2017-04-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have their disadvantages, such as causing environmental pollution or water pollution, and hydrolyzing waste livestock and poultry acid to produce agricultural amino acids, which not only solves the above problems in a harmless manner, but also provides abundant raw materials for the development of new bio-organic fertilizers

Method used

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  • Biological surfactant produced by Enterobacter hormaechei and applications thereof
  • Biological surfactant produced by Enterobacter hormaechei and applications thereof
  • Biological surfactant produced by Enterobacter hormaechei and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Observation of NJAU B4 strain

[0034] The colonies are round, with smooth and raised surfaces. The edge is smooth, the colony is white and slightly transparent, and does not produce pigment; the bacteria have flagella, are short rod-shaped, Gram staining is negative, and do not produce spores.

[0035] 2. Preparation of Seed Solution

[0036]Use a 250mL Erlenmeyer flask to pack 20%-30% (v / v) liquid LB medium, use a flame-sterilized inoculation loop to pick up a small amount of NJAU B4 bacteria preserved in a test tube, shake it in the Erlenmeyer flask Bacteria enter the liquid culture medium. Put the inoculated Erlenmeyer flask into a shaking incubator, the condition is 37°C, the rotation speed is 170rpm, and the cultivation time is about 24h.

[0037] Both the LB medium and the fermentation medium were sterilized at 115°C for 30 minutes in a high-temperature steam sterilizer.

Embodiment 2

[0038] The optimization of the production condition of embodiment 2 tensio-active agent

[0039] 2.1 Days optimization experiment

[0040] In order to determine the optimal number of days for fermentation, various indicators of the fermentation broth are measured. Original fermentation medium formula: NaNO 3 4.0g / L, CaCl 2 ·H 2 O 0.1g / L, NaHPO 4 2H 2 O 3.0g / L, KH 2 PO 4 3.0g / L, MgSO 4 ·7H 2 O0.2g / L, yeast extract 2.0g / L, glycerol 3% (v / v), 2mL trace element solution per liter of fermentation medium; wherein trace element solution formula: NaMoO 4 2H 2 O 0.05g / L, FeCl 3 ·6H 2 O 0.08g / L, ZnSO4 7H 2 O 0.75g / L, CoCl 2 ·6H 2 O 0.08g / L, FeSO 4 ·7H 2 O 0.5g / L, CuSO 4 ·5H 2 O 0.075g / L, MnSO 4 ·H 2 O 0.75g / L, H 3 BO 3 0.15g / L. The number of days of fermentation is optimized with the pH, OD600 of the fermentation broth, the surface tension value of the supernatant after removing the bacteria, and the dry matter weight of the bacteria. The experimental results...

Embodiment 3

[0048] Embodiment 3 Surfactant yield measurement

[0049] 3.1 Determination of direct freeze-dried content

[0050] Measure 1000mL of the supernatant, divide it into 8 tissue culture bottles that have been weighed, pre-freeze in a -80°C refrigerator for 4 to 5 hours, and freeze-dry in a freeze dryer. After lyophilization, its weight was weighed, and it was measured that there were 12.14 g of lyophilized substance in 1 L.

[0051] 3.2 Determination of minimum surface tension value of aqueous solution

[0052] Dissolve 10g of lyophilized product in 10mL of water, measure the surface tension value of the solution with a platinum ring surface tensiometer at room temperature 20°C to 34.14mN / m, then add 1g of lyophilized product, and continue the measurement, the value does not become lower.

[0053] 3.3 Determination of purer surfactant

[0054] (1) Extraction: adjust the pH of the supernatant to 2.0 with 1mol / L HCl solution, then extract with a 2:1 (v / v) mixed solvent of chloro...

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Abstract

The invention discloses a biological surfactant produced by Enterobacter hormaechei and applications thereof. Enterobacter hormaechei is used to produce the biological surfactant. The formula and the production conditions are deeply explored. The addition amounts of carbon source and nitrogen source are improved, the production and fermentation conditions are improved, and thus the yield of surfactant can reach more than 2 grams for each liter. The biological surfactant and waste livestock and poultry amino acid hydrolysate are innovatively compounded, the nutrient loss is avoided, and the purification treatment is not needed.

Description

technical field [0001] The invention belongs to the field of biological reagent production, and relates to a biosurfactant produced by Enterobacter hallii and its application. Background technique [0002] Biosurfactants are a class of surface-active substances with different structures produced by microbial metabolism. Structurally it is an amphiphilic molecule, usually containing a hydrophilic group and a hydrophobic group. The hydrophobic part is usually a saturated or unsaturated carbon chain, and the hydrophilic part is variable, including monosaccharides, low sugars, polysaccharides, short peptides, phosphate groups, etc. Therefore, biosurfactants have different structures and types. According to their biochemical characteristics and molecular weight, etc., they can usually be divided into several categories such as glycolipids, phospholipids and fatty acids, lipopeptides or lipoproteins, polymer surfactants and particle surfactants. Surfactants all have the effect o...

Claims

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Application Information

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IPC IPC(8): C12P1/04C12N1/20C05G3/00C12R1/01
CPCC05C11/00C05G3/00C12P1/04C05G5/20C12N1/205C12R2001/01
Inventor 王权沈其荣刘东阳王佳音朱瀚
Owner NANJING AGRICULTURAL UNIVERSITY
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