Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia
A technology for fermenting feed and shiitake mushroom residues, applied in animal feed, animal feed, bifidobacteria, etc., can solve problems such as waste of agricultural organic resources and environmental pollution, and achieve the effects of reducing fat content, improving taste, and utilizing edible health.
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[0022] (1) Preparation of Bacillus subtilis bacterial liquid:
[0023] Under sterile conditions, Bacillus subtilis CICC 23659 was inserted into the culture medium to carry out liquid fermentation production, wherein the culture medium was calculated as: starch 5%, potassium humate 1%, Soybean cake flour 7.5%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, magnesium sulfate 0.05%, ferric chloride 0.005%, calcium carbonate 0.05%, yeast powder 0.01%, boric acid 0.005%, pH adjusted to 7.2~7.4 , sterilize at about 120°C for 20 min;
[0024] During the fermentation process, the culture temperature range is 28~40℃, and sterile air (dissolved oxygen) is introduced and stirred. The ventilation volume of the sterile air is 0.5~1.0L∕L·min, the stirring speed is 150~200rpm, and the tank pressure is 0.5kg∕cm 2 , oxygen-consuming culture for 48 hours, the number of Bacillus subtilis subsp. stuartii in the obtained bacterial liquid is ≥ 2.0 × 10 9 cfu / mL;
[0025] (2) Prepara...
Embodiment 1
[0035] The preparation method of Lentinus edodes mushroom residue fermented feed, comprises the following steps:
[0036] The first step, in parts by weight, take 55 parts of shiitake mushroom planting slag, pulverize, add 5 times the weight of water, add 3 parts of protease and 2 parts of cellulase, heat for enzymatic hydrolysis, then kill the enzyme, and let it cool to obtain enzymatic solution;
[0037] Step 2: Concentrate the volume of the enzymatic hydrolysis solution to 20%, then add 20 parts of meal (a mixture of soybean meal and cottonseed meal in a weight ratio of 1:1), 15 parts of potato starch, and then add Bacillus subtilis bacterial liquid 1 part, mixed evenly, and then covered with film after stacking, and then fermented, the fermentation temperature is 30°C, the fermentation time is 20 days, and after the fermentation is completed, it is air-dried until the moisture content is 10wt%;
[0038] In the third step, compound vitamins (a mixture of vitamin B and vita...
Embodiment 2
[0040] The preparation method of Lentinus edodes mushroom residue fermented feed, comprises the following steps:
[0041] The first step, in parts by weight, take 65 parts of shiitake mushroom planting residue, pulverize, add 8 times the weight of water, then add 7 parts of protease and 4 parts of cellulase, heat for enzymolysis, then kill the enzyme, and let it cool to obtain enzymatic solution;
[0042] Step 2: Concentrate the volume of the enzymatic hydrolysis solution to 30%, then add 35 parts of meal (a mixture of soybean meal and cottonseed meal in a weight ratio of 1:1), 20 parts of potato starch, and then add Bacillus subtilis bacterial liquid 3 parts, mixed evenly, and then covered with film after stacking, and then fermented, the fermentation temperature was 40°C, and the fermentation time was 30 days. After the fermentation was completed, it was air-dried until the moisture content was 15wt%;
[0043] In the third step, compound vitamins (a mixture of vitamin B and...
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