Microbial preparation for sludge anaerobic fermentation

A technology of microbial preparations and mixed bacterial solutions, applied in the direction of microorganism-based methods, microorganisms, sludge treatment, etc., can solve the problems of reduced methane production efficiency and waste of resources, and achieve reduced treatment costs, improved efficiency, and improved nitrogen removal performance Effect

Inactive Publication Date: 2017-05-31
王筠涵
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Problems solved by technology

[0004] In the anaerobic digestion process of sludge, although it mainly relies on concentration and dehydration, the organic matter is anaerobically decomposed and converted into biogas, which itself is also a digestion process. In the prior art, the process of generating biogas from sludge mainly relies on methane production Bacteria consume formic acid, acetic acid and methanol, and organic acid substances are produced by digestion and decomposition of organic

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  • Microbial preparation for sludge anaerobic fermentation

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Example Embodiment

[0032] Example 1

[0033] A microbial preparation is prepared, which is composed of a mixed bacterial liquid and a carrier, and the mixed bacterial liquid is prepared from Bacillus pumilus, Enterobacter cloacae, denitrifying bacteria, Paracoccus denitrificans, Bacillus amyloliquefaciens, and Lactococcus lactis.

[0034] The Bacillus pumilus (Bacillus pumilus) ATCC27142;

[0035] The Enterobacter cloacae is ( Enterobacter cloacae ) ATCC 700323;

[0036] The denitrifying bacteria is (Paracoccus pantotrophus) ATCC 35512;

[0037] The Paracoccus denitrificans (Paracoccus denitrificans) ATCC13543;

[0038] The Bacillus amyloliquefaciens is ( Bacillus amyloliquefaciens ) ATCC 23843;

[0039] The Lactococcus lactis (Lactococcus lactis) ATCC 11454.

[0040] The carrier is made by mixing kaolin and chitosan in a weight ratio of 1:3;

[0041] The microbial preparation is a mixture of bacterial liquid and a carrier in a weight ratio of 1:2 to prepare a biological preparation;

[0042] The preparation ...

Example Embodiment

[0045] Example 2

[0046] The sludge sample came from a sewage treatment plant and was processed according to the following steps:

[0047] 1) Adjust the total solid content of the sludge to 8wt%;

[0048] 2). Add the sludge with a total solid content of 8% obtained in the first step to the anaerobic digestion tank, the addition amount is 60% (v / v) of the digestion tank volume, and the digestion tank volume is 8% (v / v) ), add water to 90% of the volume of the digestion tank and adjust the pH to 7.5-7.7;

[0049] The methanogenic inoculum is a biogas fermentation broth;

[0050] 3) Inoculate microbial preparations, add 1% (v / v) of the volume of the digestion tank, stir evenly, and cover;

[0051] 4) Keep the reaction temperature at 30℃, stir once every 10h, add rare earth nitrate while stirring, and react for 36h;

[0052] The amount of rare earth nitrate added is 3 μg of rare earth nitrate per gram of volatile solids (VSS) in the sludge, for a total of 3 additions.

[0053] The rare earth...

Example Embodiment

[0058] Example 3 The influence of bacterial composition on methane increase experiment

[0059] First, take 200kg of sludge from a certain sewage treatment plant and divide it into five equal parts, each of which is 40kg.

[0060] Five aliquots of sludge were placed in five identical airtight reaction vessels, and they were marked as experimental group, control group 1, control group 2, control group 3, and blank control group.

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Abstract

The present invention belongs to the field of microbial technology and discloses a microbial preparation for sludge anaerobic fermentation. The microbial preparation comprises a mixed bacteria liquid and a carrier, wherein the mixed bacteria liquid is prepared from Bacillus pumilus, enterobacter cloacae, denitrifying bacteria, paracoccus denitrificans, bacillus amyloliquefaciens, and lactococcus lactis. The microbial preparation can greatly increase the amount of methane generated by sludge, increase the utilization rate of the sludge, and recycle the sludge.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a microbial preparation for anaerobic fermentation of sludge. Background technique [0002] With the acceleration of my country's urbanization process and small town construction and population increase, the public's requirements for environmental quality are increasing day by day. A large number of sewage treatment plants have been built and put into use, and the amount of sludge that follows is also increasing. Sludge is the product of sewage treatment. It is an extremely complex heterogeneous body composed of organic debris, bacterial cells, inorganic particles, colloids, etc. The main characteristics of sludge are high water content, high organic matter content, and easy It is rotten and smelly, and the particles are finer, the specific gravity is smaller, and it is in a colloidal liquid state. It is a thick substance between liquid and solid and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/04C12R1/07C12R1/01
CPCC02F11/04C12N1/20Y02W10/20
Inventor 王筠涵王化国
Owner 王筠涵
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