Biosynthetic gene cluster of noveltymycin and its application

A biosynthetic and noveltymycin technology, applied in the field of microbial genetic resources and genetic engineering, can solve undiscovered problems
CN106754986BActive Publication Date: 2020-05-08SUZHOU RES INST SHANDONG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUZHOU RES INST SHANDONG UNIV
Publication Date
2020-05-08

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Abstract

The invention belongs to a biosynthetic gene cluster of creatmycinum. A nucleotide sequence of the gene cluster is as shown in SEQ ID No. 1 and is named as cxm; the gene cluster comprises 10 genes related to biosynthesis of coding creatmycinum, wherein 7 genes are biosynthesis-related genes; one gene is a controlling gene, one gene is a resistance gene and one gene is a transport gene; the nucleotide sequences of the genes are as shown in SEQ ID No. 2 to SEQ ID No. 11. The gene function is validated through a test of heterogeneous expression combined with same-box gene deletion. The invention further discloses the gene cluster or an application of coded protein expressed by the gene cluster in catalyzed synthesis of the creatmycinum or analogues thereof, and biosynthesis of the creatmycinum is achieved. The gene and the protein thereof can be used for searching and discovering a compound or the gene or the protein capable of being applied to medicines, industry or agriculture.
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Description

technical field

[0001] The invention belongs to the field of microbial genetic resources and genetic engineering, and in particular relates to a noveltymycin biosynthetic gene cluster, a carrier containing it, a host and an application thereof. Background technique

[0002] Chuangxinmycin (chuangxinmycin, CXM) has structural similarity to tryptophan (see formula (I)), and is a highly efficient selective inhibitor of bacterial tryptophanyl tRNA synthetase (TrpRS).

[0003]

[0004] For example, the IC50 of noveltycin on TrpRS of Escherichia coli is 30 nM, but it has no inhibitory effect on sheep TrpRS at a concentration of 30 μM, showing that noveltycin is a specific inhibitor (Brown et al, 2002). Recent studies have shown that tryptophanyl tRNA synthetase inhibitors (indomycin and noveltymycin) can disrupt the biosynthesis of tryptophan-rich proteins associated with resistance to chloramphenicol, erythromycin, and vancomycin , thereby weakening the resistance of bacteria...

Claims

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