Surface plasma resonance and mass spectrum combined device and application method thereof
A surface plasma and combined technology, which is applied in the field of online combined ion resonance and mass spectrometry devices, can solve the problems of low ionization degree, complicated operation process and intolerance, etc., and achieves strong ionization ability and sample signal. Lifting, well-tolerated effect
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[0028] Example 1: Using the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention to perform surface plasmon resonance-mass spectrometry experiments to detect the interaction of human serum albumin-paracetamol and perform mass spectrometry data in real time Collection. Specific steps are as follows.
[0029] (1) Surface plasmon resonance detection chip surface modification. The detection chip used is a transparent glass plate coated with a gold film. After the clean detection chip is installed in the surface plasmon resonance instrument, the following solutions are circulated on the surface of the chip (gold-plated surface) at a flow rate of 0.2mL / min: A, 4mmol / L 3-mercaptopropionic acid aqueous solution, circulate for 60min; B , EDC and NHS mixed aqueous solution, in which EDC (N-(3-(dimethylamino)propyl)-N'-ethylcarbodiimidehydrochloride) is 12.5g / L, NHS (N-hydroxysuccinimide) is 2.5g / L, circulating for 20min; C, human Serum...
Example Embodiment
[0033] Example 2: Using the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention to perform surface plasmon resonance-mass spectrometry experiments to detect the interaction of human serum albumin- hippuric acid and to collect mass spectrometry data in real time . Specific steps are as follows.
[0034] (1) Surface plasmon resonance detection chip surface modification. The detection chip used is a transparent glass plate coated with a gold film. After the clean detection chip is installed in the surface plasmon resonance instrument, the following solutions are circulated on the surface of the chip (gold-plated surface) at a flow rate of 0.2mL / min: A, 4mmol / L 3-mercaptopropionic acid aqueous solution, circulate for 60min; B , EDC and NHS mixed aqueous solution, in which EDC (N-(3-(dimethylamino)propyl)-N'-ethylcarbodiimidehydrochloride) is 12.5g / L, NHS (N-hydroxysuccinimide) is 2.5g / L, circulating for 20min; C, human Serum alb...
Example Embodiment
[0038] Example 3: Comparison of the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention and the document "Interface for Online Coupling of Surface Plasmon Resonance to Direct Analysis in Real Time Mass Spectrometry (Zhang Y., Li X., Nie H ., Yang L., Li Z., Bai Y., Niu L., Song D., Liu H. Analytical Chemistry 2015, 87, 6505-6509)" based on the implementation of a direct analytical ion source (DART source) Surface plasmon resonance-mass spectrometry combined device, in the analysis of acetaminophen and metronidazole two samples of signal intensity results. Specific steps are as follows.
[0039] (1) Device construction and experimental preparation based on DBDI source. The analyte was acetaminophen and metronidazole, respectively dissolved in PBS buffer (phosphate buffered saline, containing 10mmol / L sodium phosphate, 138mmol / L sodium chloride, 2.7mmol / L potassium chloride, pH 7.4 ), the concentration is 20mmol / L. Set the DBD...
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