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Method for acquiring functional components in morinda citrifolia as well as extract and application

A technology of functional ingredients and noni fruit, applied in the field of improving the acquisition of functional ingredients in noni fruit, can solve the problems of waste of research time and cost, difficulty in applying large-scale production, etc., and achieve the effect of increasing the content

Inactive Publication Date: 2017-05-24
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth emphasizing that the research idea of ​​using known factors that can affect the extraction efficiency (such as temperature, extraction reagents, and extraction time) has the following disadvantages: due to the different systems of small-scale, pilot-scale and large-scale production, the level of small-scale (Laboratory level) The combination of parameters with shortcomings is often difficult to apply to large-scale production, and further adjustments are needed, causing a certain waste of research time and costs

Method used

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  • Method for acquiring functional components in morinda citrifolia as well as extract and application
  • Method for acquiring functional components in morinda citrifolia as well as extract and application
  • Method for acquiring functional components in morinda citrifolia as well as extract and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of noni fruit powder: pick different periods (green fruit, ginkgo fruit and mature fruit), dry and crush.

[0033] Weigh 6g of noni fruit powder in a 250mL conical flask, add 60mL of boiling water for extraction for 10 minutes, shake once every 3 minutes, filter under reduced pressure, wash the filter residue with distilled water several times, and dilute the filtrate into a 250mL volumetric flask; accurately draw 1mL of noni Put the Li sample solution in a 25mL colorimetric tube, then add 4mL distilled water and 5mL ferrous tartrate solution, mix well, and finally add pH 7.5 phosphate buffer to the mark. Let it stand for 10-15 minutes, use a 10mm cuvette, and measure the absorbance at a wavelength of 540nm with the reagent blank as a reference.

[0034] The content of total phenols is calculated by the following formula: total phenols (mg / mL) = A×1.957×2

[0035] In the formula: A-the absorbance of the sample

[0036] 1.957——Using a 10mm cuvette, when the abso...

Embodiment 2

[0043] The preparation of noni fruit powder: pick different periods (green fruit, ginkgo fruit and mature fruit), dry and crush.

[0044] Weigh about 2g of noni fruit powder (accurate to 0.0001g) into an Erlenmeyer flask, add 70% ethanol with a material-to-liquid ratio of 1:20, ultrasonicate at 60°C and 100W for 30 minutes, filter, collect the filtrate, and repeat the operation 3 times; Pipette 3mL of the filtrate into a 25mL colorimetric tube, add 70% ethanol to 5mL, add 5% (w / v) NaNO 2 Solution 1mL, shake well, and let stand for 6 min. Plus 10%(w / v)Al(NO 3 ) 3 The solution is 1mL, shake well, and then stand for 6 min, add 10mL of 1.0mol / L NaOH solution, add 70% ethanol solution to 25mL, shake well, stand for 15min, and operate twice in parallel. Measure the absorbance at 510nm wavelength; use the standard curve that has been measured in the laboratory to calculate the total flavonoid content in the sample. The measured linear regression equation is Y=12.921x-0.0005(R 2 =0.9992...

Embodiment 3

[0051] The preparation of noni fruit powder: pick different periods (green fruit, ginkgo fruit and mature fruit), dry and crush.

[0052] Weigh 2.0 g of noni powder, extract with 60 mL of methanol at 70°C and reflux for 1.5 hours each, and then extract the dried product twice with 30 mL of chloroform ultrasonically, with ultrasonic power of 100W, 45°C, 20 minutes each time, and combine the extracts to reduce pressure to After drying, dissolve in a 10 mL measuring flask with methanol, dilute to the mark, filter through a microporous membrane, and take the filtrate for use.

[0053] Chromatographic conditions: C18 column (250mm×4.6mm, 5μm); mobile phase is acetonitrile-1% glacial acetic acid aqueous solution (20:80); flow rate is 1.00mL / min; column temperature is 25℃; detection wavelength is 341nm; Sample volume: 20μL; standard curve: the standard curve is obtained by the determination in 2.10.3, the regression equation is Y=87725X+23464, R 2 = 0.995.

[0054] The experimental data of...

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Abstract

The invention provides a method for acquiring functional components in morinda citrifolia. The method comprises the following steps: firstly, drying and crushing the morinda citrifolia; secondly, extracting by using water or an organic solvent. The method is characterized in that the method comprises the following steps: (A) in the first step, picking up the white morinda citrifolia; (B) in the first step, a drying method adopts infrared drying; the functional components are total phenols, general flavones, total saponins, scopoletin or aucubin. The invention further provides a morinda citrifolia extract prepared according to the method and application of the morinda citrifolia extract in preparation of health-care products and drugs. According to the method provided by the invention, the extraction efficiency of the total phenols, the general flavones, the total saponins, the scopoletin or the aucubin in the morinda citrifolia can be remarkably improved, and the contents of the functional components in the morinda citrifolia extract are remarkably increased.

Description

Technical field [0001] The invention belongs to the field of agricultural product processing, and specifically relates to a method for improving the acquisition of functional components in noni fruit, an extract and an application. Background technique [0002] Noni (Morinda cirtifolia L.) belongs to the genus Morinda in the Rubiaceae family. It is a tropical evergreen perennial small tree or broad-leaved shrub. It is mainly distributed from southwest to southeast China, as well as Taiwan and Hong Kong. [0003] Noni has been proven to have significant effects on many diseases, such as antiviral and anticancer, for the treatment of autoimmune diseases, preventing and reducing the occurrence of acute and chronic pain, and for asthma and other respiratory diseases, diabetes, nephritis, arthritis, hysteria, Allergy, atherosclerosis, blood stasis, digestive system disease, multiple sclerosis, cardiovascular disease (hypertension, myocardial infarction) all have certain effects. [0004]...

Claims

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Application Information

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IPC IPC(8): A61K36/746A23L33/105A23L33/00C07D311/16C07H17/04C07H1/08A61K31/352A61K31/7048A61K131/00
CPCA23V2002/00A61K31/352A61K31/7048A61K36/746A61K2236/15A61K2236/17A61K2236/331A61K2236/333A61K2236/55C07D311/16C07H1/08C07H17/04A23V2200/30A23V2250/21A23V2250/2132A23V2250/2116
Inventor 徐荣杨焱姜士宽徐通曾里郭刚军李海泉龙继明杨朴丽
Owner YUNNAN INST OF TROPICAL CROPS
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