Yarrowia lipolytica having the effect of lowering cholesterol and triglyceride and its application
A technology for lowering cholesterol and yeast strains, which is applied in the fields of application, medical preparations containing active ingredients, fungi, etc., and can solve the problems that yeast has not been reported.
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Embodiment 1
[0021] Screening and identification of Yarrowia lipolytica EAM-ZT003T provided by the present invention with the ability to lower cholesterol and triglyceride.
[0022] 1 Separation and purification of strains
[0023] Weigh 0.5g beer dregs sample, add it into a test tube filled with 4.5ml sterile water, and dilute it to 10 -6 , take 10 -4 、10 -5 、10 -6 0.1ml each of the three tubes of dilution was applied to the McFarland medium for separation by the coating method, and incubated at a constant temperature of 37°C for 48h. Colonies were picked, streaked continuously, separated and purified, and finally a single colony was selected. After microscopic examination confirmed that it was a pure species, it was inoculated into slant medium and cultured for 24 hours, and then stored in a refrigerator at 4°C for later use.
[0024] The formula and conditions of McFarland's medium are: glucose 1.0g, potassium chloride 1.8g, yeast powder 2.5g, anhydrous sodium acetate 8.2g, distille...
Embodiment 2
[0035] The cholesterol-lowering effect of the Yarrowia lipolytica EAM-ZT003T having the ability of lowering cholesterol and triglyceride provided by the present invention.
[0036] 1 Effect of lowering cholesterol bacteria in egg yolk
[0037] Under aseptic conditions, a certain amount of egg yolk is added to the sterilized culture medium as a cholesterol source, and aseptically evenly dispensed into test tubes. With 2% (thalline concentration is 10 8 cfu / ml) was added to the bacterial solution, incubated at 37°C for 24 hours, centrifuged at 8000r / min for 10 minutes, and the supernatant was taken to determine the cholesterol content in the supernatant according to the phosphorus-sulfur-iron colorimetric method. Two repetitions were set for each group of experiments.
[0038] Configuration of ferric sulfur phosphorus color reagent - ferric chloride solution: weigh 5.0g FeCl 3 ·6H 2 O was dissolved in 200ml of concentrated phosphoric acid; phosphorus sulfur iron reagent (P-S...
Embodiment 3
[0054] The triglyceride-lowering effect of the Yarrowia lipolytica EAM-ZT003T with the ability to lower cholesterol and triglyceride provided by the present invention.
[0055] Under aseptic conditions, add a certain amount of standard triglycerides to the sterilized medium, and aseptically dispense it into test tubes. With 2% (thalline concentration is 10 8 cfu / ml) was added to the bacterial solution (the uninoculated test tube was used as a blank control), incubated at a constant temperature of 37°C for 24 hours, and the triglyceride degradation rate was measured at 420nm.
[0056] Triglyceride reduction rate=(C-A) / C×100%
[0057] In the formula, A is the OD value at 420nm of the culture solution after the fermentation of the strain, and C is the OD value at 420nm of the blank control.
[0058] The ability to lower triglycerides of the strain EAM-ZT003T was determined to be 80% through experiments.
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